161 research outputs found
Παρατηρήσεις με βάση τη μετάφραση του πρώτου κεφαλαίου του Καπετάν Μιχάλη του Νίκου Καζαντζάκη
夏期発表
Monitoramento de processos automatizados utilizando rpa
TCC (graduação) - Universidade Federal de Santa Catarina. Campus Blumenau. Engenharia de Controle e AutomaçãoAtualmente, nas empresas, há vários processos de negócios que geram uma grande
quantidade de dados para serem manipulados e processados repetidamente. Geralmente,
esses processos são realizados de maneira manual por funcionários que utilizam um com putador. Porém essa metodologia de trabalho tornou-se pouco eficiente, e até mesmo
pode causar problemas ergonômicos no funcionário em virtude de movimentos repetiti vos. Visando melhorar esse cenário e o desempenho nos processos, surgiu a automação de
processos robóticos (RPA - Robotic Process Automation). RPA é uma tecnologia capaz
de executar processos repetitivos e massivos de forma automatizada utilizando um soft ware de computador. No entanto, os processos robóticos de RPA estão sujeitos a falhas
e, por isso, é necessário monitorar o seu funcionamento. Enquanto este monitoramento é
usualmente realizado por um operador humano, considera-se adequado torná-lo também
automatizado. Assim, nesse trabalho, foi desenvolvido uma aplicação de automação RPA
para realizar o processo de monitoramento de processos automatizados de forma auto mática. Basicamente a solução de automação desenvolvida identifica erros ou falhas que
ocorreram nos processos automatizados e informa às partes interessadas. Este processo,
anteriormente, era realizado de forma manual. Os resultados obtidos se mostraram satis fatórios, pois com a implementação da solução desenvolvida houve uma redução de 80%
no tempo de execução, gerando redução de custo e aumento da eficiência
Decreased expression of prenyl diphosphate synthase subunit 2 correlates with reduced survival of patients with gastric cancer
Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus
Genetic research on fibrosis outset and its progression in chronic hepatitis (CH) by hepatitis C virus (HCV) are limited. The lack of cytogenetic data led us to investigate the presence of micronuclei (MNi), as a sign of genomic damage. Hepatocytes of hepatic parenchyma from 62 cases diagnosed with CH associated with HCV and displaying different degrees of fibrosis (F1-F4) were analyzed. These data were compared to 15 cases without fibrosis (F0). Twelve healthy liver parenchyma samples were included as control. All samples were obtained from paraffin-embedded archival material. Micronucleated hepatocytes (MN-Heps) were analyzed through Feulgen/Fast-green staining. Results showed that the rates of MN-Heps in the F4 group were statistically significant (p < 0.05) and higher than those in the control group. Like results were also obtained on comparing F4 with F0, F1, F2 and F3 cases. Conversely, differences were not significant (p > 0.05) on comparing F0, F1, F2, F3, one against the other, as well as individual versus control. Although chromosomal losses in CH were detected, it was shown that liver parenchyma with fibrosis in the initial stages (F1-F3) cannot be considered cytogenetically abnormal
Influence of Substrates on the Surface Characteristics and Membrane Proteome of Fibrobacter succinogenes S85
Although Fibrobacter succinogenes S85 is one of the most proficient cellulose degrading bacteria among all mesophilic organisms in the rumen of herbivores, the molecular mechanism behind cellulose degradation by this bacterium is not fully elucidated. Previous studies have indicated that cell surface proteins might play a role in adhesion to and subsequent degradation of cellulose in this bacterium. It has also been suggested that cellulose degradation machinery on the surface may be selectively expressed in response to the presence of cellulose. Based on the genome sequence, several models of cellulose degradation have been suggested. The aim of this study is to evaluate the role of the cell envelope proteins in adhesion to cellulose and to gain a better understanding of the subsequent cellulose degradation mechanism in this bacterium. Comparative analysis of the surface (exposed outer membrane) chemistry of the cells grown in glucose, acid-swollen cellulose and microcrystalline cellulose using physico-chemical characterisation techniques such as electrophoretic mobility analysis, microbial adhesion to hydrocarbons assay and Fourier transform infra-red spectroscopy, suggest that adhesion to cellulose is a consequence of an increase in protein display and a concomitant reduction in the cell surface polysaccharides in the presence of cellulose. In order to gain further understanding of the molecular mechanism of cellulose degradation in this bacterium, the cell envelope-associated proteins were enriched using affinity purification and identified by tandem mass spectrometry. In total, 185 cell envelope-associated proteins were confidently identified. Of these, 25 proteins are predicted to be involved in cellulose adhesion and degradation, and 43 proteins are involved in solute transport and energy generation. Our results supports the model that cellulose degradation in F. succinogenes occurs at the outer membrane with active transport of cellodextrins across for further metabolism of cellodextrins to glucose in the periplasmic space and inner cytoplasmic membrane
Bacterial Surface Appendages Strongly Impact Nanomechanical and Electrokinetic Properties of Escherichia coli Cells Subjected to Osmotic Stress
The physicochemical properties and dynamics of bacterial envelope, play a major role in bacterial activity. In this study, the morphological, nanomechanical and electrohydrodynamic properties of Escherichia coli K-12 mutant cells were thoroughly investigated as a function of bulk medium ionic strength using atomic force microscopy (AFM) and electrokinetics (electrophoresis). Bacteria were differing according to genetic alterations controlling the production of different surface appendages (short and rigid Ag43 adhesins, longer and more flexible type 1 fimbriae and F pilus). From the analysis of the spatially resolved force curves, it is shown that cells elasticity and turgor pressure are not only depending on bulk salt concentration but also on the presence/absence and nature of surface appendage. In 1 mM KNO3, cells without appendages or cells surrounded by Ag43 exhibit large Young moduli and turgor pressures (∼700–900 kPa and ∼100–300 kPa respectively). Under similar ionic strength condition, a dramatic ∼50% to ∼70% decrease of these nanomechanical parameters was evidenced for cells with appendages. Qualitatively, such dependence of nanomechanical behavior on surface organization remains when increasing medium salt content to 100 mM, even though, quantitatively, differences are marked to a much smaller extent. Additionally, for a given surface appendage, the magnitude of the nanomechanical parameters decreases significantly when increasing bulk salt concentration. This effect is ascribed to a bacterial exoosmotic water loss resulting in a combined contraction of bacterial cytoplasm together with an electrostatically-driven shrinkage of the surface appendages. The former process is demonstrated upon AFM analysis, while the latter, inaccessible upon AFM imaging, is inferred from electrophoretic data interpreted according to advanced soft particle electrokinetic theory. Altogether, AFM and electrokinetic results clearly demonstrate the intimate relationship between structure/flexibility and charge of bacterial envelope and propensity of bacterium and surface appendages to contract under hypertonic conditions
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