Značaj galektina-1 kao prognostičkog faktora u lečenju klasičnog Hočkinovog limfoma

In classical Hodgkin’s lymphoma (cHL) the existing prognostic scoring systems do not include markers that adequately reflect the interaction of malignant Hodgkin and Reed-Sternberg (HRS) cells and tumor environment. The research objective is to determine the relationship between serum Galectin-1 (Gal-1) and soluble CD163 (sCD163) and the clinical status of patients with cHL, with special emphasis on the presence of relapse, progression, or resistance to the therapy applied. The research included 79 patients. Of this number, 63 were patients with cHL, and the control group consisted of 16 healthy volunteers. The study group of 63 patients with cHL included a subgroup of newly diagnosed patients without therapy, newly diagnosed patients with therapy, patients with relapse and progression of the disease, and primary refractory patients during 2014 and 2015. Analysis of the levels of sCD163 and sGal-1 within a group of patients suffering from cHL has shown that the values of both molecules were higher in relapsed patients and the subgroup with progressive disease comparing the subgroup of newly diagnosed patients without therapy or patients with therapy onset. Determination of Gal-1 and sCD163 levels is simple and reliable analysis that can contribute to the identification of high-risk patients with cHL, and deserves inclusion in current prognostic scoring systems

Globalizacija, digitalizacija i porodični biznis: 4.0 ERA

"Možeš odbiti da se promeniš, ali svet će se svejedno promeniti” Isak Adižes Istoriju obeležavaju različiti događaji koji predstavljaju promenu u mnogim socijalnim, kulturnim, ekonomskim i tehnološkim sferama. Industrijska revolucija jedna je od najpoznatijih. Sve te promene širom sveta dovele su do dramatičnih promena u načinu poslovanja i globalne povezanosti. Nesporna je činjenica da se svet i privreda u njemu zadnjih decenija izuzetno i ubrzano menjaju. Četvrta industrijska revolucija bazira se na tehnološkom i drugim pratećim znanjima, kao i na modelima privređivanja, koji su sve manje manuelnog, a sve više umnog i digitalnog karaktera. U ‘’Novom dobu’’ koje se ubrzano stvara, čak i velika preduzeća, koja proizvode ogromne količine robe, zapošljavaju sve manje radnika. Umesto ljudi rade kompjuteri, roboti i druge tzv. “pametne mašine”. Reč je o kreiranju sve složenijih procesnih postrojenja i sve “pametnijih” mašina ili mašinskih postupaka. Život i rad se sve više odvijaju preko aplikacija. Pod uticajem novih tehnologija došlo je do velike razmene podataka koja je generisala više vrednosti nego međunarodna trgovina. Novi trendovi industrijske revolucije 4.0 pomažu i razvijaju bolje poslovanje u malim i srednjm preduzećima. Ti novi trendovi su: novi biznis modeli; povećanje produktivnosti; veći kvalitet proizvoda; veći prihodi. Kroz ovaj rad smo pokušali da ukažemo na neke izazove za porodične kompanije u četvrtoj industrijskoj revoluciji 4.0. Da bi napredovale u Četvrtoj industrijskoj revoluciji, kompanije moraju da obezbede da njihovi radnici budu adekvatno opremljeni kroz usavršavanje i prekvalifikaciju, a zatim da zaposle nove ljude kada je to potrebno. Nova revolucija donosi nove dimenzije načina na koji mislimo, ponašamo se i organizujemo život. Promene će biti sve brže i obimnije, a ljudi sve zavisniji od tehnologije. Svedoci smo sveopšte digitalizacije društva u kome živimo. Što je zemlja razvijenija, to digitalizacija brže stiže u živote stanovnika. Brže od same digitalizacije dolaze najave kako će se digitalizacija sprovoditi. Digitalizaciju ne možemo izbeći, ali moramo biti svesni pretnji koje ona donosi

Optimal airfoil design and wing analysis for solar-powered high altitude platform station

The ability of flying continuously over prolonged periods of time has become target of numerous research studies performed in recent years in both the fields of civil aviation and unmanned drones. High altitude platform stations are aircrafts that can operate for an extended period of time at altitudes 17 km above sea level and higher. The aim of this paper is to design and optimize a wing for such platforms and computationally investigate its aerodynamic performance. For that purpose, two-objective genetic algorithm, class shape transformation and panel method were combined and used to define different airfoils with the highest lift-to-drag ratio and maximal lift coefficient. Once the most suitable airfoil was chosen, polyhedral half-wing was modeled and its aerodynamic performances were estimated using the CFD approach. Flow simulations of transitional flow at various angles-of-attack were realized in ANSYS FLUENT and various quantitative and qualitative results are presented, such as aerodynamic coefficient curves and flow visualizations. In the end, daily mission of the aircraft is simulated and its energy requirement is estimated. In order to be able to cruise above Serbia in July, an aircraft weighing 150 kg must accumulate 17 kWh of solar energy per day

Lateral-Directional Aerodynamic Optimization of a Tandem Wing UAV Using CFD Analyses

This paper presents the second stage of a tandem fixed-wing unmanned aerial vehicle (UAV) aerodynamic development. In the initial stage, the UAV was optimized by analyzing its characteristics only in symmetrical flight conditions. Posted requirements were that both wings should produce relevant positive lift, the initial stall must occur on the front wing first, the center of pressure should be close to the center of gravity, and longitudinal static stability should be in the optimum range. Computational fluid dynamic (CFD) analyses were performed, where the applied calculation model was derived from the authors’ previous successful projects. The eighth version TW V8 has satisfied all longitudinal requirements. Lateral-directional CFD analyses of V8 showed that the ratio of the lateral and directional stability at the nominal cruising regime was optimal, but both lateral and directional static stabilities were too high. On further development versions, the lower vertical tail was eliminated, a negative dihedral was implemented on the front wing, and four inverted blended winglets were added. Version TW V14 has largely improved lateral and directional stability characteristics, while their optimum ratio at the cruising regime was preserved. Longitudinal characteristics were also well preserved. Maximum lift coefficient and lift-to-drag ratio were increased, compared to the V8

A hybrid rans-les method with compressible k-omegaSSTSAS turbulence model for high Reynolds number flow applications

Trodimenzionalno, kompresibilno, viskozno i nestacionarno transonično turbulentno strujanje oko krila modelirano je hibridnom RANS-LES metodom, kombiniranom s kompresibilnim k-omegaSSTSAS turbulentnim modelom. Rabljeni pristup bazira se na podjeli utjecaja fluktuirajućih i osrednjenih polja brzina u okviru subtenzora i modeliranju svakog od njih odgovarajućim turbulentnim viskozitetom. U proračunu, "RANS mod" se koristi u područjima strujnog polja koja se s prihvatljivom točnošću mogu tretirati kao stacionarna, npr. u graničnom sloju, dok se "LES mod" primjenjuje u dominantno nestacionarnim područjima, daleko od krila. Diskretizacija jednadžbi strujanja vrši se metodom konačnih razlika na nestrukturiranoj mreži. Metoda je verificirana na Onera M6 krilu. Paralelizacija se provodi dekomponiranjem mreže na subdomene i uporabom Open MPI tehnologije. Implementacija turbulentnog modela obavljena je uporabom OpenFOAM-a. Simulacija strujanja provedena je i u ANSYS Fluent-u, a rezultati dobiveni pomoću ove dvije metode uspoređeni su kako međusobno, tako i s Onera M6 eksperimentom.Three-dimensional, compressible, viscous and transient transonic turbulent flow over the wing was simulated by a hybrid RANS-LES modelling method, combined with the compressible k-omegaSSTSAS turbulence model. This approach is based on dividing the contribution of the fluctuating and the averaged velocity fields in the subgrid tensor, and modelling each of them with its corresponding turbulent viscosity. The "RANS mode" is used in flow field domains which can be treated with acceptable accuracy as relatively steady, such as in the boundary layer, while the "LES mode" is applied in the dominantly unsteady regions, far from the wing. Discretization of the governing equations is performed by Finite Volume Method on unstructured mesh. The method has been verified on the Onera M6 wing. The parallelization is achieved by decomposing the mesh into sub-domains and using the Open MPI technology. The implementation of turbulence model has been done using OpenFOAM. The flow simulation was also performed using ANSYS Fluent, and the results of the two methods were compared mutually, and with the Onera M6 experiment

IMMOBILIZATION OF TYRAMINE-HRP ONTO TYRAMIDECARBOXYMETHYL CELLULOSE MATRIX FOR WASTEWATER TREATMENT

Horseradish peroxidase (HRP, E.C. 1.11.1.7) catalyzes oxidation of aqueous aromatic compounds using hydrogen peroxide. Enzymatic treatment methods for phenol removal from wastewaters has become an efficient and environmentally friendly alternative for the traditional methods. Carboxymethyl cellulose (CMC) derivative with tyramine attached via amide bond to carboxyl groups has been chosen as carrier for immobilization. In effort to overcome the main disadvantage of entrapment immobilization method, loss of enzyme activity due to washing out from the carrier, HRP was modified in a reductive amination reaction and tyramine was bound to the enzyme. Immobilization of tyramine-HRP onto tyramide-carboxymethyl cellulose carrier was carried in an emulsion polymerization reaction that produced carboxymethyl cellulose microbeads. The highest specific activity of the obtained biocatalyst was 0.227 U/ml and after 48h of storage 0.197 U/ml. Immobilized tyramine-HRP retained 87% of activity after 48 h. Immobilized HRP is a suitable candidate for wastewater treatment

Wastewater treatment by aminated peroxidase in alginate hydrogel

Phenols are highly toxic organic compounds found in wastewater due to various industries’ pollution. Its removal is of great importance for human and animal health. Enzymatic wastewater treatment has several advantages over traditional methods. Enzyme immobilization onto solid carriers enables its reusability and lowers the cost of treatment. In this work, immobilized horseradish peroxidase on chemically modified alginate hydrogel was tested for phenol removal. The reusability of the tested immobilizate was monitored in repeated cycles. After five consecutive cycles, the remaining activity of the immobilized enzyme was 54%. The obtained result shows the potential for using this immobilizate for wastewater treatment

Association of GSTT1 and GSTM1 gene polymorphisms with susceptibility to autoimmune diseases: A preliminary study

Autoimmune diseases (ADs) are chronic conditions initiated by the loss of immunological tolerance to self-antigens. The aim of this study was to detrmine how polymorphisms of glutathione S-transferases T1 and M1 (GSTT1 and GSTM1) genes influences on the occurrence of two autoimmune diseases: multiple sclerosis (MS) and Hashimoto's thyroditis (HT). A multiplex polymerase chain reaction (PCR) was used to detect the deletions in GSTT1 and GSTM1 genes. Our results showed that patients with ADs had significantly higher (p 0.05). This study suggests the potential role of GSTM1 deletion on ADs susceptibility, but on the other hand this study should be repeated in other patients with the same or similar diagnosis of ADs

The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats

Introduction: Diabetic retinopathy (DR) is not cured efficiently and changes of lifestyle measures may alleviate its course. The aim of our study was to investigate the effects of shortened daily photoperiod on inflammation and visual cycle in rat retina and retinal pigment epithelium (RPE) in DR. Methodology: Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with a single intraperitoneal injection of streptozotocin (100 mg/kg); 3. group exposed to light/dark cycle 6/18h for 3 months (6/18); 4. diabetic group exposed to light/dark cycle 6/18h (DM+6/18). Retinal blood vessel permeability was estimated immunohistochemically based on lectin staining, while the expression of genes involved in the visual cycle (SOX9, LRAT, RPE65, OTX2), and inflammation (IL-1, TNF-α) was determined by qRT-PCR in the retina and RPE. Results: Shortened photoperiod reduced neovascularisation and the expression of IL-1 and TNF-α in both retina and RPE. The expression of IL-1 and TNF-α genes in the retina was significantly higher in DM vs. control group (P=0.001). In contrast, retinal IL-1 and TNF-α expressions were significantly lower in DM+6/18 vs. DM group (P=0.001). The expression of IL-1 and TNF-α in RPE was significantly higher in DM vs. control group, however the expression of these genes was significantly lower in DM+6/18 vs. DM group (PIL-1=0.008 and PTNF-α=0.002). The expression of visual cycle genes was significantly up-regulated in RPE in DM+6/18 vs. DM group (P=0.001). Conclusion: Shortened daily photoperiod reduces blood vessel permeability in DR via its anti-inflammatory effect associated with accelerated visual cycle in the retina.Poster Session: Neuroimmunoendocrine Interaction

Značaj galektina-1 kao prognostičkog faktora u lečenju klasičnog Hočkinovog limfoma

In classical Hodgkin’s lymphoma (cHL) the existing prognostic scoring systems do not include markers that adequately reflect the interaction of malignant Hodgkin and Reed-Sternberg (HRS) cells and tumor environment. The research objective is to determine the relationship between serum Galectin-1 (Gal-1) and soluble CD163 (sCD163) and the clinical status of patients with cHL, with special emphasis on the presence of relapse, progression, or resistance to the therapy applied. The research included 79 patients. Of this number, 63 were patients with cHL, and the control group consisted of 16 healthy volunteers. The study group of 63 patients with cHL included a subgroup of newly diagnosed patients without therapy, newly diagnosed patients with therapy, patients with relapse and progression of the disease, and primary refractory patients during 2014 and 2015. Analysis of the levels of sCD163 and sGal-1 within a group of patients suffering from cHL has shown that the values of both molecules were higher in relapsed patients and the subgroup with progressive disease comparing the subgroup of newly diagnosed patients without therapy or patients with therapy onset. Determination of Gal-1 and sCD163 levels is simple and reliable analysis that can contribute to the identification of high-risk patients with cHL, and deserves inclusion in current prognostic scoring systems