Prevalência de vaginose bacteriana e fatores associados em mulheres que fazem sexo com mulheres

Objetivo: descrever a prevalência de vaginose bacteriana e fatores associados em mulheres que fazem sexo com mulheres. Método: trata-se de estudo transversal, descritivo e analítico com 150 mulheres. O padrão de microbiota vaginal foi analisado por microscopia do conteúdo vaginal corado pelo método de Gram. Amostras de secreção endocervical foram coletadas com cytobrush para a pesquisa de endocervicites por Chlamydia trachomatis e para infecção por Papilomavírus Humano por meio de reação em cadeia da polimerase. Dados sociodemográficos, de comportamento sexual e de história clínica foram obtidos por entrevista. Regressão logística foi realizada para identificar fatores de risco independentemente associados à vaginose bacteriana. Resultados: dentre as 150 participantes, 71 (47,3%) tinham alguma alteração da microbiota vaginal, 54 (36,0%) vaginose bacteriana e 12 (8,0%) Flora II. A variável independentemente associada com vaginose bacteriana foi o uso de acessórios sexuais [2,37(1,13-4,97), p=0,022]. Conclusão: a elevada prevalência de vaginose bacteriana entre mulheres que fazem sexo com mulheres aponta a necessidade de rastreio nessa população. O uso de acessórios sexuais associado a esse agravo sugere a possibilidade de transmissão de fluidos sexuais entre as parceiras durante o ato sexual, o que demonstra necessidade de ações de educação em saúde sexual e reprodutiva.Objective: to describe the prevalence of bacterial vaginosis and factors associated among women who have sex with women. Method: cross-sectional, descriptive and analytical study with 150 women. The vaginal microbiota profile was analyzed by microscopic examination of vaginal swabs according to the Gram method. Endocervical samples were collected with cytobrush for the investigation of endocervicitis by Chlamydia trachomatis. The polymerase chain reaction was used to diagnosis Human Papillomavirus infection. Socio-demographic data, sexual behavior and clinical history were obtained through an interview. Logistic regression was performed to identify risk factors independently associated with bacterial vaginosis. Results: among the 150 participants, 71 (47.3%) presented some alteration in the vaginal microbiota, 54 (36.0%) bacterial vaginosis and 12 (8.0%) Flora II. The variable independently associated with bacterial vaginosis was the use of sexual accessories [2.37(1.13-4.97), p=0.022]. Conclusion: the high prevalence of bacterial vaginosis among women who have sex with women indicates the need for screening this population and association between use of sexual accessories and this disease suggests the possibility of transmission of sexual fluids between the partners during the sexual act, which demonstrates the need for educational actions on sexual and reproductive health.Objetivo: describir la prevalencia de la vaginosis bacteriana y los factores asociados en mujeres que tienen sexo con mujeres. Método: se trata de un estudio transversal, descriptivo y analítico realizado entre 150 mujeres. El patrón de microbiota vaginal se analizó por microscopía del contenido vaginal teñido por el método de Gram. Se recolectaron muestras de secreción endocervical con un citocepillo para investigar la endocervicitis por Chlamydia trachomatis y la infección por el Virus del Papiloma Humano mediante la reacción en cadena de la polimerasa. De la entrevista se obtuvieron datos sociodemográficos, de comportamiento sexual y del historial clínico. Se llevó a cabo una regresión logística para identificar factores de riesgo asociados independientemente a la vaginosis bacteriana. Resultados: entre las 150 participantes, el 71 (47,3%) tenía alteración de la microbiota vaginal, el 54 (36,0%), vaginosis bacteriana y el 12 (8,0%), Flora II. La variable asociada independientemente a la vaginosis bacteriana se debió al uso de accesorios sexuales [2,37(1,13-4,97), p=0,022]. Conclusión: la prevalencia elevada de vaginosis bacteriana entre mujeres que tienen sexo con mujeres señala la necesidad de estudiar dicha población, y el uso de accesorios sexuales asociado a este agravante sugiere la posibilidad de transmisión de fluidos sexuales entre las compañeras durante el acto sexual, razón por la cual deben llevarse a cabo acciones de educación en salud sexual y reproductiva

Santé mentale de l´étudiant psychologue

O artigo apresenta o resultado da pesquisa “Saúde mental do estudante de Psicologia: possíveis implicações para sua atuação profissional”, que foi apoiada pelo Programa Pesquisa Produtividade, da Universidade Estácio de Sá. O objetivo foi verificar se as vivências acadêmicas do estudante de Psicologia da Universidade Estácio de Sá, campus Nova Iguaçu, que está em contato com o sofrimento psíquico e conteúdos acadêmicos relacionados a subjetividade humana, afetam sua saúde mental gerando transtornos mentais menores; e como isso pode, potencialmente, trazer implicações para a prática profissional do psicólogo. Trinta e sete estudantes do curso de Psicologia (1º e 10º períodos), foram submetidos à Entrevista Clínica Estruturada para os Transtornos do DSM-5 (SCID-5) para verificação do objetivo. A conclusão indica que os estudantes de primeiro período referem como maior dificuldade associada à graduação, o manejo de tempo para as demandas do curso e outras demandas pessoais, como trabalho e vida social. Já nos estudantes de décimo período são recorrentes as falas de cansaço e a preocupação com o mercado de trabalho. Os resultados sugerem que há um impacto da rotina da faculdade na vida dos estudantes.El artículo presenta el resultado de la investigación “Salud mental de los estudiantes de psicología: posibles implicaciones para su desempeño profesional”, que fue apoyada por el Programa de Investigación de Productividad, de la Universidad Estácio de Sá. El objetivo fue verificar si las experiencias académicas del estudiante de Psicología en la Universidad Estácio de Sá, en el campus de Nova Iguaçu, que está en contacto con la angustia psicológica y los contenidos académicos relacionados con la subjetividad humana, afectan su salud mental generando trastornos mentales menores; y cómo esto puede potencialmente tener implicaciones para la práctica profesional del psicólogo. Treinta y siete estudiantes de psicología (1º y 10º períodos) se sometieron a la entrevista clínica estructurada para trastornos del DSM-5 (SCID-5) para verificar el objetivo. La conclusión indica que los estudiantes de primer año informan la mayor dificultad asociada con la graduación, la administración del tiempo para las demandas del curso y otras demandas personales, como el trabajo y la vida social. En el décimo período, los estudiantes experimentan un cansancio y una preocupación recurrentes por el mercado laboral. Los resultados sugieren que hay un impacto de la rutina universitaria en la vida de los estudiantes.The article presents the result of the research “Psychology student mental health: possible implications for their professional performance”, which was supported by the Productivity Research Program, from Estácio de Sá University. The aim was to examine if the academic experiences of the Psychology student at Estácio de Sá University, Nova Iguaçu campus, which is in contact with psychological distress and academic contents related to human subjectivity, affect their mental health generating minor mental disorders; and how this can potentially have implications for the psychologist's professional practice. Thirty-seven Psychology students (1st and 10th periods) underwent the Structured Clinical Interview for DSM-5 Disorders (SCID-5) to verify the objective. The conclusion indicates that first-year students report the greatest difficulty associated with graduation, time management for the demands of the course and other personal demands, such as work and social life. In the tenth period students, there are recurrent tiredness and concern about the job market. The results suggest that there is an impact of college routine on students' lives.L'article présente le résultat de la recherche «Santé mentale des étudiants en psychologie: implications possibles pour leur performance professionnelle», soutenue par le programme de recherche sur la productivité de l'Université Estácio de Sá. L’objectif était de vérifier si les expériences académiques de l’étudiant en psychologie de l’Université Estácio de Sá, sur le campus de Nova Iguaçu, en contact avec la détresse psychologique et les contenus académiques liés à la subjectivité humaine avaient une incidence sur leur santé mentale, générant des troubles mentaux mineurs; et comment cela peut potentiellement avoir des implications pour la pratique professionnelle du psychologue. Trente-sept étudiants en psychologie (1ère et 10ème périodes) ont subi un entretien clinique structuré pour les troubles du DSM-5 (SCID-5) afin de vérifier l'objectif. La conclusion indique que les étudiants de première année signalent la plus grande difficulté associée à l’obtention du diplôme, à la gestion du temps imputable aux exigences du cours et à d’autres exigences personnelles, telles que le travail et la vie sociale. Dans la dixième période, les étudiants sont fatigués et préoccupés par le marché du travail. Les résultats suggèrent que la routine des collèges a un impact sur la vie des étudiants

Prevalencia de vaginosis bacteriana y factores asociados en mujeres que tienen sexo con mujeres

Objetivo: descrever a prevalência de vaginose bacteriana e fatores associados em mulheres que fazem sexo com mulheres. Método: trata-se de estudo transversal, descritivo e analítico com 150 mulheres. O padrão de microbiota vaginal foi analisado por microscopia do conteúdo vaginal corado pelo método de Gram. Amostras de secreção endocervical foram coletadas com cytobrush para a pesquisa de endocervicites por Chlamydia trachomatis e para infecção por Papilomavírus Humano por meio de reação em cadeia da polimerase. Dados sociodemográficos, de comportamento sexual e de história clínica foram obtidos por entrevista. Regressão logística foi realizada para identificar fatores de risco independentemente associados à vaginose bacteriana. Resultados: dentre as 150 participantes, 71 (47,3%) tinham alguma alteração da microbiota vaginal, 54 (36,0%) vaginose bacteriana e 12 (8,0%) Flora II. A variável independentemente associada com vaginose bacteriana foi o uso de acessórios sexuais [2,37(1,13-4,97), p=0,022]. Conclusão: a elevada prevalência de vaginose bacteriana entre mulheres que fazem sexo com mulheres aponta a necessidade de rastreio nessa população. O uso de acessórios sexuais associado a esse agravo sugere a possibilidade de transmissão de fluidos sexuais entre as parceiras durante o ato sexual, o que demonstra necessidade de ações de educação em saúde sexual e reprodutiva.Objective: to describe the prevalence of bacterial vaginosis and factors associated among women who have sex with women. Method: cross-sectional, descriptive and analytical study with 150 women. The vaginal microbiota profile was analyzed by microscopic examination of vaginal swabs according to the Gram method. Endocervical samples were collected with cytobrush for the investigation of endocervicitis by Chlamydia trachomatis. The polymerase chain reaction was used to diagnosis Human Papillomavirus infection. Socio-demographic data, sexual behavior and clinical history were obtained through an interview. Logistic regression was performed to identify risk factors independently associated with bacterial vaginosis. Results: among the 150 participants, 71 (47.3%) presented some alteration in the vaginal microbiota, 54 (36.0%) bacterial vaginosis and 12 (8.0%) Flora II. The variable independently associated with bacterial vaginosis was the use of sexual accessories [2.37(1.13-4.97), p=0.022]. Conclusion: the high prevalence of bacterial vaginosis among women who have sex with women indicates the need for screening this population and association between use of sexual accessories and this disease suggests the possibility of transmission of sexual fluids between the partners during the sexual act, which demonstrates the need for educational actions on sexual and reproductive health.Objetivo: describir la prevalencia de la vaginosis bacteriana y los factores asociados en mujeres que tienen sexo con mujeres. Método: se trata de un estudio transversal, descriptivo y analítico realizado entre 150 mujeres. El patrón de microbiota vaginal se analizó por microscopía del contenido vaginal teñido por el método de Gram. Se recolectaron muestras de secreción endocervical con un citocepillo para investigar la endocervicitis por Chlamydia trachomatis y la infección por el Virus del Papiloma Humano mediante la reacción en cadena de la polimerasa. De la entrevista se obtuvieron datos sociodemográficos, de comportamiento sexual y del historial clínico. Se llevó a cabo una regresión logística para identificar factores de riesgo asociados independientemente a la vaginosis bacteriana. Resultados: entre las 150 participantes, el 71 (47,3%) tenía alteración de la microbiota vaginal, el 54 (36,0%), vaginosis bacteriana y el 12 (8,0%), Flora II. La variable asociada independientemente a la vaginosis bacteriana se debió al uso de accesorios sexuales [2,37(1,13-4,97), p=0,022]. Conclusión: la prevalencia elevada de vaginosis bacteriana entre mujeres que tienen sexo con mujeres señala la necesidad de estudiar dicha población, y el uso de accesorios sexuales asociado a este agravante sugiere la posibilidad de transmisión de fluidos sexuales entre las compañeras durante el acto sexual, razón por la cual deben llevarse a cabo acciones de educación en salud sexual y reproductiva

BOVINE MEAT HAMBURGER WITH CHIA MIXED FLOUR, OATS AND LINSEED

The work aimed at developing formulations of hamburger using the chia mixed flour, oats and linseed, as a partial replacement to textured soy protein (PTS), commonly used in this product. Income analyzes, instrumental color and sensory analysis, were carried out indicating that the addition of 3% of the mixed flour reduced the loss of water after baking, indicating the probable contribution of the fibers present in the seed used for the preparation of flour for moisture retention in the product. Regarding color, significant differences (p<0.05) between the burgers were identified only in instrumental analysis, not being noticed by the sensory panelists. For sensory analysis, only the taste and texture attributes were significantly different, with higher averages for the formulation added to mixed flour, which contributed to the high levels of acceptability, above 70%. It highlights the possibility of product preparation adding new ingredients, examples of the chia mixed flour, oats and linseed, however there is a need for further analysis to characterize the hamburger

Differential expression of RAD51AP1 in ovarian cancer : effects of siRNA in vitro

Background: DNA double strand breaks can affect genome integrity potentially leading to cancer. RAD51-associated protein 1 (RAD51AP1), an accessory protein to RAD51, is critical for homologous recombination, a key DNA damage response pathway. Emerging studies indicate a novel role for RAD51AP1 in carcinogenesis. Here we provide additional insight into the role of RAD51AP1 in ovarian cancer (OvCa). Methods: Gene expression and patient phenotype data were obtained from TCGA and GTEX project consortia for bioinformatics analysis. Immunohistochemistry of OvCa tissue microarray was undertaken. Functional analyses were performed in a SKOV3 OvCa cell line with down-regulation of RAD51AP1 using siRNA. Results: RAD51AP1 is overexpressed at gene level in primary and recurrent OvCa compared to controls. At protein level, RAD51AP1 was up-regulated in low grade serous tumors compared to high grade OvCa. There was higher expression of RAD51AP1 in OvCa metastatic to lymph nodes compared to primary cancer samples. Gene enrichment analyses identified 12 differentially expressed genes (DEGs) related to OvCa, eight of which are also common in tissue from patients with type 2 diabetes mellitus (T2DM). Conclusions: RAD51AP1 is overexpressed in OvCa, Given the link between OvCa and T2DM, the eight-gene signature shows potential for predictive value

Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

Background: DNA double strand breaks can affect genome integrity potentially leading to cancer. RAD51-associated protein 1 (RAD51AP1), an accessory protein to RAD51, is critical for homologous recombination, a key DNA damage response pathway. Emerging studies indicate a novel role for RAD51AP1 in carcinogenesis. Here we provide additional insight into the role of RAD51AP1 in ovarian cancer (OvCa). Methods: Gene expression and patient phenotype data were obtained from TCGA and GTEX project consortia for bioinformatics analysis. Immunohistochemistry of OvCa tissue microarray was undertaken. Functional analyses were performed in a SKOV3 OvCa cell line with down-regulation of RAD51AP1 using siRNA. Results: RAD51AP1 is overexpressed at gene level in primary and recurrent OvCa compared to controls. At protein level, RAD51AP1 was up-regulated in low grade serous tumors compared to high grade OvCa. There was higher expression of RAD51AP1 in OvCa metastatic to lymph nodes compared to primary cancer samples. Gene enrichment analyses identified 12 differentially expressed genes (DEGs) related to OvCa, eight of which are also common in tissue from patients with type 2 diabetes mellitus (T2DM). Conclusions: RAD51AP1 is overexpressed in OvCa, Given the link between OvCa and T2DM, the eight-gene signature shows potential for predictive value

Incidence and risk factors for Preeclampsia in a cohort of healthy nulliparous pregnant women: a nested case-control study

The objective of this study is to determine the incidence, socio-demographic and clinical risk factors for preeclampsia and associated maternal and perinatal adverse outcomes. This is a nested case-control derived from the multicentre cohort study Preterm SAMBA, in five different centres in Brazil, with nulliparous healthy pregnant women. Clinical data were prospectively collected, and risk factors were assessed comparatively between PE cases and controls using risk ratio (RR) (95% CI) plus multivariate analysis. Complete data were available for 1,165 participants. The incidence of preeclampsia was 7.5%. Body mass index determined at the first medical visit and diastolic blood pressure over 75 mmHg at 20 weeks of gestation were independently associated with the occurrence of preeclampsia. Women with preeclampsia sustained a higher incidence of adverse maternal outcomes, including C-section (3.5 fold), preterm birth below 34 weeks of gestation (3.9 fold) and hospital stay longer than 5 days (5.8 fold) than controls. They also had worse perinatal outcomes, including lower birthweight (a mean 379 g lower), small for gestational age babies (RR 2.45 [1.52-3.95]), 5-minute Apgar score less than 7 (RR 2.11 [1.03-4.29]), NICU admission (RR 3.34 [1.61-6.9]) and Neonatal Near Miss (3.65 [1.78-7.49]). Weight gain rate per week, obesity and diastolic blood pressure equal to or higher than 75 mmHg at 20 weeks of gestation were shown to be associated with preeclampsia. Preeclampsia also led to a higher number of C-sections and prolonged hospital admission, in addition to worse neonatal outcomes9CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ401636/2013-5Bill and Melinda Gates FoundationGates Foundation [OPP1107597]; CNPqNational Council for Scientific and Technological Development (CNPq) [401636/2013-5

NOTIFICAÇÃO DA COVID-19

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