Immuno-Electron Microscopical Investigations with a New Tracer: Peroxidase-Labeled Protein A: Application for Detection of Pemphigus and Bullous Pemphigoid Antibodies

Peroxidase-labeled Protein A, a stable immunoenzyme tracer of high reactivity and comparatively low molecular weight, has been applied in immuno-electron microscopy for detection of bound IgG-type pemphigus and bullous pemphigoid antibodies. Comparing Protein A-peroxidase with peroxidase-labeled immunoglobulins, we obtained similar morphological results in corresponding incubation techniques, but lower nonspecific adsorption of Protein A-peroxidase complexes in tissues, The Protein A-peroxidase molecules showed good tissue penetration abilities. Our rapid one-step incubation procedure led to enhanced preservation of tissue fine structures, without the need of prior tissue fixation, It seems that Protein A-peroxidase is able to replace peroxidase-labeled anti-IgG for immuno-electron microscopical purposes

Stability constants of U(VI) and U(IV)-humic acid complexes

Conditional stability constants have been determined for U(IV) and U(VI) Boom Clay humic acid (BCHA) and Aldrich humic acid (AHA) complexes, under anaerobic and carbonate free conditions. The constants are needed for nuclear waste repository performance assessment purposes. The U(IV) constants were obtained by developing an approach based on the solubility product of amorphous U(OH)4. The U(VI) constants were obtained by applying the Schubert ion exchange approach

Bridging the Gaps: Building a University Link Tank

This project proposed a University Link Tank as a mechanism by which to build bridges between employees via a network of small groups. Participation in the Link Tank would be voluntary and open to all VCU and Health System employees. The program would be modeled similarly to the Faculty Learning Community (FLC) program, which is administered by the Center for Teaching Excellence, but it would concentrate on issues that are not specifically related to teaching. Topics will be proposed annually. The program would also concentrate on effectively utilizing existing VCU resources to resolve issues