Peramalan Jumlah Pengunjung Perpustakaan Universitas Pattimura Ambon Menggunakan Metode Dekomposisi

Perpustakaan merupakan pusat informasi yang harus dikelola dengan baik agar dapat memberikan manfaat semaksimal mungkin. Penelitian ini bertujuan untuk mengetahui fluktuasi dan meramalkan banyaknya pengunjung Perpustakaan Universitas Pattimura (UNPATTI) Ambon menggunakan metode Dekomposisi. Data yang digunakan adalah data sekunder jumlah pengunjung perpustakaan UNPATTI sejak tahun 2011– 2014 yang diperoleh dari perpustakaan UNPATTI. Prosedur penelitian dimulai dengan menganalisis komponen-komponen dekomposisi yaitu komponen trend (), musiman (), siklis () dan komponen acak () kemudian mengalikan nilai dari komponen-komponen tersebut. Hasil prediksi menunjukkan bahwa jumlah pengunjung pada tahun 2015 bulan Januari sebesar 1337 pengunjung, Februari 2932 pengunjung, Maret 3640 pengunjung, April 3791 pengunjung, Mei 4333 pengunjung, Juni 2571 pengunjung, Juli 806 pengunjung, Agustus 920 pengunjung, September 968 pengunjung, Oktober 4187 pengunung, November 4495 pengunjung dan Desember 2960 pengunjung. Dapat dilihat bahwa jumlah pengunjung meningkat pada bulan Mei dan November

Selection in Coastal Synechococcus (Cyanobacteria) Populations Evaluated from Environmental Metagenomes

Environmental metagenomics provides snippets of genomic sequences from all organisms in an environmental sample and are an unprecedented resource of information for investigating microbial population genetics. Current analytical methods, however, are poorly equipped to handle metagenomic data, particularly of short, unlinked sequences. A custom analytical pipeline was developed to calculate dN/dS ratios, a common metric to evaluate the role of selection in the evolution of a gene, from environmental metagenomes sequenced using 454 technology of flow-sorted populations of marine Synechococcus, the dominant cyanobacteria in coastal environments. The large majority of genes (98%) have evolved under purifying selection (dN/dS<1). The metagenome sequence coverage of the reference genomes was not uniform and genes that were highly represented in the environment (i.e. high read coverage) tended to be more evolutionarily conserved. Of the genes that may have evolved under positive selection (dN/dS>1), 77 out of 83 (93%) were hypothetical. Notable among annotated genes, ribosomal protein L35 appears to be under positive selection in one Synechococcus population. Other annotated genes, in particular a possible porin, a large-conductance mechanosensitive channel, an ATP binding component of an ABC transporter, and a homologue of a pilus retraction protein had regions of the gene with elevated dN/dS. With the increasing use of next-generation sequencing in metagenomic investigations of microbial diversity and ecology, analytical methods need to accommodate the peculiarities of these data streams. By developing a means to analyze population diversity data from these environmental metagenomes, we have provided the first insight into the role of selection in the evolution of Synechococcus, a globally significant primary producer