Are plants with anti-cancer activity resistant to crown gall? : A test of hypothesis

The Crown gall tumour assay (CGTA) is one of several bench top bioassays recommended for the rapid screening of plants with anti-cancer activity. The rationale for the use of the bioassay is that the tumorogenic mechanism initiated in plant tissues by _Agrobacterium tumefaciens_ is in many ways similar to that of animals. Several plant species with anti-cancer activity have already been discovered using this bioassay. However till date no explicit test of an association between anti-cancer activity of plants and their resistance to crown gall formation has been demonstrated. Demonstration of an association could have exploratory potential when searching for plants with anti-cancer activity. In this paper, we determined whether or not a statistically significant association between crown gall resistance and anti-cancer activity exists in plants found in existing published data sets. Our results indicate that plants with anti-cancer activity have a higher proportion of their species resistant to crown gall formation compared to a random selection of plants. We discuss the implications of our results especially when prospecting for newer sources of anti-cancer activity in plants

In pursuit of a universal barcode of plants: peril of followers?

In May 2007, during the early days of the DNA bar coding project in India, we published an article, ‘DNA barcoding: an exercise in futility or utility’1. As the title reflects, we were literally at crossroads, caught between the cross-fire of traditional taxonomists (we think it is disrespectful to call them traditional; they are as much modern as are archaeologists and molecular biologists) and molecular systematists and not knowing which way to go forward. After a reasonable amount of brain-storming that took us through well-trodden criticisms of the DNA barcoding initiative, we concluded that while debates can go on, the tool itself can be effectively used in complementing conventional taxonomic studies and in securing Intellectual Property Rights (IPRs) for important taxa. We also felt at that time that it would be important for the country to develop skills and infrastructure to undertake barcoding of at least some of the important taxa, both for conservation and commerce

Малая автоматизация на ЦВМ "Урал-11Б"

Wireless communication technologies like GPRS, UMTS and WLAN, combined with the availability of high-end, affordable mobile devices enable the development of the advanced and innovative mobile services. Devices such as mobile phones and Personal Digital Assistants let the users access a wide range of new offerings whenever and wherever they happen to be. A strategic approach for the quality assurance of these mobile data services should take into account a number of characteristics unique to the mobile paradigm, the increased complexity of emerging handheld devices, the greater sensitivity to security and load related problems in wireless infrastructure and increased complexities of scale. This paper identifies the major factors influencing the development and testing strategies for these applications and accordingly elaborates effective quality assurance principles to ensure productive and scalable mobile data services

Regulation of Branching by Phytochrome and Phytohormones

Light is the fundamental source of energy and information throughout the plant life cycle. Light signals regulate plant architecture and branching, key processes that determine biomass production and grain yield. Low red (R) to far-red (FR) light ratios (R:FR) perceived by phytochromes serve as a warning signal about impending competition for light resources and lead to shade avoidance responses (SARs), including reduced branching. The R:FR regulates branching in both a bud autonomous and non-bud autonomous manner, however a detailed mechanistic understanding of the process remains unclear. We hypothesized that high R:FR promotes bud outgrowth by differentially regulating branching-related genes (transcriptome) within the axillary bud and that increased apical dominance under low R:FR or with phyB deficiency is mediated by auxin or other novel signal/s. We analyzed the branching phenotype of Arabidopsis Columbia-60000 ecotype in response to different R:FR treatments and conducted a microarray study to identify early (within 3 hours) changes in the transcriptome of buds from different rosette positions in response to altered R:FR. Physiological experiments were also conducted to determine if auxin concentration, transport rate, sensitivity, and establishment of an auxin transport stream were important in determining the branching phenotype of shade avoiding plants. The results revealed that the duration of low R:FR determines plant architecture and the branching phenotype and that bud outgrowth is regulated by the R:FR in a spatial and temporal manner. Low R:FR promoted the elongation of branches at top rosette nodes while it suppressed the outgrowth of axillary buds at lower nodes. High R:FR could reverse the effects of previous low R:FR by promoting the outgrowth of buds from lower axils within 24 hours of treatment. Transcriptomic analysis revealed that the R:FR differentially regulated the expression of genes related to hormone biosynthesis/transport/signaling, cell-cycle regulation and cell wall modification. Cis-elements responsive to light and hormone signaling pathways were overrepresented in several gene clusters. Apical dominance related studies discovered that loss of phyB function results in a slower auxin transport rate, fewer xylem parenchyma cells, and reduced sensitivity to auxin. These results, in addition to estimates of correlative inhibition, suggested that auxin is at least partially responsible for increased apical dominance under low R:FR or with phyB deficiency, but may be acting in conjunction with other undefined regulators

О новом роде верхнепалеозойских растений Brovuchkia Parfenova gen. nov. из Кемеровского района Кузбасса

Multimedia information systems have been developed into service-ware. With the paradigms of web services, service oriented architectures (SOA), and Web 2.0 widgets, multimedia has become truly ubiquitous. However, interoperability, scalability, reliability and security are arising challenges at mobile multimedia service development. This paper focuses on the analysis, design, development and evaluation of a middleware that allows access from mobile devices to a bundle of multimedia services. The services are based on the international multimedia metadata description standard MPEG-7. The implementation is based on new generation of service-oriented application servers called Lightweight Application Server (LAS). Mobile web services refer to the fact that mobile servers host web services. A prototype was developed as a proof of concept, showing how to access MPEG-7 based multimedia services from a Mobile Host and the analysis results of providing MPEG-7 based multimedia services in the form of web services from the Mobile Host to other mobile devices. An alternative solution is to apply enterprise service bus technology as the middleware. The performance evaluation results of both approaches show the reliable accessibility of MPEG-7 based multimedia services via the enterprise service bus solution

Deep Reinforcement Learning (DRL)-based Methods for Serverless Stream Processing Engines: A Vision, Architectural Elements, and Future Directions

Streaming applications are becoming widespread across an extensive range of business domains as an increasing number of sources continuously produce data that need to be processed and analysed in real time. Modern businesses are aggressively using streaming data to generate valuable knowledge that can be used to automate processes, help decision-making, optimize resource usage, and ultimately generate revenue for the organization. Despite their increased adoption and tangible benefits, support for the automated deployment and management of streaming applications is yet to emerge. Although a plethora of stream management systems have flooded the open source community in recent years, all of the existing frameworks demand a considerably challenging and lengthy effort from human operators to manually and continuously tune their configuration and deployment environment in order to reach and maintain the desired performance goals. To address these challenges, this article proposes a vision for creating Deep Reinforcement Learning (DRL)-based methods for transforming stream processing engines into self-managed serverless solutions. This will lead to an increase in productivity as engineers can focus on the actual development process, an increase in application performance potentially leading to reduced response times and more accurate and meaningful results, and a considerable decrease in operational costs for organizations.Comment: 21 pages, 10 figure

Development and characterization of microsatellite markers for Phyllanthus emblica Linn., important nontimber forest product species

Phyllanthus emblica and P. indofischeri, commonly known as the Indian gooseberry, are important nontimber forest product (NTFP) species widely distributed across the Indian subcontinent. The fruits of these species are rich in vitamin C and are used in the preparation of a number of herbal medicines for treating a wide range of disorders. Due to the increased demand, they have been harvested extensively and form a major source of income for the forest-dwelling communities living in southern India. There are limited studies to understand the impact of harvesting on the genetic structure of these species. In this study, 15 polymorphic microsatellite markers have been developed for P. emblica and were characterized by screening 20 individuals each of P.emblica and P. indofischeri. The number of alleles per locus ranged 2–9 for P. emblica and 2–11 for P. indofischeri. The observed and expected heterozygosity of P. emblica ranged 0–1 and 0.401–0.825, respectively. Similarly, the observed and expected heterozygosity of P. indofischeri ranged 0.5–1 and 0.366–0.842, respectively. Cross-amplification of the designed primers was assessed with seven related Phyllanthus species. The microsatellite markers developed can be used for studying the population genetic structure, gene flow and genetic diversity of P. emblica and P. indofischeri

Are mini DNA-barcodes sufficiently informative to resolve species identities? An in silico analysis using Phyllanthus

DNA barcodes using certain regions of the genomic DNA have become a popular diagnostic tool to assign species specific signatures. In plants, a number of chloroplast DNA regions such as psbA-trnH of size 400 bp have been shown to successfully discriminate members of various taxa. However, the technique is not always successful, as in the case of museum specimens or samples used in raw drug trade, where the DNA is often degraded. In this context, recent studies have suggested the use of shorter stretches of the region, called mini-barcodes, to resolve species identity. The minibarcodes are relativelymore stable and easily recovered from the degraded DNA. In this study, an attempt has been made to compare the effectiveness of mini-barcodes over full-length DNA barcodes in differentiating 16 species of Phyllanthus (Phyllanthaceae) used in the raw drug trade. Using an in silico approach, mini-barcodes of varying lengths (50–200 bp) of the region psbA-trnH were generated and evaluated for their ability to resolve the 16 Phyllanthus species in comparison to the full-length DNA barcode of size 398 bp. Results have been discussed in the light of the overall utility of mini-barcodes in resolving the species identities

Twenty-First Century Research Needs in Electrostatic Processes Applied to Industry and Medicine

From the early century Nobel Prize winning (1923) experiments with charged oil droplets, resulting in the discovery of the elementary electronic charge by Robert Millikan, to the early 21st century Nobel Prize (2002) awarded to John Fenn for his invention of electrospray ionization mass spectroscopy and its applications to proteomics, electrostatic processes have been successfully applied to many areas of industry and medicine. Generation, transport, deposition, separation, analysis, and control of charged particles involved in the four states of matter: solid, liquid, gas, and plasma are of interest in many industrial and biomedical processes. In this paper, we briefly discuss some of the applications and research needs involving charged particles in industrial and medical applications including: (1) Generation and deposition of unipolarly charged dry powder without the presence of ions or excessive ozone, (2) Control of tribocharging process for consistent and reliable charging, (3) Thin film (less than 25 micrometers) powder coating and Powder coating on insulative surfaces, (4) Fluidization and dispersion of fine powders, (5) Mitigation of Mars dust, (6) Effect of particle charge on the lung deposition of inhaled medical aerosols, (7) Nanoparticle deposition, and (8) Plasma/Corona discharge processes. A brief discussion on the measurements of charged particles and suggestions for research needs are also included

Physiology and transcriptomics of water-deficit stress responses in wheat cultivars TAM 111 and TAM 112

Citation: Reddy, S. K., Liu, S., Rudd, J. C., Xue, Q., Payton, P., Finlayson, S. A., … Lu, N. (2014). Physiology and transcriptomics of water-deficit stress responses in wheat cultivars TAM 111 and TAM 112. Retrieved from http://krex.ksu.eduHard red winter wheat crops on the U.S. Southern Great Plains often experience moderate to severe drought stress, especially during the grain filling stage, resulting in significant yield losses. Cultivars TAM 111 and TAM 112 are widely cultivated in the region, share parentage and showed superior but distinct adaption mechanisms under water-deficit (WD) conditions. Nevertheless, the physiological and molecular basis of their adaptation remains unknown. A greenhouse study was conducted to understand the differences in the physiological and transcriptomic responses of TAM 111 and TAM 112 to WD stress. Whole-plant data indicated that TAM 112 used more water, produced more biomass and grain yield under WD compared to TAM 111. Leaf-level data at the grain filling stage indicated that TAM 112 had elevated abscisic acid (ABA) content and reduced stomatal conductance and photosynthesis as compared to TAM 111. Sustained WD during the grain filling stage also resulted in greater flag leaf transcriptome changes in TAM 112 than TAM 111. Transcripts associated with photosynthesis, carbohydrate metabolism, phytohormone metabolism, and other dehydration responses were uniquely regulated between cultivars. These results suggested a differential role for ABA in regulating physiological and transcriptomic changes associated with WD stress and potential involvement in the superior adaptation and yield of TAM 112