THE FERMENT SYSTEMS OF THE PROSTANOIDS SYNTHESIS. THE PREPARATIVE RECEPTION OF THE NATURAL COMPOUNDS AND THOSE MODIFIED BY TRITIUM

The new method of receiving of the prostaglandine syntase has been developed, the kinetics of the biferment reactions of synthesis of prostaglandines and thromboxan has been developed, the mathematical model of the ferment systems of the prostanoids synthesis has been built and analyzed. The optimum conditions of synthesis of prostaglandines and thromboxan have been developed, 27 natural compounds and those modified by tritium have been obtained. The results of the work can be used in the investigations of the Higher SchoolsAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose

Poly-(ADP-ribosyl)-ation (PARylation) is a reversible post-translational modification of proteins and DNA that plays an important role in various cellular processes such as DNA damage response, replication, transcription, and cell death. Here we designed a fully genetically encoded fluorescent sensor for poly-(ADP-ribose) (PAR) based on Förster resonance energy transfer (FRET). The WWE domain, which recognizes iso-ADP-ribose internal PAR-specific structural unit, was used as a PAR-targeting module. The sensor consisted of cyan Turquoise2 and yellow Venus fluorescent proteins, each in fusion with the WWE domain of RNF146 E3 ubiquitin ligase protein. This bipartite sensor named sPARroW (sensor for PAR relying on WWE) enabled monitoring of PAR accumulation and depletion in live mammalian cells in response to different stimuli, namely hydrogen peroxide treatment, UV irradiation and hyperthermia