53 research outputs found
Estrus Induction in Sexually Mature Gilts with Different Hormonal Treatments
The aim of this study was to determine the effectiveness of the synchronized estrus induction in sexually mature gilts, after treatment with different hormonal treatments, given in the unknown phase of a spontaneous estrus cycle. Sexually mature gilts were used, with at least one spontaneous estrous cycle. A total of 90 gilts were treated with single i/m injection of 1,000 IU eCG (Folligon®), 40 gilts were treated with two separate i/m injection of 1 ml PGF2α (Dinolytic®), at an interval of 11 days, and 40 gilts were treated orally (within diet) with 20 mg of synthetic progestagen preparation Altrenogest (Regumate®), during 18 days. Estrus was detected twice daily, in the 10h to 12h intervals, by direct contact with the teaser boar. The best induction of synchronized estrus (in 90% of gilts), which appeared within the first 7 days (mean 5.3 days) after treatment, was found in gilts treated with progestagen preparations. In the three repetitions of treatment with eCG, within the first 7 days (mean 4.2 days) after the treatment, estrus was detected in 65%, 40% or 33% of the treated gilts. After treatment with luteolytic preparation PGF2α (Dynolitic®), estrus was detected in 40% of gilts, on average 5.5 days after treatment. The obtained results clearly show that the successful induction of synchronized estrus in sexually mature gilts can be done only by the method for prolongation of the luteal phase of the spontaneous estrous cycles, using progestagen preparations
Phenotypic Relation Growth Rate in Performance Test and Litter Size of Sows
In this study we investigated influence of weight gain level of gilts reached during performance test on the expression of important reproductive traits. For the purpose of research, data on the litter size from 2nd to 9th parity of 700 tested gilts were monitored. Characteristics that were examined are: growth at the end of the test, the number of piglets born alive, stillborn and weaned piglets per parity and a total from 2nd to 9th parity, and the percentage of sows entering the next farrowing. Results obtained in this study indicate that gilts with a lower weight gain in performance test have smaller number of live born (8.8:9.23 p<0.01) and reared piglets (8.46:8.55, p<0.01) in regard to the higher weight gain gilts, but a larger number of stillborn piglets (0.35:0.29). Also, gilts with the lowest weight gain during the performance test had smaller average litter size from 2nd to 9th parity. Even greater difference is determined if the average litter size from 3rd to 7th parity (9.8:10.0 and 10.4) is observed. The largest percentage of sows farrowing for the second time was in gilts with the highest weight gain during the performance test (39.5%: 14.8% and 15.5%) which expressed statistically significant difference (p<0.01). Gilts with a lower gain weight in performance test had a higher percentage of sows included in the next farrowing. Data showed a large drop in the number of sows with the second and third parity in the total sample, where the percentages of earned second and third litters were 16.5 and 45.3%, while the percentages of actual parity from the 4th to 7th parity were: 79.2; 94.7; 83.3; 96.7; 72.4 and 90.5%
Litters Health Status and Growth Parameters in the Sows Feeding Diets Supplemented with Probiotic Actisaf Sc 47® within Pregnancy Or Lactation
The aim of this study was to investigate the effect of supplementing standard diets for pregnant and lactating sows with live yeast culture (Saccharomyces cerevisiae) on their health status, as well as the health status and growth parameters of their litters during lactation. A total of 120 sows were divided into three groups: the first group was fed diets supplemented with probiotics during pregnancy (G+P, n=40), the second group was fed these diets during lactation (L+P, n=40), and the third group was the control group which was not fed diets supplemented with probiotics (C, n = 40). During the lactation period, a significantly (p<0.01) smaller proportion of probiotic treated sows (G+P=7.5%, L+P=12.5%) manifested clinical signs of the uterus and/or the udder disease in comparison with the control sows (22.5%). The incidence of infectious diarrhea in the nursing piglets was significantly (p<0.05) lower in the treated sows (12.5%) compared to the control sows (27.5 %). The average number of weaned piglets per litter (p/l) and average litter weight at weaning (lw) (G+P=11.6 p/l and 103.6 kg lw, L+P=11.1 p/l and 102.8 kg lw, C=10 p/l and 79 kg lw) were significantly higher (p<0.01 or p<0.05) in sows treated with probiotic compared to the control sows. These results clearly show that the use of probiotic significantly improves the health status of sows and nursing piglets, as well as the piglets growth parameters
Fractal and stereological analyses of insulin-induced rat exocrine pancreas remodelling
Background: The effect of insulin on the endocrine pancreas has been the subject of extensive study, but quantitative morphometric investigations of the exocrine pancreas are scarce. This study was therefore undertaken to investigate the effect of acute and chronic insulin administration (two doses, 0.4 IU and 4 IU) on the morphology of rat pancreas acini. Materials and methods: Semi-fine sections stained with methylene blue and basic fuchsine or haematoxylin and eosin-stained 5-micrometer thick paraffin sections were used for fractal and stereological analysis of exocrine acini. Acute insulin treatment, independent of applied doses increased fractal dimension in line with decreased lacunarity of pancreas acini. Chronic low dose insulin decreased fractal dimension and increased lacunarity of pancreas acini, but a high dose had the opposite effect. The volume densities (Vv) of cytoplasm, granules and nucleus are affected differently: acute low dose and high chronic dose significantly decreased granules Vv, and in line increased cytoplasmic Vv, whereas other examined structures showed slight changes without statistical significance. Results: The results obtained from this investigation indicate that insulin treatment induced structural remodelling of the exocrine pancreas suggesting a substantial role of insulin in its functioning. Conclusions: Additionally, we showed that fine architectural changes in acini could be detected by fractal analysis, suggesting this method as an alternative or addition to routine stereology
Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-α
The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsuli-naemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-α) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone
Ki67 index is an independent prognostic factor in epithelioid but not in non-epithelioid malignant pleural mesothelioma: a multicenter study
BACKGROUND: Estimating the prognosis in malignant pleural mesothelioma (MPM) remains challenging. Thus, the prognostic relevance of Ki67 was studied in MPM. METHODS: Ki67 index was determined in a test cohort of 187 cases from three centres. The percentage of Ki67-positive tumour cells was correlated with clinical variables and overall survival (OS). The prognostic power of Ki67 index was compared with other prognostic factors and re-evaluated in an independent cohort (n=98). RESULTS: Patients with Ki67 higher than median (>15%) had significantly (P<0.001) shorter median OS (7.5 months) than those with low Ki67 (19.1 months). After multivariate survival analyses, Ki67 proved to be-beside histology and treatment-an independent prognostic marker in MPM (hazard ratio (HR): 2.1, P<0.001). Interestingly, Ki67 was prognostic exclusively in epithelioid (P<0.001) but not in non-epithelioid subtype. Furthermore, Ki67 index was significantly lower in post-chemotherapy samples when compared with chemo-naive cases. The prognostic power was comparable to other recently published prognostic factors (CRP, fibrinogen, neutrophil-to-leukocyte ratio (NLR) and nuclear grading score) and was recapitulated in the validation cohort (P=0.048). CONCLUSION: This multicentre study demonstrates that Ki67 is an independent and reproducible prognostic factor in epithelioid but not in non-epithelioid MPM and suggests that induction chemotherapy decreases the proliferative capacity of MPM
Disturbance indicator values for European plants
Motivation Indicator values are numerical values used to characterize the ecological niches of species and to estimate their occurrence along gradients. Indicator values on climatic and edaphic niches of plant species have received considerable attention in ecological research, whereas data on the optimal positioning of species along disturbance gradients are less developed. Here, we present a new data set of disturbance indicator values identifying optima along gradients of natural and anthropogenic disturbance for 6382 vascular plant species based on the analysis of 736,366 European vegetation plots and using expert-based characterization of disturbance regimes in 236 habitat types. The indicator values presented here are crucial for integrating disturbance niche optima into large-scale vegetation analyses and macroecological studies. Main types of variables contained We set up five main continuous indicator values for European vascular plants: disturbance severity, disturbance frequency, mowing frequency, grazing pressure and soil disturbance. The first two indicators are provided separately for the whole community and for the herb layer. We calculated the values as the average of expert-based estimates of disturbance values in all habitat types where a species occurs, weighted by the number of plots in which the species occurs within a given habitat type. Spatial location and grain Europe. Vegetation plots ranging in size from 1 to 1000 m(2). Time period and grain Vegetation plots mostly sampled between 1956 and 2013 (= 5th and 95th quantiles of the sampling year, respectively). Major taxa and level of measurement Species-level indicator values for vascular plants. Software format csv file
Corrigendum to "European contribution to the study of ROS:A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)" [Redox Biol. 13 (2017) 94-162]
The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the topics briefly described below. The formation of reactive oxygen and nitrogen species (RONS) is an established hallmark of our aerobic environment and metabolism but RONS also act as messengers via redox regulation of essential cellular processes. The fact that many diseases have been found to be associated with oxidative stress established the theory of oxidative stress as a trigger of diseases that can be corrected by antioxidant therapy. However, while experimental studies support this thesis, clinical studies still generate controversial results, due to complex pathophysiology of oxidative stress in humans. For future improvement of antioxidant therapy and better understanding of redox-associated disease progression detailed knowledge on the sources and targets of RONS formation and discrimination of their detrimental or beneficial roles is required. In order to advance this important area of biology and medicine, highly synergistic approaches combining a variety of diverse and contrasting disciplines are needed
Inclusão escolar do portador de paralisia cerebral: atitudes de professores do ensino fundamental
Wake-on-a-Schedule: Energy-aware Communication in Wi-Fi Networks
Excessive energy consumption of mobile device Wi-Fi (IEEE 802.11x) interface is limiting its operational time
on batteries, and impacts total energy consumption of electronic devices. In recent years research community
has invested great effort in better efficiency of energy consumption. However, there is still a space for improvement.
Wi-Fi devices connected to the single AP (Access Point) compete for the medium during data exchange. However, due
to the performance anomaly in 802.11 networks, a low data rate device will force all other devices connected to the
AP to communicate at low rate, which will increase the total energy consumption of these devices. Wake-on-a-Schedule
algorithm is proposed reducing the energy consumption of devices placed in the area with the weaker signal by
scheduling the data packets for each client on the server side which will not allow clients to compete for
the Wi-Fi medium. Through extensive measurements we show that our algorithm can save up to 60% of energy
consumption on the client side
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