Management and particular ultrasonographic finding in childhood acute scrotal swelling and pain associated with Henoch-Schönlein purpura: A case report and review of the literature

Henoch–Schönlein purpura (HSP), also known as immunoglobulin A (IgA) vasculitis, is a systemic vasculitis of unknown cause, mostly affecting children, in which complexes of IgA and components of complement are deposited on arterioles, capillaries, and venules. It is characterized by nonthrombocytopenic palpable purpura, abdominal pain, and arthritis. While renal involvement is well-known and frequent, scrotal involvement is uncommon and, therefore, not easy to be interpreted. Here, we report a case of a 3-year-old boy hospitalized for HSP, presenting with bilateral acute scrotum, and discuss diagnosis and treatment

Management and particular ultrasonographic finding in childhood acute scrotal swelling and pain associated with Henoch-Schönlein purpura: A case report and review of the literature

Henoch–Schönlein purpura (HSP), also known as immunoglobulin A (IgA) vasculitis, is a systemic vasculitis of unknown cause, mostly affecting children, in which complexes of IgA and components of complement are deposited on arterioles, capillaries, and venules. It is characterized by nonthrombocytopenic palpable purpura, abdominal pain, and arthritis. While renal involvement is well-known and frequent, scrotal involvement is uncommon and, therefore, not easy to be interpreted. Here, we report a case of a 3-year-old boy hospitalized for HSP, presenting with bilateral acute scrotum, and discuss diagnosis and treatment

The Role of Glutamine Synthetase (GS) and Glutamate Synthase (GOGAT) in the Improvement of Nitrogen Use Efficiency in Cereals

Cereals are the most broadly produced crops and represent the primary source of food worldwide. Nitrogen (N) is a critical mineral nutrient for plant growth and high yield, and the quality of cereal crops greatly depends on a suitable N supply. In the last decades, a massive use of N fertilizers has been achieved in the desire to have high yields of cereal crops, leading to damaging effects for the environment, ecosystems, and human health. To ensure agricultural sustainability and the required food source, many attempts have been made towards developing cereal crops with a more effective nitrogen use efficiency (NUE). NUE depends on N uptake, utilization, and lastly, combining the capability to assimilate N into carbon skeletons and remobilize the N assimilated. The glutamine synthetase (GS)/glutamate synthase (GOGAT) cycle represents a crucial metabolic step of N assimilation, regulating crop yield. In this review, the physiological and genetic studies on GS and GOGAT of the main cereal crops will be examined, giving emphasis on their implications in NUE

GUN1 involvement in the redox changes occurring during biogenic retrograde signaling

Chloroplast biogenesis requires a tight communication between nucleus and plastids. By retrograde signals, plastids transmit information about their functional and developmental state to adjust nuclear gene expression, accordingly. GENOMES UNCOUPLED 1 (GUN1), a chloroplast-localized protein integrating several developmental and stress-related signals, is one of the main players of retrograde signaling. Here, we focused on the interplay between GUN1 and redox regulation during biogenic retrograde signaling, by investigating redox parameters in Arabidopsis wild type and gun1 seedlings. Our data highlight that during biogenic retrograde signaling superoxide anion (O2-) and hydrogen peroxide (H2O2) play a different role in response to GUN1. Under physiological conditions, even in the absence of a visible phenotype, gun1 mutants show low activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX), with an increase in O2- accumulation and lipid peroxidation, suggesting that GUN1 indirectly protects chloroplasts from oxidative damage. In wild type seedlings, perturbation of chloroplast development with lincomycin causes H2O2 accumulation, in parallel with the decrease of ROS-removal metabolites and enzymes. These redox changes do not take place in gun1 mutants which, in contrast, enhance SOD, APX and catalase activities. Our results indicate that in response to lincomycin, GUN1 is necessary for the H2O2-dependent oxidation of cellular environment, which might contribute to the redox-dependent plastid-to nucleus communication

Chloroplast-localized {GUN}1 contributes to the acquisition of basal thermotolerance in Arabidopsis thaliana

Heat stress (HS) severely affects different cellular compartments operating in metabolic processes and represents a critical threat to plant growth and yield. Chloroplasts are crucial for heat stress response (HSR), signaling to the nucleus the environmental challenge and adjusting metabolic and biosynthetic functions accordingly. GENOMES UNCOUPLED 1 (GUN1), a chloroplast-localized protein, has been recognized as one of the main players of chloroplast retrograde signaling. Here, we investigate HSR in Arabidopsis wild-type and gun1 plantlets subjected to 2 hours of HS at 45 degrees C. In wild-type plants, Reactive Oxygen Species (ROS) accumulate promptly after HS, contributing to transiently oxidize the cellular environment and acting as signaling molecules. After 3 hours of physiological recovery at growth temperature (22 degrees C), the induction of enzymatic and non-enzymatic antioxidants prevents oxidative damage. On the other hand, gun1 mutants fail to induce the oxidative burst immediately after HS and accumulate ROS and oxidative damage after 3 hours of recovery at 22 degrees C, thus resulting in enhanced sensitivity to HS. These data suggest that GUN1 is required to oxidize the cellular environment, participating in the acquisition of basal thermotolerance through the redox-dependent plastid-to-nucleus communication