904 research outputs found
Lipid exchange promotes fusion of model protocells
Vesicle fusion is an important process underlying cell division, transport,
and membrane trafficking. In phospholipid systems, a range of fusogens
including divalent cations and depletants have been shown to induce adhesion,
hemifusion, and then full content fusion between vesicles. This works shows
that these fusogens do not perform the same function for fatty acid vesicles,
which are used as model protocells (primitive cells). Even when fatty acid
vesicles appear adhered or hemifused to each other, the intervening barriers
between vesicles do not rupture. This difference is likely because fatty acids
have a single aliphatic tail, and are more dynamic than their phospholipid
counterparts. To address this, we postulate that fusion could instead occur
under conditions, such as lipid exchange, that disrupt lipid packing. Using
both experiments and molecular dynamics simulations, we verify that fusion in
fatty acid systems can indeed be induced by lipid exchange. These results begin
to probe how membrane biophysics could constrain the evolutionary dynamics of
protocells.Comment: 15 pages, 7 figure
Dogs as carriers of virulent and resistant genotypes of Clostridioides difficile
While previous research on zoonotic transmission of community-acquired Clostridioides difficile infection (CA-CDI) focused on food-producing animals, the present study aimed to investigate whether dogs are carriers of resistant and/or virulent C. difficile strains. Rectal swabs were collected from 323 dogs and 38 C. difficile isolates (11.8%) were obtained. Isolates were characterized by antimicrobial susceptibility testing, whole-genome sequencing (WGS) and a DNA hybridization assay. Multilocus sequence typing (MLST), core genome MLST (cgMLST) and screening for virulence and antimicrobial resistance genes were performed based on WGS. Minimum inhibitory concentrations for erythromycin, clindamycin, tetracycline, vancomycin and metronidazole were determined by E-test. Out of 38 C. difficile isolates, 28 (73.7%) carried genes for toxins. The majority of isolates belonged to MLST sequence types (STs) of clade I and one to clade V. Several isolates belonged to STs previously associated with human CA-CDI. However, cgMLST showed low genetic relatedness between the isolates of this study and C. difficile strains isolated from humans in Austria for which genome sequences were publicly available. Four isolates (10.5%) displayed resistance to three of the tested antimicrobial agents. Isolates exhibited resistance to erythromycin, clindamycin, tetracycline and metronidazole. These phenotypic resistances were supported by the presence of the resistance genes erm(B), cfr(C) and tet(M). All isolates were susceptible to vancomycin. Our results indicate that dogs may carry virulent and antimicrobial-resistant C. difficile strains
Selection for Replicases in Protocells
PMCID: PMC3649988This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Overturning established chemoselectivities : selective reduction of arenes over malonates and cyanoacetates by photoactivated organic electron donors
The prevalence of metal-based reducing reagents, including metals, metal complexes, and metal salts, has produced an empirical order of reactivity that governs our approach to chemical synthesis. However, this reactivity may be influenced by stabilization of transition states, intermediates, and products through substrate-metal bonding. This article reports that in the absence of such stabilizing interactions, established chemoselectivities can be overthrown. Thus, photoactivation of the recently developed neutral organic superelectron donor 5 selectively reduces alkyl-substituted benzene rings in the presence of activated esters and nitriles, in direct contrast to metal-based reductions, opening a new perspective on reactivity. The altered outcomes arising from the organic electron donors are attributed to selective interactions between the neutral organic donors and the arene rings of the substrates
Toward homochiral protocells in noncatalytic peptide systems
The activation-polymerization-epimerization-depolymerization (APED) model of
Plasson et al. has recently been proposed as a mechanism for the evolution of
homochirality on prebiotic Earth. The dynamics of the APED model in
two-dimensional spatially-extended systems is investigated for various
realistic reaction parameters. It is found that the APED system allows for the
formation of isolated homochiral proto-domains surrounded by a racemate. A
diffusive slowdown of the APED network such as induced through tidal motion or
evaporating pools and lagoons leads to the stabilization of homochiral bounded
structures as expected in the first self-assembled protocells.Comment: 10 pages, 5 figure
System Size, Energy and Centrality Dependence of Pseudorapidity Distributions of Charged Particles in Relativistic Heavy Ion Collisions
We present the first measurements of the pseudorapidity distribution of
primary charged particles in Cu+Cu collisions as a function of collision
centrality and energy, \sqrtsnn = 22.4, 62.4 and 200 GeV, over a wide range of
pseudorapidity, using the PHOBOS detector. Making a global comparison of Cu+Cu
and Au+Au results, we find that the total number of produced charged particles
and the rough shape (height and width) of the pseudorapidity distributions are
determined by the number of nucleon participants. More detailed studies reveal
that a more precise matching of the shape of the Cu+Cu and Au+Au pseudorapidity
distributions over the full range of pseudorapidity occurs for the same
Npart/2A value rather than the same Npart value. In other words, it is the
collision geometry rather than just the number of nucleon participants that
drives the detailed shape of the pseudorapidity distribution and its centrality
dependence at RHIC energies.Comment: Submitted to Physical Review Letter
System size and centrality dependence of charged hadron transverse momentum spectra in Au+Au and Cu+Cu collisions at sqrt(s) = 62.4 and 200 GeV
We present transverse momentum distributions of charged hadrons produced in
Cu+Cu collisions at sqrt(s) = 62.4 and 200 GeV. The spectra are measured for
transverse momenta of 0.25 < p_T < 5.0 GeV/c at sqrt(s) = 62.4 GeV and 0.25 <
p_T < 7.0 GeV/c at sqrt(s) = 200 GeV, in a pseudo-rapidity range of 0.2 < eta <
1.4. The nuclear modification factor R_AA is calculated relative to p+p data at
both collision energies as a function of collision centrality. At a given
collision energy and fractional cross-section, R_AA is observed to be
systematically larger in Cu+Cu collisions compared to Au+Au. However, for the
same number of participating nucleons, R_AA is essentially the same in both
systems over the measured range of p_T, in spite of the significantly different
geometries of the Cu+Cu and Au+Au systems.Comment: 4 pages, 5 figures, submitted to Phys. Rev. Let
System Size, Energy, Pseudorapidity, and Centrality Dependence of Elliptic Flow
This paper presents measurements of the elliptic flow of charged particles as
a function of pseudorapidity and centrality from Cu-Cu collisions at 62.4 and
200 GeV using the PHOBOS detector at the Relativistic Heavy Ion Collider
(RHIC). The elliptic flow in Cu-Cu collisions is found to be significant even
for the most central events. For comparison with the Au-Au results, it is found
that the detailed way in which the collision geometry (eccentricity) is
estimated is of critical importance when scaling out system-size effects. A new
form of eccentricity, called the participant eccentricity, is introduced which
yields a scaled elliptic flow in the Cu-Cu system that has the same relative
magnitude and qualitative features as that in the Au-Au system
Identified charged antiparticle to particle ratios near midrapidity in Cu+Cu collisions at sqrt(s) = 62.4 and 200 GeV
Antiparticle to particle ratios for identified protons, kaons and pions at
sqrt(s) = 62.4 and 200 GeV in Cu+Cu collisions are presented as a function of
centrality for the midrapidity region of 0.2 < eta < 1.4. No strong dependence
on centrality is observed. For the / ratio at ~ 0.51 GeV/c, we
observe an average value of 0.50 +/- 0.003_(stat) +/- 0.04_(syst) and 0.77 +/-
0.008_(stat) +/- 0.05_(syst) for the 10% most central collisions of 62.4 and
200 GeV Cu+Cu, respectively. The values for all three particle species measured
at sqrt(s) = 200 GeV are in agreement within systematic uncertainties with that
seen in both heavier and lighter systems measured at the same RHIC energy. This
indicates that system size does not appear to play a strong role in determining
the midrapidity chemical freeze-out properties affecting the antiparticle to
particle ratios of the three most abundant particle species produced in these
collisions.Comment: 5 Pages, 4 figures Made changes to the figures to include the panel
numbers. Slight changes to the text. Updated data points from other
experiment
Latest Results from PHOBOS
This manuscript contains a summary of the latest physics results from PHOBOS,
as reported at Quark Matter 2006. Highlights include the first measurement from
PHOBOS of dynamical elliptic flow fluctuations as well as an explanation of
their possible origin, two-particle correlations, identified particle ratios,
identified particle spectra and the latest results in global charged particle
production.Comment: 9 pages, 7 figures, PHOBOS plenary proceedings for Quark Matter 200
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