Comparison of Reasoner’s 2A Agar and Muller Hinton Agar Media for Microbiological Monitoring of Dialysis Water

Background and Aim: Microbiological culture of dialysis water is a routine safety measure. In, Khorramabad laboratories perform these cultures on Muller Hinton Agar (MHA) at 35–378C for 48 h, not on the Reasoner’s 2A agar (R2A agar) at 17–238ºC for 7 days recommended by international standards, the objective of the present study was the comparison of the efficiency of R2A and MHA media in the counting of heterotrophic bacteria in the samples of water collected in dialysis centers from 2 hospitals in Khorramabad, from September to November 2019. Methods: A total of 165 samples of treated water in dialysis centers were collected aseptically and then transported in ice‑packs to the Department of Medical Microbiology of the Lorestan University of Medical Sciences and the pour plate technique was carried out for the enumerating of heterotrophic bacteria. Finally, bacterial colonies were counted after incubation at 34±2ºC for 48 hours on MHA and 25ºC for 1 week on R2A. Results: Results showed heterotrophic bacterial counts in R2A were greater than those in MHA in 89% of the samples, so enumeration of heterotrophic bacteria should be carried out in R2A agar associated with longer incubation times, because of the greater sensitivity. The proportion of water samples yielding colony counts ≥200 CFU/mL by R2A -7d was significantly different from the proportion by MHA-48h (p<0.001). Conclusion: The results proposed using R2A agar combined with relative low culture temperature (20-25°C), and an extended incubation time (7-10 days) is more efficient. However, as the spectrum of bacterial contamination is not similar for dialysis centers and countries, many studies using different media and culture parameters are required to confirm this. *Corresponding Author: Faranak Rezaei; Email: [email protected] Please cite this article as: Pouladi I, Delfani S, Hadian B, Soroush S, Anbari K, Rezaei F. Comparison of Reasoner’s 2A Agar and Muller Hinton Agar Media for Microbiological Monitoring of Dialysis Water. Arch Med Lab Sci. 2020;6:1-5 (e10). https://doi.org/10.22037/amls.v6.3290

Investigation of the Potential Presence of Porphyromonas gingivalis in Esophageal Squamous Cell Carcinoma (ESCC) Paraffin Embedded Tissue Samples

Background and Aim: Esophageal cancer is the eighth most common cancer and the sixth leading cause of cancer death worldwide Evidence suggests that there is a link between bacterial infection and malignancy. There are few studies on the prevalence of Porphyromonas gingivalis in esophageal squamous cell carcinoma (ESCC), so this study aimed to investigate the possible presence of this bacterium in ESCC tissue samples.Materials and Methods: In this study, 34 esophageal squamous cell carcinoma samples were collected to evaluate the potential presence of Porphyromonas gingivalis. After extracting the DNA, the polymerase chain reaction (PCR) technique was used to detect the presence of the bacterium molecularly.Results: The age range of the study population was 26 to 90 years, with a mean age of 63 years. Most tissue samples come from stage I cancer (73.5%). Based on the molecular analysis, no P. gingivalis was detected in any biopsy specimensConclusion: P. gingivalis infection and ESCC were not correlated based on the current in this study. Likely, the use of fresh samples, more accurate diagnostic methods, geographic differences, and larger sample sizes all contribute to the differences in results between related research, which can be clarified through large-scale studies

Investigation of the Potential Presence of Porphyromonas gingivalis in Esophageal Squamous Cell Carcinoma (ESCC) Paraffin Embedded Tissue Samples

Background and Aim: Esophageal cancer is the eighth most common cancer and the sixth leading cause of cancer death worldwide Evidence suggests that there is a link between bacterial infection and malignancy. There are few studies on the prevalence of Porphyromonas gingivalis in esophageal squamous cell carcinoma (ESCC), so this study aimed to investigate the possible presence of this bacterium in ESCC tissue samples. Materials and Methods: In this study, 34 esophageal squamous cell carcinoma samples were collected to evaluate the potential presence of Porphyromonas gingivalis. After extracting the DNA, the polymerase chain reaction (PCR) technique was used to detect the presence of the bacterium molecularly. Results: The age range of the study population was 26 to 90 years, with a mean age of 63 years. Most tissue samples come from stage I cancer (73.5%). Based on the molecular analysis, no P. gingivalis was detected in any biopsy specimens Conclusion: P. gingivalis infection and ESCC were not correlated based on the current in this study. Likely, the use of fresh samples, more accurate diagnostic methods, geographic differences, and larger sample sizes all contribute to the differences in results between related research, which can be clarified through large-scale studies

Cytotoxic Effect and Cell Death Mechanism of Salmonella Typhimurium Protein Fractions on Breast Cancer Cells In Vitro

Background and purpose: Cancer is the second leading cause of death in the world after cardiovascular disease. In relation to cancerous diseases in developed countries, the prevalence of breast cancer is increasing. Treatment in early stages is of great importance in the recovery of patients. The purpose of this study was to investigate the cytotoxic effect of bacterial fractions of Salmonella typhimurium on growth and proliferation of breast cancer cells in vitro. Materials and methods: In this experimental study, the breast cancer cell line MCF7 was used. Different bacterial fractions were prepared by ammonium sulfate method. Interactions between cancer cells and different concentrations of Salmonella typhimurium fractions were studied at 24 and 48 hours. Cell proliferation was assessed by MTT assay. Results: According to MTT test, bacterial fractions and 80% protein deposition at 24 and 48 hours showed the highest cytotoxicity effect at 24 hours and in 100 μg/ml bacterial lysates and 40 μg/ml of 80% protein deposition. Following the treatment of cancer cells with bacterial lysate, the rate of induction of apoptosis was 67% and necrosis was 7%. Conclusion: Bacterial fractions of Salmonella typhimurium have high toxicity and lethal effect on breast cancer cells and induce apoptosis. Purification of these compounds could reveal new information on the mechanisms of cell death and cell signaling that could be suggested in the future as a basis to develop alternative cancer treatments