Insulin-Like Growth Factor-1 (IGF-1) Reduces ischemic changes and increases circulating angiogenic factors in experimentally - induced myocardial infarction in rats

Background: Coronary artery disease is a global health concern in the present day with limited therapies. Extensive efforts have been devoted to find molecular therapies to enhance perfusion and function of the ischemic myocardium. Aim of the present study was to look into the effects of insulin like growth factor -1 (IGF-1) on circulating angiogenic factors after myocardial ischemia in rats.\ud \ud \ud Methods: Adult male Sprague-Dawley rats were randomly divided into 10-days control, myocardial infarction, IGF-1 alone (2 μg/rat/day) and ISO+IGF-1 groups. Isoproterenol (ISO), a synthetic catecholamine was used to induce myocardial infarction. Serum transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF) levels were checked after 10-days of IGF-1 administration.\ud \ud \ud Results: There was a significant increase in heart weight after IGF-1 treatment. A significant increase in cardiac enzyme level (CK-MB and LDH) was seen in isoproterenol treated rats when compared to control group. IGF-1treatment induced a significant increase in serum angiogenic factors, IGF-1, VEGF and TGF beta levels. IGF-1 also reduced the ischemic changes in the myocardium when compared to the isoproterenol alone treated group.\ud \ud \ud Conclusions: In conclusion, treatment with insulin-like growth factor-1 (IGF-1) in myocardial infarction significantly increased circulating angiogenic growth factors like IGF-1, VEGF and TGF beta thus, protecting against myocardial ischemia.\u

USAGE OF INTERACTIVE VIRTUAL REALITY TECHNOLOGY IN PRE-CLINICAL MEDICAL CURRICULUM DELIVERY

Teaching pathology in graduate entry medical education is predominantly through didactic lectures. Other innovative forms of imparting pathology education,  such as learning through virtual microscopy, is necessary in the advancing trend of the medical curriculum. With increasing number of disease processes, some medical universities are now using more state-of-the-art technology driven software.  The ultimate goal of the study was to provide options for students and teachers to use virtual microscope learning modules corresponding to key topics in pathology. Through the pathology sessions in years 1 and 2 in the graduate entry medical curriculum, we developed a series of virtual microscopy sessions. A total of nine pre-clinical modules consisting of 224 respondents were done. The students were invited to take part in an evaluation exercise consisting of basic survey questions. The anonymous data were analyzed qualitatively. A significant number of students responded positively for three important themes: (1) the virtual microscope sessions positively influenced more enthusiasm in learning pathology (84%), (2) both VM and a clinicopathological discussion in the form of case study were necessary to achieve those skills (76%), and (3) the VM sessions led to a sense of personal development as a student (71%). An interactive discussion with the students revealed that they were interested and quite enthusiastic to gain knowledge by this module, which depicted the picture, gross & microscopic with some salient text notes, and they felt that this would also be useful for them in tackling the exams, and in future, during their clinical exposure.&nbsp

Neuroprotective Effects of Alpha Lipoic Acid on Haloperidol-Induced Oxidative Stress in the Rat Brain

Haloperidol is an antipsychotic drug that exerts its' antipsychotic effects by inhibiting dopaminergic neurons. Although the exact pathophysiology of haloperidol extrapyramidal symptoms are not known, the role of reactive oxygen species in inducing oxidative stress has been proposed as one of the mechanisms of prolonged haloperidol-induced neurotoxicity. In the present study, we evaluate the protective effect of alpha lipoic acid against haloperidol-induced oxidative stress in the rat brain. Sprague Dawley rats were divided into control, alpha lipoic acid alone (100 mg/kg p.o for 21 days), haloperidol alone (2 mg/kg i.p for 21 days), and haloperidol with alpha lipoic acid groups (for 21 days). Haloperidol treatment significantly decreased levels of the brain antioxidant enzymes super oxide dismutase and glutathione peroxidase and concurrent treatment with alpha lipoic acid significantly reversed the oxidative effects of haloperidol. Histopathological changes revealed significant haloperidol-induced damage in the cerebral cortex, internal capsule, and substantia nigra. Alpha lipoic acid significantly reduced this damage and there were very little neuronal atrophy. Areas of angiogenesis were also seen in the alpha lipoic acid-treated group. In conclusion, the study proves that alpha lipoic acid treatment significantly reduces haloperidol-induced neuronal damage

Corrigendum to: Fruit Extract Derived from a Mixture of Noni, Pineapple and Mango Capable of Coagulating Milk and Producing Curd with Antidiabetic Activities (Published: Food Technol. Biotechnol. 60 (3) 375-385 (2022) https://doi.org/10.17113/ftb.60.03.22.7456)

The authors request that the Funding section be amended to conform to the correct format required by the funding institution, i.e. the Ministry of Higher Education of Malaysia. Previously written statement in the funding section: Funding for this work was provided by Ministry of Education, Malaysia, with Fundamental Research Grant Scheme (FRGS) with external reference number FRGS/1/2022/STG01/ UMP/02/1 and title: Investigations of Protein from the Lesser Known Tongkat Ali Plants of Stema tuberosa and Polyalthia bullata for Their Potentials in Improving Men’s Health. The present study was the outcome of using chemicals and consumables purchased from this grant without compromising its main objectives and milestones. is changed to: The authors would like to thank Ministry of Higher Education for providing financial support under Fundamental Research Grant Scheme (FRGS) No: FRGS/1/2022/STG01/UMP/02/1 (University reference RDU220110) and Universiti Malaysia Pahang for laboratory facilities. The title of the FRGS grant: Investigations of Protein from the Lesser Known Tongkat Ali Plants of Stema tuberosa and Polyalthia bullata for Their Potentials in Improving Men’s Health. The present study was the outcome of using chemicals and consumables purchased from this grant without compromising its main objectives and milestones

Histopathology and biochemistry analysis of the interaction between sunitinib and paracetamol in mice

Background Sunitinib, a tyrosine kinase inhibitor to treat GIST and mRCC may interact with paracetamol as both undergo P450 mediated biotransformation and P-glycoprotein transport. This study evaluates the effects of sunitinib-paracetamol coadministration on liver and renal function biomarkers and liver, kidney, brain, heart and spleen histopathology. ICR male mice (n = 6 per group/dose) were administered saline (group-A) or paracetamol 500 mg/kg IP (group-B), or sunitinib at 25, 50, 80, 100, 140 mg/kg PO (group-C) or coadministered sunitinib at 25, 50, 80, 100, 140 mg/kg PO and paracetamol IP at fixed dose 500 mg/kg (group-D). Paracetamol was administered 15 min before sunitinib. Mice were sacrificed 4 h post sunitinib administration. Results Group-A serum ALT and AST levels were 14.29 ± 2.31 U/L and 160.37 ± 24.74 U/L respectively and increased to 249.6 ± 222.7 U/L and 377.1 ± 173.6 U/L respectively in group-B; group-C ALT and AST ranged 36.75-75.02 U/L and 204.4-290.3 U/L respectively. After paracetamol coadministration with low sunitinib doses (group-D), ALT and AST concentrations ranged 182.79-221.03 U/L and 259.7-264.4 U/L respectively, lower than group-B. Paracetamol coadministration with high sunitinib doses showed higher ALT and AST values (range 269.6-349.2 U/L and 430.2-540.3 U/L respectively), p 0.05). Mild cardiotoxicity occurred in groups B, C and D. Brain vascular congestion occurred at high doses of sunitinib administered alone or with paracetamol. Hepatic and renal biomarkers correlated with histopathology signs. Conclusions Paracetamol and sunitinib coadministration may lead to dose dependent outcomes exhibiting mild hepatoprotective effect or increased hepatotoxicity. Sunitinib at high doses show renal, cardiac and brain toxicity. Liver and renal function monitoring is recommended.FarmaciaMedicin

The Ranking of Tongkat Ali Plants to Boost Testosterone Hormone Evaluated in both In vitro and In vivo Experiments

In this study, the Tongkat Ali plants were evaluated for effect on testosterone concentrations and their order in boosting the steroidal hormone from the highest to lowest ranked. Eurycoma longifolia(EL), Stema tuberosa(ST), and Polyathia bullata(PB) are collectively referred to as “Tongkat Ali”. The roots were dried and powdered, then extracted with water under reflux. Size-exclusion chromatography was utilized to isolate the protein fraction, which was subsequently characterized using the Bradford Assay and SDS-PAGE. LC-MS was used to test for the presence of natural testosterone within the Tongkat Ali plants. For in vitroand in vivoevaluations, each plant extract was treated with TM-3 Leydig cells (50 μg/mL) for 72 hours and administered in mice (6 mg/mL) twice/day for 20 days. The extraction of EL, ST, and PB yielded 0.74%, 0.46%, and 0.34% w/w of total protein, respectively. SDS-PAGE analysis revealed a single band between 10 and 15 kDa. In vitroevaluations showed that extracts of EL, ST, and PB increased testosterone secretion by 56.02 nmol/L (41.1% compared to the untreated controls), 40.49 nmol/L (18.65%) and 36.99 nmol/L (10.93%), respectively. In the in vivostudies, EL extract showed the highest testosterone concentration at 3.85 nmol/L (51.18% compared to the untreated controls), followed by ST with 3.35 nmol/L (43.95%) and lastly, PB at only 1.88 nmol/L (9.1%). Tongkat Ali plants boosted the male hormones in both in vitroand in vivostudies, with the order being EL>ST>PB

Glycoproteins Isolated from Tongkat Ali Plants Capable to elevate Testosterone in both in vitro and in vivo Experiments

Tongkat Ali, a common name used to identify at least three plants, Eurycoma longifolia, Polyathia bullata, and Stema tuberosa, is recognized for its aphrodisiac bioactive constituent attributed to glycoproteins, particularly in E. longifolia. This study explores the presence and bioactivity of glycoproteins in P. bullata and S. tuberosa, employing similar extraction methods to E. longifolia. The crude extracts from all three plants were isolated, and glycoproteins were separated using lectin affinity chromatography. The glycoprotein yields were determined, and the protein characterization was conducted using Sodium Dodecyl Sulphate Gel Electrophoresis (SDS PAGE). Consequently, through both in vitro and in vivo experiments, the bioactivity of the isolated glycoproteins was tested for their ability to elevate testosterone levels. In vitro studies conducted on approximately 4000 TM-3 Leydig cells revealed that treatment with 50 µg/mL of glycoproteins extracted from P. bullata and S. tuberosa resulted in a notable increase in testosterone concentration by 45% and 48%, respectively, compared to the untreated cells after 72 h. Moreover, in groups of mice administered with the isolated glycoproteins for 20 days, testosterone concentrations increased by over 50% compared to the control groups. Despite the relatively low yields of glycoproteins, with only 0.26% and 0.21% present in the roots of P. bullata and S. tuberosa, respectively, their effectiveness was evident. This highlights that the aphrodisiac bioactive constituents, are glycoproteins, within all three Tongkat Ali plants

In Vitro and In Vivo evaluations of Smilax myosotiflora (Ubi Jaga) compared to the well-known aphrodisiac plant of Eurycoma longifolia (Tongkat Ali)

Smilax myosotiflora and Eurycoma longifolia are recognized as aphrodisiac medicinal plants in Malaysia. E. longifolia is widely purchased and extensively studied for its aphrodisiac effects, unlike S. myosotiflora. There has been some uncertainty regarding whether S. myosotiflora is comparable to E. longifolia in its aphrodisiac properties. In this study, both plants were extracted using a water distillation method under reflux, followed by clarification, freeze-drying, and yield comparison. The extracts were then subjected to in vitro experiments using TM-3 Leydig cell cultures. In vivo experiments involving mice were administered oral doses of 6 mg of each extract twice daily for 20 days. The results indicated that the extraction yield for E. longifolia was higher at 7.43% (w/w) compared to 5.06% (w/w) for S. myosotiflora. In the in vitro experiments, Leydig testicular cells treated with 50 μg/mL of E. longifolia for 72 hours exhibited an 83.93% increase in testosterone concentrations compared to untreated controls. Additionally, testosterone levels increased by 94.61% in mice treated with E. longifolia compared to control mice. However, no increase in testosterone was reported in either the in vitro or in vivo experiments conducted with S. myosotiflora. Both S. myosotiflora and E. longifolia were confirmed to enhance libido, as indicated by increased frequencies of mounting and intromission in pairing experiments with treated males and females. In conclusion, while both plants can enhance libido, S. myosotiflora did not demonstrate the ability to boost testosterone during the short duration of the treatments

Transdermal delivery of tolterodine tartrate for overactive bladder treatment: In vitro, and in vivo evaluation

The purpose of the study was to develop a transdermal tolterodine tartrate (TT) patch and to analyse its efficacy for overactive bladder (OAB) treatment. Patches were prepared using various polymers and plasticizers via the solvent casting method. The patches were characterized for tensile strength, thickness, moisture content, modulus of elasticity and water absorption capacity. Differential scanning calorimetry and Fourier transform infrared analyses were also performed. To determine patch effectiveness, in vitro release, permeation and animal studies were performed. The patches showed satisfactory percentage of release, up to 89.9 %, and their mechanical properties included thickness (0.10–0.15 mm), tensile strength (4.62–9.98 MPa) and modulus of elasticity (20–29 MPa). There were no significant interactions between TT and other excipients. Animal studies indicated that the TT patch reduced the incidence of side effects; however, studies of longer duration are required to determine the effectiveness in treating OAB