Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells.

Many therapies using mesenchymal stem cells (MSC) rely on their ability to produce and release paracrine signals with chemotactic and pro-angiogenic activity. These characteristics, however, are mostly studied under standard in vitro culture conditions. In contrast, various novel cell-based therapies imply pre-seeding MSC into bio-artificial scaffolds. Here we describe human bone marrow-derived MSC seeded in Integra matrices, a common type of scaffold for dermal regeneration (SDR). We show and measured the distribution of MSC within the SDR, where cells clearly establish physical interactions with the scaffold, exhibiting constant metabolic activity for at least 15 days. In the SDR, MSC secrete VEGF and SDF-1α and induce transwell migration of CD34(+) hematopoietic/endothelial progenitor cells, which is inhibited in the presence of a CXCR4/SDF-1α antagonist. MSC in SDR respond to hypoxia by altering levels of angiogenic signals such as Angiogenin, Serpin-1, uPA, and IL-8. Finally, we show that MSC-containing SDR that have been pre-incubated in hypoxia show higher infiltration of endothelial cells after implantation into immune deficient mice. Our data show that MSC are fully functional ex vivo when implanted into SDR. In addition, our results strongly support the notion of hypoxic pre-conditioning MSC-containing SDR, in order to promote angiogenesis in the wounds

IR observations of MS 1054-03: Star Formation and its Evolution in Rich Galaxy Clusters

We study the infrared (IR) properties of galaxies in the cluster MS 1054-03 at z=0.83 by combining MIPS 24 micron data with spectra of more than 400 galaxies and a very deep K-band selected catalog. 19 IR cluster members are selected spectroscopically, and an additional 15 are selected by their photometric redshifts. We derive the IR luminosity function of the cluster and find strong evolution compared to the similar-mass Coma cluster. The best fitting Schechter function gives L*_{IR}=11.49 +0.30/-0.29 L_sun with a fixed faint end slope, about one order of magnitude larger than that in Coma. The rate of evolution of the IR luminosity from Coma to MS 1054-03 is consistent with that found in field galaxies, and it suggests that some internal mechanism, e.g., the consumption of the gas fuel, is responsible for the general decline of the cosmic star formation rate (SFR) in different environments. The mass-normalized integrated SFR within 0.5R_200 in MS 1054-03 also shows evolution compared with other rich clusters at lower redshifts, but the trend is less conclusive if the mass selection effect is considered. A nonnegligible fraction (13%) of cluster members, are forming stars actively and the overdensity of IR galaxies is about 20 compared to the field. It is unlikely that clusters only passively accrete star forming galaxies from the surrounding fields and have their star formation quenched quickly afterward; instead, many cluster galaxies still have large amounts of gas, and their star formation may be enhanced by the interaction with the cluster.Comment: 49 pages, 9 figures, accepted by Ap

Neutrino Oscillations and the Supernova 1987A Signal

We study the impact of neutrino oscillations on the interpretation of the supernova (SN) 1987A neutrino signal by means of a maximum-likelihood analysis. We focus on oscillations between $\overline\nu_e$ with $\overline\nu_\mu$ or $\overline\nu_\tau$ with those mixing parameters that would solve the solar neutrino problem. For the small-angle MSW solution ($\Delta m^2\approx10^{-5}\,\rm eV^2$, $\sin^22\Theta_0\approx0.007$), there are no significant oscillation effects on the Kelvin-Helmholtz cooling signal; we confirm previous best-fit values for the neutron-star binding energy and average spectral $\overline\nu_e$ temperature. There is only marginal overlap between the upper end of the 95.4\% CL inferred range of $\langle E_{\overline\nu_e}\rangle$ and the lower end of the range of theoretical predictions. Any admixture of the stiffer $\overline\nu_\mu$ spectrum by oscillations aggravates the conflict between experimentally inferred and theoretically predicted spectral properties. For mixing parameters in the neighborhood of the large-angle MSW solution ($\Delta m^2\approx10^{-5}\,\rm eV^2$, $\sin^22\Theta_0\approx0.7$) the oscillations in the SN are adiabatic, but one needs to include the regeneration effect in the Earth which causes the Kamiokande and IMB detectors to observe different $\overline\nu_e$ spectra. For the solar vacuum solution ($\Delta m^2\approx10^{-10}\,\rm eV^2$, $\sin^22\Theta_0\approx1$) the oscillations in the SN are nonadiabatic; vacuum oscillations take place between the SN and the detector. If either of the large-angle solutions were borne out by the upcoming round of solar neutrino experiments, one would have to conclude that the SN~1987A $\overline\nu_\mu$ and/or $\overline\nu_e$ spectra had been much softer than predicted by currentComment: Final version with very minor wording changes, to be published in Phys. Rev.

Spitzer view on the evolution of star-forming galaxies from z=0 to z~3

We use a 24 micron selected sample containing more than 8,000 sources to study the evolution of star-forming galaxies in the redshift range from z=0 to z~3. We obtain photometric redshifts for most of the sources in our survey using a method based on empirically-built templates spanning from ultraviolet to mid-infrared wavelengths. The accuracy of these redshifts is better than 10% for 80% of the sample. The derived redshift distribution of the sources detected by our survey peaks at around z=0.6-1.0 (the location of the peak being affected by cosmic variance), and decays monotonically from z~1 to z~3. We have fitted infrared luminosity functions in several redshift bins in the range 0<z<~3. Our results constrain the density and/or luminosity evolution of infrared-bright star-forming galaxies. The typical infrared luminosity (L*) decreases by an order of magnitude from z~2 to the present. The cosmic star formation rate (SFR) density goes as (1+z)^{4.0\pm0.2} from z=0 to z=0.8. From z=0.8 to z~1.2, the SFR density continues rising with a smaller slope. At 1.2<z<3, the cosmic SFR density remains roughly constant. The SFR density is dominated at low redshift (z<0.5) by galaxies which are not very luminous in the infrared (L_TIR<1.e11 L_sun, where L_TIR is the total infrared luminosity, integrated from 8 to 1000 micron). The contribution from luminous and ultraluminous infrared galaxies (L_TIR>1.e11 L_sun) to the total SFR density increases steadily from z~0 up to z~2.5, forming at least half of the newly-born stars by z~1.5. Ultraluminous infrared galaxies (L_TIR>1.e12 L_sun) play a rapidly increasing role for z>~1.3.Comment: 28 pages, 17 figures, accepted for publication in Ap

Dairy Ingredients for Chocolate and Confectionery Products.

End of Project ReportHigh free-fat, spray-dried powders were successfully produced at a lower fat content (40% rather than 56%) using ultrafiltration. Chocolates made from these powders had improved flow properties and superior quality. The stability, viscosity and firmness of toffees were improved by optimising the casein, whey protein and lactose levels of skim milk powders used in their manufacture.Department of Agriculture, Food and the Marin

Can a supernova be located by its neutrinos?

A future core-collapse supernova in our Galaxy will be detected by several neutrino detectors around the world. The neutrinos escape from the supernova core over several seconds from the time of collapse, unlike the electromagnetic radiation, emitted from the envelope, which is delayed by a time of order hours. In addition, the electromagnetic radiation can be obscured by dust in the intervening interstellar space. The question therefore arises whether a supernova can be located by its neutrinos alone. The early warning of a supernova and its location might allow greatly improved astronomical observations. The theme of the present work is a careful and realistic assessment of this question, taking into account the statistical significance of the various neutrino signals. Not surprisingly, neutrino-electron forward scattering leads to a good determination of the supernova direction, even in the presence of the large and nearly isotropic background from other reactions. Even with the most pessimistic background assumptions, SuperKamiokande (SK) and the Sudbury Neutrino Observatory (SNO) can restrict the supernova direction to be within circles of radius $5^\circ$ and $20^\circ$, respectively. Other reactions with more events but weaker angular dependence are much less useful for locating the supernova. Finally, there is the oft-discussed possibility of triangulation, i.e., determination of the supernova direction based on an arrival time delay between different detectors. Given the expected statistics we show that, contrary to previous estimates, this technique does not allow a good determination of the supernova direction.Comment: 11 pages including 2 figures. Revised version corrects typos, adds some brief comment

Improving Underrepresented Minority Student Persistence in STEM.

Members of the Joint Working Group on Improving Underrepresented Minorities (URMs) Persistence in Science, Technology, Engineering, and Mathematics (STEM)-convened by the National Institute of General Medical Sciences and the Howard Hughes Medical Institute-review current data and propose deliberation about why the academic "pathways" leak more for URM than white or Asian STEM students. They suggest expanding to include a stronger focus on the institutional barriers that need to be removed and the types of interventions that "lift" students' interests, commitment, and ability to persist in STEM fields. Using Kurt Lewin's planned approach to change, the committee describes five recommendations to increase URM persistence in STEM at the undergraduate level. These recommendations capitalize on known successes, recognize the need for accountability, and are framed to facilitate greater progress in the future. The impact of these recommendations rests upon enacting the first recommendation: to track successes and failures at the institutional level and collect data that help explain the existing trends

Does physical activity counselling enhance the effects of a pedometer-based intervention over the long-term : 12-month findings from the Walking for Wellbeing in the West study

Peer reviewedPublisher PD

Is Large Lepton Mixing Excluded?

The original \bnum -(or $\bar{\nu}_{\tau}$-) energy spectrum from the gravitational collapse of a star has a larger average energy than the spectrum for \bnue since the opacity of \bnue exeeds that of \bnum (or $\nu_{\tau}$). Flavor neutrino conversion, \bnue $\leftrightarrow$ \bnum, induced by lepton mixing results in partial permutation of the original \bnue and \bnum spectra. An upper bound on the permutation factor, $p \leq 0.35$ (99$\%$ CL) is derived using the data from SN1987A and the different models of the neutrino burst. The relation between the permutation factor and the vacuum mixing angle is established, which leads to the upper bound on this angle. The excluded region, $\sin^2 2\theta > 0.7 - 0.9$, covers the regions of large mixing angle solutions of the solar neutrino problem: ``just-so" and, partly, MSW, as well as part of region of $\nu_{e} - \nu_{\mu}$ oscillation space which could be responsible for the atmospheric muon neutrino deficit. These limits are sensitive to the predicted neutrino spectrum and can be strengthened as supernova models improve.Comment: 20 pages, TeX file. For hardcopy with figures contact [email protected]. Institute for Advanced Study number AST 93/1