Advancing Transparency and Impact of Research: Initiating Crosstalk between Indigenous Research and Mainstream “Open Science”

To answer questions about human psychology, psychological science needs to yield credible findings. Because of their goals of understanding people’s lived experiences and advocating for the needs of the Native communities, Indigenous scholars tend to use community-based participatory research (CBPR) or approach science from a constructivist framework. The primary goal of mainstream psychological science is to uncover generalizable facts about human functioning. Approached from a postpositivist framework, mainstream psychological scholars tend to assume the possibility of identifying researcher biases and achieving objective science. Recently, many psychological findings failed to replicate in new samples. The replication crisis has raised concerns about the validity of psychological science. Mainstream open science practices have been promoted as a solution to the replication crisis; the movement encourages researchers to emphasize transparency and accountability to the broad scientific community. The notion of transparency aligns with the principles of CBPR—approach common in Indigenous research. Yet, open science practices are not widely adopted in Indigenous research, and mainstream open science does not emphasize researchers’ accountability to the communities that their science is intended to serve. We examined Indigenous researchers’ awareness and concerns about mainstream open science. Participants endorsed the value of being transparent in the knowledge-production process with the participants and their communities. They also were concerned about being disadvantaged, and the possible negative impact of data sharing on the Native communities. We suggest that there is value in connecting mainstream open science and Indigenous research to advance science that empowers people and makes positive community impact

Animal Agriculture in South Carolina: A Fact Book

Livestock Production/Industries,

Virus Identification in Unknown Tropical Febrile Illness Cases Using Deep Sequencing

Dengue virus is an emerging infectious agent that infects an estimated 50–100 million people annually worldwide, yet current diagnostic practices cannot detect an etiologic pathogen in ∼40% of dengue-like illnesses. Metagenomic approaches to pathogen detection, such as viral microarrays and deep sequencing, are promising tools to address emerging and non-diagnosable disease challenges. In this study, we used the Virochip microarray and deep sequencing to characterize the spectrum of viruses present in human sera from 123 Nicaraguan patients presenting with dengue-like symptoms but testing negative for dengue virus. We utilized a barcoding strategy to simultaneously deep sequence multiple serum specimens, generating on average over 1 million reads per sample. We then implemented a stepwise bioinformatic filtering pipeline to remove the majority of human and low-quality sequences to improve the speed and accuracy of subsequent unbiased database searches. By deep sequencing, we were able to detect virus sequence in 37% (45/123) of previously negative cases. These included 13 cases with Human Herpesvirus 6 sequences. Other samples contained sequences with similarity to sequences from viruses in the Herpesviridae, Flaviviridae, Circoviridae, Anelloviridae, Asfarviridae, and Parvoviridae families. In some cases, the putative viral sequences were virtually identical to known viruses, and in others they diverged, suggesting that they may derive from novel viruses. These results demonstrate the utility of unbiased metagenomic approaches in the detection of known and divergent viruses in the study of tropical febrile illness

Effects of fluoxetine on functional outcomes after acute stroke (FOCUS): a pragmatic, double-blind, randomised, controlled trial

Background Results of small trials indicate that fluoxetine might improve functional outcomes after stroke. The FOCUS trial aimed to provide a precise estimate of these effects. Methods FOCUS was a pragmatic, multicentre, parallel group, double-blind, randomised, placebo-controlled trial done at 103 hospitals in the UK. Patients were eligible if they were aged 18 years or older, had a clinical stroke diagnosis, were enrolled and randomly assigned between 2 days and 15 days after onset, and had focal neurological deficits. Patients were randomly allocated fluoxetine 20 mg or matching placebo orally once daily for 6 months via a web-based system by use of a minimisation algorithm. The primary outcome was functional status, measured with the modified Rankin Scale (mRS), at 6 months. Patients, carers, health-care staff, and the trial team were masked to treatment allocation. Functional status was assessed at 6 months and 12 months after randomisation. Patients were analysed according to their treatment allocation. This trial is registered with the ISRCTN registry, number ISRCTN83290762. Findings Between Sept 10, 2012, and March 31, 2017, 3127 patients were recruited. 1564 patients were allocated fluoxetine and 1563 allocated placebo. mRS data at 6 months were available for 1553 (99·3%) patients in each treatment group. The distribution across mRS categories at 6 months was similar in the fluoxetine and placebo groups (common odds ratio adjusted for minimisation variables 0·951 [95% CI 0·839–1·079]; p=0·439). Patients allocated fluoxetine were less likely than those allocated placebo to develop new depression by 6 months (210 [13·43%] patients vs 269 [17·21%]; difference 3·78% [95% CI 1·26–6·30]; p=0·0033), but they had more bone fractures (45 [2·88%] vs 23 [1·47%]; difference 1·41% [95% CI 0·38–2·43]; p=0·0070). There were no significant differences in any other event at 6 or 12 months. Interpretation Fluoxetine 20 mg given daily for 6 months after acute stroke does not seem to improve functional outcomes. Although the treatment reduced the occurrence of depression, it increased the frequency of bone fractures. These results do not support the routine use of fluoxetine either for the prevention of post-stroke depression or to promote recovery of function. Funding UK Stroke Association and NIHR Health Technology Assessment Programme

Advancing Transparency and Impact of Research: Initiating Crosstalk between Indigenous Research and Mainstream “Open Science”

To answer questions about human psychology, psychological science needs to yield credible findings. Because of their goals of understanding people’s lived experiences and advocating for the needs of the Native communities, Indigenous scholars tend to use community-based participatory research (CBPR) or approach science from a constructivist framework. The primary goal of mainstream psychological science is to uncover generalizable facts about human functioning. Approached from a postpositivist framework, mainstream psychological scholars tend to assume the possibility of identifying researcher biases and achieving objective science. Recently, many psychological findings fail to replicate in new samples. The replication crisis raised concerns about the validity of psychological science. The mainstream open science has been promoted as a solution to this replication crisis; the open science movement encourages researchers to emphasize transparency and accountability to the broad research community. The notion of transparency aligns with the principles of CBPR—research approach common in Indigenous research. Yet, open science practices are not widely adopted in Indigenous research, and mainstream open science does not emphasize researchers’ accountability to the communities that their science is intended to serve. We examined Indigenous researchers’ awareness and concerns about mainstream open science. Participants endorsed the value of transparency with the participants and their communities. They also were concerned about being disadvantaged and the possible negative impact of data sharing on the Native communities. We suggest that there is value in connecting mainstream open science and Indigenous research to advance science that empowers people and makes positive community impact