167 research outputs found
Shape coexistence in the odd-odd neutron-rich nucleus 98Y studied in the interacting boson model
Data on levels in 98Y have been investigated and interpreted using recently published information on band structure in this region. Calculation in the IBM framework suggests that the 1182 keV isomer is spherical 10- state with π g9/2 ν h11/2 configuration. This assignment implies that the 8.0 microsecond bandhead at 496 keV is a 4- state with highly probable π(422)5/2 ν(541)3/2 configuration. The level structure of 98Y is well reproduced by coupling the levels of its both spherical and deformed odd-A neighbors and the origin of the isomeric states is understood
Protein Folding Modulation in Cells Subject to Differentiation and Stress
Cytomimetic media are used to mimic the physicochemical properties of the cellular milieu in an in vitro experiment. The motivation is that compared to entire cells, they can be used efficiently in combination with a broad range of experimental techniques. However, the development and use of cytomimetic media is hampered by the lack of in-cell data that could be used as a hallmark to directly evaluate and improve the performance of cytomimetic media in different applications. Such data must include the study of specific biomolecular reactions in different cell types, different compartments of a single cells and different cellular conditions. In previous studies, model systems such as cancer cell lines, bacteria or oocytes were used. Here we studied how the environment of cells that undergo neuronal differentiation or proteostasis stress modulates the protein folding equilibrium. We found that NGF induced differentiation leads to a decrease of the melting temperature and a change of the folding mechanism. Proteomic changes that occur upon differentiation could explain this effect, however, we found that the crowding effect remained unchanged. Using MG132, a common proteasome inhibitor and inducer of the unfolded protein response, we show that changes to the quality control machinery modulate the folding equilibrium, leading to protein destabilization at prolonged stress exposure. Our study explores the range of protein folding modulation within cells subject to differentiation or stress that must be encountered in the development of cytomimetic media
The reaction cross section
The one- and two-step mechanisms of the reaction in
the range of incident proton kinetic energy 1.13-3.0 GeV have been
investigated. A remarkable peculiarity of the two-step mechanism which
incorporates subprocesses and is the so
called velocity matching providing the presence of all intermediate particles
nearly to the on-mass-shell. The differential cross section has been calculated
using a realistic model for the hypertritium wave function. The
maximum value of the cross section is estimated as 1nb/sr. The
contribution of the one-step mechanism with the elementary process into the cross section has been found to be two - three orders of
magnitude smaller in comparison with the two-step mechanism.Comment: 10 pages, Latex, 3 Postscript figure
Evidence of kaon nuclear and Coulomb potential effects on soft K+ production from nuclei
The ratio of forward K+ production on copper, silver and gold targets to that
on carbon has been measured at proton beam energies between 1.5 and 2.3 GeV as
a function of the kaon momentum p_K using the ANKE spectrometer at
COSY-Juelich. The strong suppression in the ratios observed for p_K<200-250
MeV/c can be ascribed to a combination of Coulomb and nuclear repulsion in the
K+A system. This opens a new way to investigate the interaction of K+-mesons in
the nuclear medium. Our data are consistent with a K+A nuclear potential of
V_K~20 MeV at low kaon momenta and normal nuclear density. Given the
sensitivity of the data to the kaon potential, the current experimental
precision might allow one to determine V_K to better than 3 MeV.Comment: 9 pages, 3 figures; changed conten
Protein Folding Modulation in Cells Subject to Differentiation and Stress
Cytomimetic media are used to mimic the physicochemical properties of the cellular milieu in an in vitro experiment. The motivation is that compared to entire cells, they can be used efficiently in combination with a broad range of experimental techniques. However, the development and use of cytomimetic media is hampered by the lack of in-cell data that could be used as a hallmark to directly evaluate and improve the performance of cytomimetic media in different applications. Such data must include the study of specific biomolecular reactions in different cell types, different compartments of a single cells and different cellular conditions. In previous studies, model systems such as cancer cell lines, bacteria or oocytes were used. Here we studied how the environment of cells that undergo neuronal differentiation or proteostasis stress modulates the protein folding equilibrium. We found that NGF induced differentiation leads to a decrease of the melting temperature and a change of the folding mechanism. Proteomic changes that occur upon differentiation could explain this effect, however, we found that the crowding effect remained unchanged. Using MG132, a common proteasome inhibitor and inducer of the unfolded protein response, we show that changes to the quality control machinery modulate the folding equilibrium, leading to protein destabilization at prolonged stress exposure. Our study explores the range of protein folding modulation within cells subject to differentiation or stress that must be encountered in the development of cytomimetic media
Phenomenological analysis of K+ meson production in proton-nucleus collisions
Total and differential cross sections from literature, on the production of
K+ mesons in pA interactions at projectile energies between T=0.8 and 2.9 GeV,
covering the transition across the free nucleon-nucleon threshold at 1.58 GeV,
have been investigated. From the target-mass dependence of the production cross
sections no evidence for the expected change of the dominant reaction mechanism
from two-step to direct kaon production was found. At T=1.0 GeV the A
dependences of the total cross sections and of the most recent data from
COSY-Juelich, differential cross sections measured under forward angles, are
strongly different. The invariant K+ production cross sections show an overall
exponential scaling behavior with the squared four-momentum transfer between
the beam proton and the produced K+ meson for t< -0.05 GeV^2 independent of the
beam energy and emission angle. The data from COSY-Juelich reveal a strongly
different t dependence in the region of t>0 GeV^2. Further data at forward
angles and different beam energies should be taken in order to explore this
region of kinematically extreme conditions.Comment: 9 Pages, 11 Figure
Fission-Residues Produced in the Spallation Reaction 238U+p at 1 A GeV
Fission fragments from 1 A GeV 238U projectiles irradiating a hydrogen target
were investigated by using the fragment separator FRS for magnetic selection of
reaction products including ray-tracing and DE-ToF techniques. The momentum
spectra of 733 identified fragments were analysed to provide isotopic
production cross sections, fission-fragment velocities and recoil momenta of
the fissioning parent nuclei. Besides their general relevance, these quantities
are also demanded for applications. Calculations and simulations with codes
commonly used and recently developed or improved are compared to the data.Comment: 60 pages, 21 figures, 4 tables, 2 appendices (15 pages
Forward K+ production in subthreshold pA collisions at 1.0 GeV
K+ meson production in pA (A = C, Cu, Au) collisions has been studied using
the ANKE spectrometer at an internal target position of the COSY-Juelich
accelerator. The complete momentum spectrum of kaons emitted at forward angles,
theta < 12 degrees, has been measured for a beam energy of T(p)=1.0 GeV, far
below the free NN threshold of 1.58 GeV. The spectrum does not follow a thermal
distribution at low kaon momenta and the larger momenta reflect a high degree
of collectivity in the target nucleus.Comment: 4 pages, 3 figure
Non-canonical Hedgehog signaling mediates profibrotic hematopoiesis-stroma crosstalk in myeloproliferative neoplasms.
The role of hematopoietic Hedgehog signaling in myeloproliferative neoplasms (MPNs) remains incompletely understood despite data suggesting that Hedgehog (Hh) pathway inhibitors have therapeutic activity in patients. We aim to systematically interrogate the role of canonical vs. non-canonical Hh signaling in MPNs. We show that Gli1 protein levels in patient peripheral blood mononuclear cells (PBMCs) mark fibrotic progression and that, in murine MPN models, absence of hematopoietic Gli1, but not Gli2 or Smo, significantly reduces MPN phenotype and fibrosis, indicating that GLI1 in the MPN clone can be activated in a non-canonical fashion. Additionally, we establish that hematopoietic Gli1 has a significant effect on stromal cells, mediated through a druggable MIF-CD74 axis. These data highlight the complex interplay between alterations in the MPN clone and activation of stromal cells and indicate that Gli1 represents a promising therapeutic target in MPNs, particularly that Hh signaling is dispensable for normal hematopoiesis
Non-canonical Hedgehog signaling mediates profibrotic hematopoiesis-stroma crosstalk in myeloproliferative neoplasms
The role of hematopoietic Hedgehog signaling in myeloproliferative neoplasms (MPNs) remains incompletely understood despite data suggesting that Hedgehog (Hh) pathway inhibitors have therapeutic activity in patients. We aim to systematically interrogate the role of canonical vs. non-canonical Hh signaling in MPNs. We show that Gli1 protein levels in patient peripheral blood mononuclear cells (PBMCs) mark fibrotic progression and that, in murine MPN models, absence of hematopoietic Gli1, but not Gli2 or Smo, significantly reduces MPN phenotype and fibrosis, indicating that GLI1 in the MPN clone can be activated in a non-canonical fashion. Additionally, we establish that hematopoietic Gli1 has a significant effect on stromal cells, mediated through a druggable MIF-CD74 axis. These data highlight the complex interplay between alterations in the MPN clone and activation of stromal cells and indicate that Gli1 represents a promising therapeutic target in MPNs, particularly that Hh signaling is dispensable for normal hematopoiesis.</p
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