ELISA results using individual <i>T. b. rhodesiense</i> infection and matched control sera.

<p>(A–F) Box plots (generated by Cleveland method) represent the 25^th percentile to the 75^th percentile boundaries in the box with the median line within the box, the whiskers indicate the 10^th and 90^th percentiles. The box plots represent the ELISA signals for each recombinant protein ELISA plate: (A) rISG64-1, (B) rISG64-2, (C) rISG64-3, (D) rISG65-1, (E) rISG65-2 and (F) rISG75) tested against individual sera diluted 1∶1000 from stage 1 <i>T. b. rhodesiense</i> infections (n = 5), stage 2 <i>T. b. rhodesiense</i> infections (n = 20) and matched uninfected controls (n = 20). (G) Heat maps of the same data for the individual sera versus the recombinant protein ELISA plates. (H) Receiver operating characteristics (ROC) plots of the same data. The output statistics for sensitivity and specificity are shown in (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002087#pntd-0002087-t002" target="_blank">Table 2</a>).</p

Sensitivities and specificities of the recombinant ISG ELISAs.

<p>Sensitivities and specificities of the recombinant ISG ELISAs.</p

ELISA results with pooled human sera.

<p>(A) Pooled human sera representing stage 1 <i>T. b. gambiense</i> infections (pool of 10 sera), stage 2 <i>T. b. gambiense</i> infections (pool of 40 sera) and matched uninfected controls (pool of 50 sera) were diluted 1∶1000 and used in triplicate on ELISA plates coated with the rISG75, rISG65-1, rISG65-2, rISG64-1, rISG64-2, rISG64-3 and rGRESAG4a recombinant proteins described in (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002087#pntd-0002087-g002" target="_blank">Figure 2</a>). The mean ELISA signals ± SEM are plotted against the recombinant protein used in the ELISA. (B) As panel A but using pooled human sera representing stage 1 <i>T. b. rhodesiense</i> infections (pool of 5 sera), stage 2 <i>T. b. rhodesiense</i> infections (pool of 20 sera) and matched uninfected controls (pool of 25 sera).</p

Sensitivity and specificity values.

1<p>Taken from <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002087#pntd.0002087-Truc1" target="_blank">[31]</a> for the CAAT test. The values for the rISG65-1 ELISA and lateral flow device using <i>T. b. gambiense</i> infection and control sera were calculated from the data presented in this paper.</p

Performance of the prototype lateral flow device in a blinded study with eighty randomised serum samples.

<p>(A) Visual scores of test line density from rISG65-1 prototype lateral flow devices (scored in increments of 1 from 0 to 5, with very faint test line shadows represented as 0.5) are plotted against the subsequently decoded patient status (stage 1 <i>T. b. gambiense</i> infections (n = 8), stage 2 <i>T. b. gambiense</i> infections (n = 32) and matched uninfected controls (n = 40). (B) The same test strips were removed from the devices and scanned by CAMAG densitometer. The data are plotted directly below the results for the visual scores for the same samples. The R² of a scatter plot was 0.96, showing very good correlation between visual score and CAMAG reading. (C–E) Box plots of the results for the same serum samples analysed by (C) rISG65-1 ELISA, (D) rISG65-1 lateral flow prototype with visual scoring and (E) rISG65-1 lateral flow prototype with CAMAG scanner scoring.</p

ELISA results using individual <i>T. b. gambiense</i> infection and matched control sera.

<p>(A–F) Box plots (generated by Cleveland method) represent the 25^th percentile to the 75^th percentile boundaries in the box with the median line within the box, the whiskers indicate the 10^th and 90^th percentiles. The box plots show ELISA signals for each recombinant protein ELISA plate: (A) rISG64-1, (B) rISG64-2, (C) rISG64-3, (D) rISG65-1, (E) rISG65-2 and (F) rISG75) tested against individual sera diluted 1∶1000 from stage 1 <i>T. b. gambiense</i> infections (n = 10), stage 2 <i>T. b. gambiense</i> infections (n = 40) and matched uninfected controls (n = 50). (G) Heat maps of the same data for the individual sera versus the recombinant protein ELISA plates. (H) Receiver operating characteristics (ROC) plots of the same data. The output statistics for sensitivity and specificity are shown in (<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002087#pntd-0002087-t002" target="_blank">Table 2</a>).</p

Recombinant protein antigens used in this study.

<p>A generic representation of the ISGs is shown at the top and a representation of GRESAG4 is shown at the bottom. All have cleavable N-terminal signal peptides and internal transmembrane domains, typical of type-1 membrane proteins. The constructs prepared and expressed and the soluble proteins successfully purified, are indicated.</p

Trypanosome proteins selectively recognised by <i>T. b. gambiense</i> infection IgG.

<p>Trypanosome proteins selectively recognised by <i>T. b. gambiense</i> infection IgG.</p

ELISA data of recombinant proteins TvY486_0045500 and TvY486_0019690 against pooled <i>T</i>. <i>vivax</i> sera from calves before (day-7) and after infection (day+28).

<p>ELISA data of recombinant proteins TvY486_0045500 and TvY486_0019690 against pooled <i>T</i>. <i>vivax</i> sera from calves before (day-7) and after infection (day+28).</p

Sensitivity and specificity data for <i>T</i>. <i>vivax</i> ISG ELISAs and prototype LFT.

<p>Sensitivity and specificity data for <i>T</i>. <i>vivax</i> ISG ELISAs and prototype LFT.</p