Comprehensive Life Cycle Environmental Assessment of Preventive Maintenance Techniques for Asphalt Pavement

Preventive maintenance (PM) is regarded as the most economical maintenance strategy for asphalt pavement, but the life cycle environmental impacts (LCEI) of different PM techniques have not yet been comprehensively assessed and compared, thus hindering sustainable PM planning. This study aims to comprehensively estimate and compared the LCEI of five PM techniques then propose measures to reduce environmental impacts in PM design by using life cycle assessment (LCA), including fog seal with sand, micro-surfacing, composite seal, ultra-thin asphalt overlay, and thin asphalt overlay. Afterwards, ten kinds of LCEI categories and energy consumption of PM techniques were compared from the LCA phases, and inventory inputs perspectives, respectively. Results show that fog seal with sand and micro-surfacing can lower all LCEI scores by more than 50%. The environmental performance of five PM techniques provided by sensitivity analysis indicated that service life may not create significant impact on LCA results to some extent. Moreover, four PM combination plans were developed and compared for environmental performance, and results show that the PM plan only includes seal coat techniques that can reduce the total LCEI by 7–29% in pavement life. Increasing the frequency of seal coat techniques can make the PM plans more sustainable

Using Simulations to Understand the Reading of Rapidly Displayed Subtitles

Liao et al. (2020) reported an eye-movement experiment in which subtitles were displayed at three different rates, with a key finding being that, with increasing speeds, participants made fewer, shorter fixations and longer saccades. To understand why these eye-movement behaviors might be adaptive, we completed simulations using the E-Z Reader model (Reichle et al., 2012) to examine how subtitle speed might affect word identification and sentence comprehension, as well as the efficacy of six possible compensatory reading strategies. These simulations suggest that the imposition of a lexical-processing deadline and/or strategy of skipping short words may support reading comprehension in impoverished conditions

Manipulating Orbital Angular Momentum Entanglement in Three-Dimensional Spiral Nonlinear Photonic Crystals

We propose and theoretically investigate two-photon orbital angular momentum (OAM) correlation through spontaneous parameter down-conversion (SPDC) processes in three-dimensional (3D) spiral nonlinear photonic crystals (NPCs). By properly designing the NPC structure, one can feasibly modulate the OAM-correlated photon pair, which provides a potential platform to realize high-dimensional entanglement for quantum information processing and quantum communications

Assessment of Modulus Attenuation of Cement and Lime-Fly Ash Semi-Rigid Road Base Materials

For asphalt pavement structures, semi-rigid road base course has to sustain repeated high-axle load during its service life and the performance of semi-rigid road base materials directly influences the durability of pavement structures. The dynamic compressive resilience modulus of two commonly used semi-rigid road base materials, cement stabilized aggregates (CSG) and lime-fly ash stabilized aggregates (LFSG) were evaluated at different frequencies using a Universal Testing Machine (UTM). The results showed that LFSG had higher dynamic modulus than that of CSG and the load frequency had less influence on the dynamic modulus of these two semi-rigid road base materials. The four-point bending test was applied to measure the flexural-bending strength and the fatigue life of these two semi-rigid materials. A higher flexural-bending strength of LFSG indicated its better bearing capacity than that of CSG. The fatigue life of LFSG and CSG decreased with the increase of stress–strength ratio and the LFSG performed better in terms of fatigue resistance. The fatigue damage models of CSG and LFSG based on Stress-Life (S-N) curve are established. As per incremental-recursive mechanics, a general modulus degradation model was established and verified by the results of full-scale accelerate loading test. This model cannot only be used to predict the fatigue deterioration of semi-rigid road base materials under different stress levels, but is also able to calculate the current bending elastic modulus based on its initial modulus value

Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines

Stem rust is one of the most destructive diseases of wheat worldwide. The recent emergence of wheat stem rust race Ug99 (TTKS based on the North American stem rust race nomenclature system) and related strains threaten global wheat production because they overcome widely used genes that had been effective for many years. Host resistance is likely to be more durable when several stem rust resistance genes are pyramided in a single wheat variety; however, little is known about the resistance genotypes of widely used wheat germplasm. In this study, a diverse collection of wheat germplasm was haplotyped for stem rust resistance genes Sr2, Sr22, Sr24, Sr25, Sr26, Sr36, Sr40, and 1A.1R using linked microsatellite or simple sequence repeat (SSR) and sequence tagged site (STS) markers. Haplotype analysis indicated that 83 out of 115 current wheat breeding lines from the International Maize and Wheat Improvement Center (CIMMYT) likely carry Sr2. Among those, five out of 94 CIMMYT spring lines tested had both Sr2 and Sr25 haplotypes. Five out of 22 Agriculture Research Service (ARS) lines likely have Sr2 and a few have Sr24, Sr36, and 1A.1R. Two out of 43 Chinese accessions have Sr2. No line was found to have the Sr26 and Sr40 haplotypes in this panel of accessions. DArT genotyping was used to identify new markers associated with the major stem resistance genes. Four DArT markers were significantly associated with Sr2 and one with Sr25. Principal component analysis grouped wheat lines from similar origins. Almost all CIMMYT spring wheats were clustered together as a large group and separated from the winter wheats. The results provide useful information for stem rust resistance breeding and pyramiding

Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines

Stem rust is one of the most destructive diseases of wheat worldwide. The recent emergence of wheat stem rust race Ug99 (TTKS based on the North American stem rust race nomenclature system) and related strains threaten global wheat production because they overcome widely used genes that had been effective for many years. Host resistance is likely to be more durable when several stem rust resistance genes are pyramided in a single wheat variety; however, little is known about the resistance genotypes of widely used wheat germplasm. In this study, a diverse collection of wheat germplasm was haplotyped for stem rust resistance genes Sr2, Sr22, Sr24, Sr25, Sr26, Sr36, Sr40, and 1A.1R using linked microsatellite or simple sequence repeat (SSR) and sequence tagged site (STS) markers. Haplotype analysis indicated that 83 out of 115 current wheat breeding lines from the International Maize and Wheat Improvement Center (CIMMYT) likely carry Sr2. Among those, five out of 94 CIMMYT spring lines tested had both Sr2 and Sr25 haplotypes. Five out of 22 Agriculture Research Service (ARS) lines likely have Sr2 and a few have Sr24, Sr36, and 1A.1R. Two out of 43 Chinese accessions have Sr2. No line was found to have the Sr26 and Sr40 haplotypes in this panel of accessions. DArT genotyping was used to identify new markers associated with the major stem resistance genes. Four DArT markers were significantly associated with Sr2 and one with Sr25. Principal component analysis grouped wheat lines from similar origins. Almost all CIMMYT spring wheats were clustered together as a large group and separated from the winter wheats. The results provide useful information for stem rust resistance breeding and pyramiding

Table_2_Oral delivery of Eimeria acervulina transfected sequentially with two copies of the VP2 gene induces immunity against infectious bursal disease virus in chickens.DOCX

Chicken coccidiosis caused by Eimeria spp. can occur on almost all poultry farms, causing huge economic losses to the industry. Genetically manipulated Eimeria parasites as a vaccine vector to deliver viral antigens have been reported. In our preliminary study, transgenic E. acervulina expressing a VP2 gene (Ea-VP2) of the infectious bursal disease virus (IBDV) demonstrated partial protection against IBDV infection. To enhance immune responses, we aimed to increase the VP2 gene copy number in transgenic E. acervulina. In this study, we used a novel plasmid vector carrying a VP2 gene fused with three flag tags and a red fluorescent reporter gene (mCherry). The vector was introduced into Ea-VP2 sporozoites through nucleofection, leading to the generation of Ea-2VP2. Subsequent analysis revealed a notable escalation in the fluorescent rate, increasing from 0.11 to 95.1% following four consecutive passages facilitated by fluorescent-activated cell sorting. Verification via PCR, Western blot, and immunofluorescence confirmed the successful construction of the Ea-2VP2 population. Despite lower fecundity compared to wild-type E. acervulina, Ea-2VP2 maintained immunogenicity. Our research effectively created a transgenic E. acervulina strain transfected sequentially with two copies of the VP2 gene from IBDV. This modification resulted in an increased humoral immune response after primary immunization in chickens. Additionally, it demonstrated a degree of protection within the bursa against IBDV infection. Future studies will focus on further enhancing immune response levels.</p

Image_1_Oral delivery of Eimeria acervulina transfected sequentially with two copies of the VP2 gene induces immunity against infectious bursal disease virus in chickens.TIF

Chicken coccidiosis caused by Eimeria spp. can occur on almost all poultry farms, causing huge economic losses to the industry. Genetically manipulated Eimeria parasites as a vaccine vector to deliver viral antigens have been reported. In our preliminary study, transgenic E. acervulina expressing a VP2 gene (Ea-VP2) of the infectious bursal disease virus (IBDV) demonstrated partial protection against IBDV infection. To enhance immune responses, we aimed to increase the VP2 gene copy number in transgenic E. acervulina. In this study, we used a novel plasmid vector carrying a VP2 gene fused with three flag tags and a red fluorescent reporter gene (mCherry). The vector was introduced into Ea-VP2 sporozoites through nucleofection, leading to the generation of Ea-2VP2. Subsequent analysis revealed a notable escalation in the fluorescent rate, increasing from 0.11 to 95.1% following four consecutive passages facilitated by fluorescent-activated cell sorting. Verification via PCR, Western blot, and immunofluorescence confirmed the successful construction of the Ea-2VP2 population. Despite lower fecundity compared to wild-type E. acervulina, Ea-2VP2 maintained immunogenicity. Our research effectively created a transgenic E. acervulina strain transfected sequentially with two copies of the VP2 gene from IBDV. This modification resulted in an increased humoral immune response after primary immunization in chickens. Additionally, it demonstrated a degree of protection within the bursa against IBDV infection. Future studies will focus on further enhancing immune response levels.</p

Table_1_Oral delivery of Eimeria acervulina transfected sequentially with two copies of the VP2 gene induces immunity against infectious bursal disease virus in chickens.DOCX

Chicken coccidiosis caused by Eimeria spp. can occur on almost all poultry farms, causing huge economic losses to the industry. Genetically manipulated Eimeria parasites as a vaccine vector to deliver viral antigens have been reported. In our preliminary study, transgenic E. acervulina expressing a VP2 gene (Ea-VP2) of the infectious bursal disease virus (IBDV) demonstrated partial protection against IBDV infection. To enhance immune responses, we aimed to increase the VP2 gene copy number in transgenic E. acervulina. In this study, we used a novel plasmid vector carrying a VP2 gene fused with three flag tags and a red fluorescent reporter gene (mCherry). The vector was introduced into Ea-VP2 sporozoites through nucleofection, leading to the generation of Ea-2VP2. Subsequent analysis revealed a notable escalation in the fluorescent rate, increasing from 0.11 to 95.1% following four consecutive passages facilitated by fluorescent-activated cell sorting. Verification via PCR, Western blot, and immunofluorescence confirmed the successful construction of the Ea-2VP2 population. Despite lower fecundity compared to wild-type E. acervulina, Ea-2VP2 maintained immunogenicity. Our research effectively created a transgenic E. acervulina strain transfected sequentially with two copies of the VP2 gene from IBDV. This modification resulted in an increased humoral immune response after primary immunization in chickens. Additionally, it demonstrated a degree of protection within the bursa against IBDV infection. Future studies will focus on further enhancing immune response levels.</p