Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-7

F-β(E) for 48 h. B: Increased expression of Tn was detected by Western blot analysis in cell lysates after stimulation with TGF-β(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). D: Augmented expression of Tn protein was detected by flow cytometry in response to LTDcombined with TGF-β, compared with the effect of LTDalone. Difference from isotype control mean fluorescence intensity (MFI) is presented (mean ± SEM). Presence of 5 mM L-cysteine in the culture media did not affect the expression levels of Tn (D, E). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells. NS – non-significant.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-0

O LTD(A) and to LTE(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). C: Immunoperoxidase immunocytochemistry for Tn showing the basal expression of Tn and an increase by treatment with LTDand with LTE. Images are reproduced from a single experiment, but are representative findings of multiple staining experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-1

F-β(E) for 48 h. B: Increased expression of Tn was detected by Western blot analysis in cell lysates after stimulation with TGF-β(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). D: Augmented expression of Tn protein was detected by flow cytometry in response to LTDcombined with TGF-β, compared with the effect of LTDalone. Difference from isotype control mean fluorescence intensity (MFI) is presented (mean ± SEM). Presence of 5 mM L-cysteine in the culture media did not affect the expression levels of Tn (D, E). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells. NS – non-significant.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-5

BAY u9773. There was no statistical difference between the inhibitory effects of MLK and BAY u9773. Data are expressed as mean ± SEM (n = 12) *p < 0.05, **p < 0.01, and ***p < 0.001.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-2

not with LTE(B) for 48 h. RT-PCR analysis detected increased expression of Ln β2 chain mRNA in response to 100 nM LTD(D), but no effect with either LTE(D) or TGF-β(E). The increase in response to LTDwas concentration-dependent. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). *p < 0.05 and ***p < 0.001 vs. non-stimulated cells. C: Immunoperoxidase immunocytochemistry for Ln β2 chain showing no definite expression of Ln β2 chain by non-stimulated control cells with a slight increase after treatment with both LTDand LTE. Images are reproduced from a single experiment, but are representative findings of multiple staining experiments.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-6

O LTD(A) and to LTE(B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). C: Immunoperoxidase immunocytochemistry for Tn showing the basal expression of Tn and an increase by treatment with LTDand with LTE. Images are reproduced from a single experiment, but are representative findings of multiple staining experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-4

Xpression to the levels not differing from that in non-stimulated cells. Difference from isotype control mean fluorescence intensity (MFI) is presented. B: RT-PCR analysis of Tn mRNA showing inhibition of the cysteinyl leukotriene-induced up-regulation by both MLK and BAY u9773. Partial inhibition of Tn expression induced by combined LTDand TGF-βdown to the level not differing from that what was seen after stimulation with TGF-βalone was shown at both protein (A) and mRNA (B) level. There was no statistical difference between the inhibitory effects of MLK and BAY u9773 in any setting. Data are expressed as mean ± SEM (n = 12) *p < 0.05, **p < 0.01, and ***p < 0.001. NS – non-significant.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor-3

Rotein loaded. B: RT-PCR analysis of CysLTand CysLTmRNA. The graph represents densitometric results of CysLTand CysLT, normalized to β-actin (mean ± SEM) (n = 9). *p < 0.05. C: Indirect FITC-immunofluorescence staining showing localization of cysteinyl leukotriene receptors in non-stimulated cells. D: Representative flow cytometric analysis of CysLTand CysLTon BEAS-2B cells. The open histograms correspond to cells labeled with isotype control antibody and the shaded ones represent cells labeled with the respective anti-CysLT antibodies.<p><b>Copyright information:</b></p><p>Taken from "Synthesis of tenascin and laminin beta2 chain in human bronchial epithelial cells is enhanced by cysteinyl leukotrienes via CysLTreceptor"</p><p>http://respiratory-research.com/content/9/1/44</p><p>Respiratory Research 2008;9(1):44-44.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2412865.</p><p></p

Principal component analysis of the metabolic state of primary normal human bronchial epithelial cells cultivated in air-liquid interface after exposure to (A) e-cigarette liquid (ECL) (100 μM by nicotine) and (B) 10 μg/mL cigarette smoke condensate (CSC) (black solid lines).

<p>The time points at the arrow turns are 1, 2, 5, 7, and 13 h. Long dashes: Addition of 10 μM O-methyl-l-tyrosinyl-γ-l-glutamyl-l-cysteinylglycine to the cells having been exposed to ECL for 1 h (the arrow starts at 2 h time point), short dashes: Addition of 2 mM N-acetylcysteine to the cells having been exposed to ECL for 1 h (the arrow starts at 2 h time point). For each treatment and for any time point, n = 3 (gray circles). Error bars indicate standard errors of means. PComp1 and PComp2 are the two principal components describing the highest fraction of the effect of cells’ metabolome.</p

Time dynamics of the expression of the metabolites of primary normal human bronchial epithelial cells (HBEC) cultured in air-liquid interface being affected by e-cigarette liquid (ECL) (100 μM by nicotine) (black lines) and 10 μg/mL cigarette smoke condensate (CSC) (grey lines) for 13 h.

<p>Solid lines: HBEC treated with ECL (solid black lines) or with CSC (solid grey lines). Long black dashes: HBEC treated with ECL and 10 μM O-methyl-l-tyrosinyl-γ-l-glutamyl-l-cysteinylglycine (UPF1) (added at 1h). Long grey dashes: HBEC treated with CSC and 10 μM UPF1 (added at 1h). Short black dashes: HBEC treated with ECL and 2 mM N-acetylcysteine (NAC) (added at 1h). Short grey dashes: HBEC treated with CSC and 2 mM NAC (added at 1h). For each treatment and for any time point, n = 3. Error bars indicate standard errors of means. (A) arginine ([M+H]⁺ = 175); (B) adenosine diphosphate ([M-H]^- = 426); (C) phosphatidylcholine (18:0/20:4) ([M+H]⁺ = 811); (D) α-ketoglutarate ([M-H]^- = 145). *p < 0.05, ECL-exposed cells versus untreated cells; #p < 0.05, ECL- and UPF1-exposed cells versus untreated cells; ^p < 0.05, ECL- and NAC-exposed cells versus untreated cells; ¤p < 0.05, CSC-exposed cells versus untreated cells; ~p < 0.05, CSC- and UPF1-exposed cells versus untreated cells; “p < 0.05, CSC- and NAC-exposed cells versus untreated cells.</p