Development of resin canals during somatic embryogenesis in callus cultures of <i style="">Commiphora wightii</i>

267-270Formation of resin canal in somatic embryos of Commiphora wightii (Arnott.) Bhandari has been reported. Resin canal formation is a prerequisite for obtaining resin canal based secondary metabolites and resin canal formation in callus cultures is not known. Resin canals were observed in torpedo shaped and cotyledonary stage embryos. Early stages were devoid of it. Resin canals formed in somatic embryos were comparable with those formed in the stem. This provides large quantities of aseptic resin canals in organized structures, which can be used for the production of guggulsterones through biotechnological methods relieving pressure on natural resources

<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language:EN-IN; mso-bidi-language:HI" lang="EN-IN">Establishment of embryonic cultures and somatic embryogenesis in callus culture of <i>guggul-Commiphora wightii</i> (Arnott.) Bhandari</span>

69-77<span style="font-size:12.0pt;mso-bidi-font-size: 14.0pt;line-height:115%;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-ansi-language:en-in;mso-fareast-language:en-in;="" mso-bidi-language:hi"="" lang="EN-IN">Somatic embryogenesis in callus cultures of Commiphora wigitii (Arnott.) Bhandari was achieved. Though the frequency of explants producing embryonic culture was low, immature zygotic embryos were the only suitable explants to produce embryonic callus after reciprocal transfers on media containing 2,4,5-trichlorophenoxy acetic acid (0. 1 mgl-1) and kinetin(0.1mgl-1 ) or devoid of growth regulators. All other media failed to produce embryonic callus. Embryonic cells were small, densely filled with cytoplasm and isodiametric as compared to non-embryonic cells, which were large, elongated and vacuolated. Maximum growth of embryonic callus was recorded on modified MS medium (MS-2 medium) supplemented with BA (0.25 mgl-1) and IBA (0.1 mgl-1). MS-2 salts supported higher growth of callus as compared to tissues grown on B5 medium containing same concentrations of plant growth regulators. Exogenous medium nutrients had no effect on somatic embryo development whereas plant growth regulators had little effect. Asynchronously growing embryos formed plantlets regularly which were successfully transferred to the field conditions.</span