585 research outputs found
Effects of exercise training on atrophy gene expression in skeletal muscle of mice with chronic allergic lung inflammation
We evaluated the effects of chronic allergic airway inflammation and of treadmill training (12 weeks) of low and moderate intensity on muscle fiber cross-sectional area and mRNA levels of atrogin-1 and MuRF1 in the mouse tibialis anterior muscle. Six 4-month-old male BALB/c mice (28.5 ± 0.8 g) per group were examined: 1) control, non-sensitized and non-trained (C); 2) ovalbumin sensitized (OA, 20 µg per mouse); 3) non-sensitized and trained at 50% maximum speed _ low intensity (PT50%); 4) non-sensitized and trained at 75% maximum speed _ moderate intensity (PT75%); 5) OA-sensitized and trained at 50% (OA+PT50%), 6) OA-sensitized and trained at 75% (OA+PT75%). There was no difference in muscle fiber cross-sectional area among groups and no difference in atrogin-1 and MuRF1 expression between C and OA groups. All exercised groups showed significantly decreased expression of atrogin-1 compared to C (1.01 ± 0.2-fold): PT50% = 0.71 ± 0.12-fold; OA+PT50% = 0.74 ± 0.03-fold; PT75% = 0.71 ± 0.09-fold; OA+PT75% = 0.74 ± 0.09-fold. Similarly significant results were obtained regarding MuRF1 gene expression compared to C (1.01 ± 0.23-fold): PT50% = 0.53 ± 0.20-fold; OA+PT50% = 0.55 ± 0.11-fold; PT75% = 0.35 ± 0.15-fold; OA+PT75% = 0.37 ± 0.08-fold. A short period of OA did not induce skeletal muscle atrophy in the mouse tibialis anterior muscle and aerobic training at low and moderate intensity negatively regulates the atrophy pathway in skeletal muscle of healthy mice or mice with allergic lung inflammation.FAPESPCNP
255 Screening glucose disorders in cystic fibrosis: Continuous subcutaneous glucose monitoring compared to oral glucose tolerance test
Effective degradation of phenacetin in wastewater by (photo)electro-Fenton processes: Investigation of variables, acute toxicity, and intermediates
Electro-Fenton (EF) and solar photoelectro-Fenton (SPEF) processes were employed at different scales to degrade phenacetin (PNT), the first synthetic analgesic. EF experiments were conducted at lab scale, whereas SPEF experiments were performed in an 8 L pre-pilot plant using an electrochemical filter-press cell. Under optimal conditions (25 mg L−1 PNT, 25.3 mg L−1 Fe2+, and current density of 59.5 mA cm−2), EF resulted in degradation and mineralization degrees of 83.9% and 45.2% at 14 and 230 min, respectively. Similarly, PNT was spiked into real wastewater from a municipal secondary treatment plant, resulting in degradation of 68.0% and mineralization of 39.4%, with an energy consumption of 7.0 kWh g−1. The optimal conditions of SPEF (16.8 mg L−1 Fe2+ and current density of 45.9 mA cm−2) led to degradation and mineralization degrees of 55.9% and 37.1% at 36 and 181 min, respectively, with a low energy consumption of 0.142 kWh g−1. Both processes effectively detoxified the solutions, as demonstrated by tests with Artemia salina and Lactuca sativa. Three distinct degradation pathways were proposed based on the identification of eleven reaction intermediates formed upon •OH attack. In conclusion, the low energy cost of the SPEF process underscores its potential for pharmaceutical degradation in wastewater
Molecular detection of Rickettsia rickettsii, Ehrlichia canis and Rangelia vitalli in Rhipicephalus sanguineus senso latu collected from dogs in Brazil
ABSTRACT This study evaluated by molecular methods the presence of major canine tick-borne agents in ticks infesting domestic dogs of a hospital population in a neglected area of the southern zone of the São Paulo Metropolitan region, which is characterized by an extensive urban area surrounded and interspersed by forest remnants of the original Atlantic rainforest. During 2017, 106 tick specimens - 71 adults and 33 nymphs of Rhipicephalus sanguineus sensu lato (s.l.), and two adults of Amblyomma aureolatum - were collected from 41 dogs that were attended in a Veterinary Teaching Hospital in São Paulo City, Brazil. By molecular analyses, 4.2% (3/71) of the R. sanguineus s.l. adult ticks contained the bacterium Rickettsia rickettsii, 2.8% (2/71) contained the bacterium Ehrlichia canis, and 4.2% (3/71) contained the protozoan Rangelia vitalii. These results indicate that domestic dogs of the southern zone of the São Paulo metropolitan region might be exposed to three of the major tick-borne agents affecting dogs in Brazil, R. rickettsii, E. canis and R. vitalii. In addition, the findings reinforce the circulation of the human pathogen R. rickettsii in the study area in a likely enzootic cycle involving dogs and R. sanguineus ticks
Deciphering The Preservation Of Fossil Insects: A Case Study From The Crato Member, Early Cretaceous Of Brazil
Exceptionally well-preserved three-dimensional insects with fine details and even labile tissues are ubiquitous in the Crato Member Konservat Lagerstätte (northeastern Brazil). Here we investigate the preservational pathways which yielded such specimens. We employed high resolution techniques (EDXRF, SR-SXS, SEM, EDS, micro Raman, and PIXE) to understand their fossilisation on mineralogical and geochemical grounds. Pseudomorphs of framboidal pyrite, the dominant fossil microfabric, display size variation when comparing cuticle with inner areas or soft tissues, which we interpret as the result of the balance between ion diffusion rates and nucleation rates of pyrite through the originally decaying carcasses. Furthermore, the mineral fabrics are associated with structures that can be the remains of extracellular polymeric substances (EPS). Geochemical data also point to a concentration of Fe, Zn, and Cu in the fossils in comparison to the embedding rock. Therefore, we consider that biofilms of sulphate reducing bacteria (SRB) had a central role in insect decay and mineralisation. Therefore, we shed light on exceptional preservation of fossils by pyritisation in a Cretaceous limestone lacustrine palaeoenvironment. © 2016 Osés et al.20161
LÍQUIDO FOLICULAR EQÜINO NA MATURAÇÃO IN VITRO DE OÓCITOS BOVINOS
O desenvolvimento embrionário de oócitos bovinos maturados in vitro (MIV) foi
avaliado em meio suplementado com líquido folicular eqüino (Lfe). Foram distribuídos 1045 oócitos
em 11 repetições formando três grupos tratamentos (T1, T2, T3) e um controle (C). O meio de
maturação utilizado foi o TCM-199 acrescido de piruvato de sódio, hormônio folículo estimulante
recombinante (rFSHh) e hormônio luteinizante equino (LHe). Suplementou-se esse meio com
10% de soro de égua em estro para o grupo controle e para T1, T2 e T3, o meio foi suplementado
com 5, 10, e 20% de LFe, respectivamente. Os oócitos foram maturados in vitro (MIV) por 24h.
A fecundação in vitro (FIV) foi realizada em meio Talp-Fert. A MIV e a FIV foram realizadas em
estufa a 39ºC com 5% de CO2 em ar e umidade saturada. Os zigotos foram cultivados em meio
SOFaaci, sob óleo mineral no interior de bolsas plásticas gaseificadas. As taxas de clivagem e
de blastocistos foram observadas diariamente (D), e em D7, foram superiores (P0,05) às do grupo controle. Em D9, a taxa
de blastocistos do T2 foi superior (P0,05). O LFe, na concentração de 10%
pode ser utilizado, em substituição ao soro de égua em estro para suplementar o meio de MIV
de oócitos bovinos.
Equine follicular fluid on in vitro maturation of bovine oocytes
Abstract
Embryo development of bovine oocytes was evaluated using maturation medium
supplemented with equine follicular fluid (eFF). One thousand and forty five (1045) oocytes were distributed
in 11 replications forming three treatment groups (T1, T2 e T3) and one Control (C). TCM-199 added with
sodium pyruvate, rFSHh and LHe was used as maturation medium. This medium was supplemented with
10% estrous mare serum for Control group, and 5, 10, and 20% eFF, respectively, for T1, T2 e T3
groups. In vitro maturation (IVM) of all groups was performed during 24h. In vitro fertilization (IVF) was
performed in TALP-FERT medium. IVM and IVF were carried out in an incubator at 39ºC with 5% CO2 in
air and saturated humidity. Zygotes were cultured in SOFaaci medium, under mineral oil in gasified
bags. Cleavage and blastocyst rates were daily observed (D), and at D7, were higher (P0.05) for those from control group. At D9, blastocyst rate of T2
was higher (P0.05). The eFF, at a 10% concentration, can replace the use of
estrous mare serum to supplement the IVM medium of bovine oocytes
How does the replacement of rice flour with flours of higher nutritional quality impact the texture and sensory profile and acceptance of gluten-free chocolate cakes?
Gluten-free bakery products usually use rice flour as substitute for wheat flour. This paper aims to evaluate whether and how the substitution of rice flour for sorghum and teff flour changes the overall acceptance, texture and sensory profile of gluten-free chocolate cakes. An experimental design composed of three factors (rice, sorghum and teff flours) was developed, and formulations were analysed by acceptance test and fibre content. Four formulations were submitted to sensory descriptive analysis. The formulations did not show significant differences in the overall acceptance although the sensory profile has changed. The texture was affected by the type of flour, being the optimised formulation the softer among the samples. From these data, it can be concluded that it is possible to replace rice flour with sorghum and teff flour in chocolate cake formulations, since the change in the sensory profile did not affect the acceptance of the products.info:eu-repo/semantics/publishedVersio
Primeiro registro de Amblyomma geayi (Acari: ixodidae) em preguiça (Bradypus variegatus) no estado do Acre, Amazônia Ocidental: relato de caso
Blood parameters and measurements of weight in the rehabilitation of Magellanic penguins (Spheniscus magellanicus, Foster 1781)
- …