The use and effectiveness of the eLib subject gateways: a preliminary investigation

Internet subject gateways were set up under the Electronic Libraries Programme (eLib) in order to address some of the problems of searching the Internet which have been identified by information professionals, i.e. locating relevant, good quality information. This preliminary study examines the extent to which academics in two universities use three eLib subject gateways (EEVL, OMNI and SOSIG). The results are generally encouraging for the eLib programme, but it is necessary for the gateways to be more effectively promoted. The study also found that academics do not have the same misgivings about the general search engines as the information professionals and seem to use them more readily than the gateways

Metabolically active cytochrome P450 CYP1B1 in solid tumours: a novel target for chemotherapeutic intervention.

Cytochrome P450 CYP1B1 is a member of a superfamily of haemoproteins that are central to the oxidative metabolism of a wide variety of endogenous and exogenous compounds. Several of these enzymes have an established role in the metabolic bio-transformation of a variety of anti-cancer drugs. In this study, we demonstrated both CYP1B1 and CPR activity in the microsomal fraction of ovarian and kidney tumours

What do untrained conversation partners know about communicating with people with aphasia?

Background This study was undertaken as part of the final year undergraduate degree programme at De Montfort University. In 2006 Turner & Whitworth identified that teaching conversational partners about supportive communication strategies was a key theme of the three main approaches to conversational therapy; the conversational analysis approach, the supported conversation approach and the conversational coaching approach. This research project was designed to investigate whether potential conversation partners who had not received conversational partner training could identify ways to support conversations with people with aphasia. The results of this investigation may help to inform speech and language therapists about what prospective untrained conversational partners may know about supporting communication with people with aphasia. Method 30 people participated in this small scale study. Participants were asked to look at a DVD clip (Kagan, Winckel, & Shumway (1996)) of a doctor interacting unsuccessfully with a person with aphasia. After seeing this unsuccessful interaction the participants were asked to complete two measures designed to assess knowledge of supportive conversational strategies. For the first knowledge measure participants were asked to think of ways to support conversations with people with aphasia and write these onto a blank mind map. For the second knowledge measure participants were asked to complete the Rayner & Marshall (2003) communication strategy identification questionnaire. Results The mind map knowledge measure elicited 17 communication strategies that participants thought might support a conversation with a person with aphasia. Some strategies were thought to be less helpful than others. The responses to the second measure, the Rayner & Marshall (2003) strategic questionnaire, showed that participants were able to differentiate between supportive and unsupportive strategies with 75% accuracy. Discussion The results of this small scale undergraduate study suggest that people who volunteer for conversational partner training may have quite detailed knowledge about how to support conversations with people with a aphasia before undergoing training. The responses elicited in the study suggest that the blank mind map technique and the Rayner & Marshall (2003) strategic questionnaire may be used to gauge the prior knowledge of conversational therapy trainees before conversational therapy begins.. References Kagan, A., Winckel, J. & Shumway, E. (1996). Supported conversation for aphasic adults: Increasing communicative access. DVD. Toronto: Aphasia Centre, North York. Rayner, H. & Marshall, J. (2003) Training volunteers as conversation partners for people with aphasia. International Journal of Language and Communication Disorders, 38, 2, 149-164. Turner, S. & Whitworth, A. (2006) Conversational partner training programmes in aphasia: A review of key themes and participant roles. Aphasiology, 20, 6, 483-510

A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus

Open Access Journal; Published online: 29 August 2017A time-course transcriptome analysis of two cassava varieties that are either resistant or susceptible to cassava brown streak disease (CBSD) was conducted using RNASeq, after graft inoculation with Ugandan cassava brown streak virus (UCBSV). From approximately 1.92 billion short reads, the largest number of differentially expressed genes (DEGs) was obtained in the resistant (Namikonga) variety at 2 days after grafting (dag) (3887 DEGs) and 5 dag (4911 DEGs). At the same time points, several defense response genes (encoding LRR-containing, NBARC-containing, pathogenesis-related, late embryogenesis abundant, selected transcription factors, chaperones, and heat shock proteins) were highly expressed in Namikonga. Also, defense-related GO terms of ‘translational elongation’, ‘translation factor activity’, ‘ribosomal subunit’ and ‘phosphorelay signal transduction’, were overrepresented in Namikonga at these time points. More reads corresponding to UCBSV sequences were recovered from the susceptible variety (Albert) (733 and 1660 read counts per million (cpm)) at 45 dag and 54 dag compared to Namikonga (10 and 117 cpm respectively). These findings suggest that Namikonga’s resistance involves restriction of multiplication of UCBSV within the host. These findings can be used with other sources of evidence to identify candidate genes and biomarkers that would contribute substantially to knowledge-based resistance breeding

Gene-expression profiling of microdissected breast cancer microvasculature identifies distinct tumor vascular subtypes

INTRODUCTION: Angiogenesis represents a potential therapeutic target in breast cancer. However, responses to targeted antiangiogenic therapies have been reported to vary among patients. This suggests that the tumor vasculature may be heterogeneous and that an appropriate choice of treatment would require an understanding of these differences. METHODS: To investigate whether and how the breast tumor vasculature varies between individuals, we isolated tumor-associated and matched normal vasculature from 17 breast carcinomas by laser-capture microdissection, and generated gene-expression profiles. Because microvessel density has previously been associated with disease course, tumors with low (n = 9) or high (n = 8) microvessel density were selected for analysis to maximize heterogeneity for this feature. RESULTS: We identified differences between tumor and normal vasculature, and we describe two subtypes present within tumor vasculature. These subtypes exhibit distinct gene-expression signatures that reflect features including hallmarks of vessel maturity. Potential therapeutic targets (MET, ITGAV, and PDGFRβ) are differentially expressed between subtypes. Taking these subtypes into account has allowed us to derive a vascular signature associated with disease outcome. CONCLUSIONS: Our results further support a role for tumor microvasculature in determining disease progression. Overall, this study provides a deeper molecular understanding of the heterogeneity existing within the breast tumor vasculature and opens new avenues toward the improved design and targeting of antiangiogenic therapies

Characterising current agroecological and regenerative farming research capability and infrastructure, and examining the case for a Living Lab network

Agriculture is a major cause of greenhouse gas (GHG) emissions, biodiversity loss, and pollution. Agroecological and regenerative farming have been advocated as alternative approaches that may have fewer negative (or even net positive) environmental impacts than conventional agriculture at farm- and landscape-scales, leading to considerable interest in these approaches (Newton et al. 2020; Bohan et al. 2022; Prost et al. 2023). This report forms the third part of a Defra-funded project Evaluating the productivity, environmental sustainability and wider impacts of agroecological and regenerative farming systems compared to conventional systems. The first part of this project was a rapid evidence review of agroecological and regenerative farming systems and their impacts (Burgess et al. 2023), and the second reported interview findings to examine farmer and stakeholder perspectives on barriers and enablers in agroecological and regenerative farming (Hurley et al. 2023). This third part of the project characterised the current research capability in agroecology and regenerative farming, and explored the potential role of a new ‘living lab’ trial network

From teaching physics to teaching children : beginning teachers learning from pupils

This paper discusses the development of beginning physics teachers' pedagogical content knowledge (PCK) in the context of teaching basic electricity during a one-year Professional Graduate Diploma in Education course (PGDE) and beyond. This longitudinal study used repeated semi-structured interviews over a period of four-and-a-half years. The interview schedule followed a line of development through the secondary school electrical syllabus in Scotland. Fifteen student teachers were interviewed during the PGDE year. Six of them were followed up at the end of the Induction Year (their first year as a newly qualified teacher), and again two-and-a-half years later. Thematic analysis of the interviews showed that before the beginning teachers had taught any classes, their initial focus was on how to transform their own subject matter knowledge (SMK) about electricity into forms that were accessible to pupils. As the beginning teachers gained experience working with classes, they gave vivid descriptions of interacting with particular pupils when teaching electricity which showed the development of their pedagogical knowledge. This played a significant role in the teachers' change of focus from teaching physics to teaching children as they transformed their SMK into forms that were accessible to pupils and developed their general pedagogical knowledge