Myxomycetes associated with pipevine, a temperate liana

Coelho IL, Stephenson SL 2012 -Myxomycetes associated with pipevine, a temperate liana. Mycosphere 3(2), 245-249, Doi 10.5943 /mycosphere/3/2/8 Pinevine (Aristolochia macrophylla Lam.), a climbing woody vine native to temperate forests of eastern North America, is morphologically similar to many of the lianas characteristic of moist tropical forests. In August 2010, samples of dead pinevine collected from a study site in the Great Smoky Mountains National Park were used to prepare a series of 50 moist chamber cultures. Thirtyseven of the 50 cultures (74%) yielded evidence (either plasmodia or fruiting bodies) of myxomycetes. Fourteen species representing seven genera were recorded, with members of the Trichiales (41% of all records) and Physarales (49% of all records) the most abundant

Phenology of myxomycetes in Turrialba, Costa Rica

Long-term monitoring and phenological patterns of microbial communities are rare in the scientific literature. Myxomycetes have life cycle characteristics that allow both to be documented. The present study summarizes the integrated floristic and bioclimatic components of a 30-month assessment of myxomycete sporocarps in a premontane tropical forest in Turrialba, Costa Rica. Based on monthly visits and a standard sampling effort of 120 minutes per visit, myxomycetes were recorded on leaves, twigs, and logs on the ground by two to three people in 20-minute periods associated with six different collecting sites within a 34-hectare successional forest patch. Biological data were analyzed using three recorded climatic variables obtained in situ during the complete period of study. Also, the Oceanic Niño Index (ONI), provided by NOAA, an estimate of El Niño-Southern Oscillation (ENSO), was evaluated in the analyses. Overall, 54 species and 2245 records of myxomycetes were recorded, with an average of 14.5 species (range between 6-24) and 78.4 records (range between 20-110) detected each month. In general, neither the number of records nor the number of species were associated with individual climate variables, but multiple regression analyses showed that a combination of the accumulated precipitation of the four days before sampling and the average relative humidity can explain most of the fruiting dynamics (R2 = 0.56). When the ONI index was included in the analyses, the explained variability increased (R2 = 0.64), and when a categorization of months based on the same index was used, analyses showed that both the number of records and species evenness were affected by ENSO. At the species level, Hemitrichia calyculata was the only species observed during every month, closely followed by Arcyria cinerea, A. denudata, and Physarum compressum, recorded on most visits. Sporadic fruiting in some species such as Tubifera microsperma, P. tenerum, P. bogoriense, P. melleum, and Metatrichia vesparia could have been associated with local climate oscillations influenced by ENSO patterns. Phenological patterns were observed at the species level, indicating that in the Neotropics, under favorable conditions, myxomycete sporocarps are practically always present, but species assemblages vary temporally. These variations are primarily driven by local climate, but regional climate dynamics also affect fruiting patterns. Presumably, the remaining ecological effect on fruiting patterns in the Neotropics can be attributed to certain finer factors such as ecosystem structure, substrate quality/ availability, and biotic interactions. As such, phenomena such as climate change can have an important effect on the production of sporocarps by tropical myxomycetes, with subsequent effects of their ecological dynamics.Universidad de Costa Rica/[570-B8-006]/UCR/Costa RicaUCR::Sedes Regionales::Sede del Atlántico::Recinto de Paraíso::Finca Experimental Interdisciplinaria de Modelos Agroecológicos (FEIMA)UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ingeniería::Instituto Investigaciones en Ingeniería (INII

Ecology of Soil Eumycetozoans

Eumycetozoans, commonly referred to as slime moulds, are common to abundant organisms in soils. Three groups of slime moulds (myxogastrids, dictyostelids and protostelids) are recognized, and the first two of these are among the most important bacterivores in the soil microhabitat. The purpose of this paper is first to provide a brief description of all three groups and then to review what is known about their distribution and ecology in soils

Assemblages of myxomycetes associated with three different substrates affected by forest wildfires

Background and aims – In late November and early December of 2016, forest wildfires occurred over portions of the Great Smoky Mountains National Park (USA) and more than 4 000 ha were affected. Previous studies have shown that myxomycete assemblages can be greatly impacted as a result of this type of disturbance; after which, the recovery of the forest determines the availability of substrates for new colonisation. The objective of the project reported herein was to assess the impact of wildfires on the recovery of the assemblages of myxomycetes associated with three different substrates (forest floor leaf litter, the bark of living trees, and woody twigs) in two areas with different fire intensity. Material and methods – Two study areas subjected to different fire intensity were selected and sampled 30 months after the wildfires. Myxomycetes were studied using the moist chamber culture technique as it applies to these organisms. Satellite imagery was used to determine forest recovery and similarity indices were used to compare experimental myxomycete assemblages among study areas and substrates. Historical data were used as a reference to contextualise the results. Key results – A total of 38 species of myxomycetes representing 17 different genera were recorded from the two study areas. Samples from the lower intensity burn area yielded more myxomycetes than samples from the higher intensity burn area, with values of 84% and 59%, respectively. This same pattern was also observed for the number of recorded specimens (133 and 93, respectively). The comparison of experimental assemblages with previous data suggested that ground litter assemblages were still in early stages of recovery, whereas the assemblages associated with bark and twigs had recovered much faster. Conclusion – The relatively higher intensity fire had more of an effect on myxomycetes than the relatively lower intensity fire. Myxomycete assemblages are resilient to wildfires and they recover differentially depending on the substrate they grow on.Slime Mold Project at the University of ArkansasDiscover Life in AmericaUniversidad de Costa Rica /[731-B7-721]/UCR/Costa RicaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ingeniería::Instituto Investigaciones en Ingeniería (INII)UCR::Vicerrectoría de Docencia::Ingeniería::Facultad de Ingeniería::Escuela de Ingeniería de Biosistema

The effect of wildfires on wood-decay fungi in the Great Smoky Mountains National Park

Background: In late November and early December of 2019, wildfires occurred over portions of the Great Smoky Mountains National Park. More than 4,000 ha were affected by the wildfires. The objective of the research reported herein was to access the impact of these wildfires on the assemblages of wood-decay fungi associated with the study areas in the park.Methods: The investigated fungi were confined to two study areas; the first subjected to a relatively high intensity burn and the second subjected to a relatively low intensity burn. In addition to specimens of fungi obtained in the field, small pieces of coarse woody debris were assembled, brought back to the laboratory and placed in plastic chambers for incubation and kept moist. Over the course of two months, fruiting bodies appearing in these incubation chambers were observed and collected. All specimens from both the field and incubation chambers were identified from sequences of the internal transcribed spacer (ITS) region of the ribosomal DNA.Results: A total of 31 different taxa were identified along with nine taxa reported previously as unknown and uncultured. However, almost all of these records were from the study area subjected to the relatively low intensity burn.Conclusion: The relatively high intensity burn site was almost completely devoid of wood-decay fungi.Keywords: Great Smoky Mountains National Park; ITS ribosomal DNA region; Wood-decay fung