1 research outputs found
Profiling the Serum Albumin Cys34 Adductome of Solid Fuel Users in Xuanwei and Fuyuan, China
Xuanwei
and Fuyuan counties in China have the highest lung cancer rates in
the world due to household air pollution from combustion of smoky
coal for cooking and heating. To discover potential biomarkers of
indoor combustion products, we profiled adducts at the Cys34 locus
of human serum albumin (HSA) in 29 nonsmoking Xuanwei and Fuyuan females
who used smoky coal, smokeless coal, or wood and 10 local controls
who used electricity or gas fuel. Our untargeted “adductomics”
method detected 50 tryptic peptides of HSA, containing Cys34 and prominent
post-translational modifications. Putative adducts included Cys34
oxidation products, mixed disulfides, rearrangements, and truncations.
The most significant differences in adduct levels across fuel types
were observed for <i>S</i>-glutathione (<i>S</i>-GSH) and <i>S</i>-γ-glutamylcysteine (<i>S</i>-γ-GluCys), both of which were present at lower levels in subjects
exposed to combustion products than in controls. After adjustment
for age and personal measurements of airborne benzo(<i>a</i>)pyrene, the largest reductions in levels of <i>S</i>-GSH
and <i>S</i>-γ-GluCys relative to controls were observed
for users of smoky coal, compared to users of smokeless coal and wood.
These results point to possible depletion of GSH, an essential antioxidant,
and its precursor γ-GluCys in nonsmoking females exposed to
indoor-combustion products in Xuanwei and Fuyuan, China