Botanical and Genetic Identification Followed by Investigation of Chemical Composition and Biological Activities on the Scabiosa atropurpurea L. Stem from Tunisian Flora

Scarce information about the phenolic composition of Scabiosa atropurpurea L. is available, and no carotenoid compounds have been reported thus far. In this study the phenolic and carotenoid composition of this plant was both investigated and associated bioactivities were evaluated. Aiming to obtain extracts and volatile fractions of known medicinal plants to valorize them in the pharmaceutical or food industries, two techniques of extraction and five solvents were used to determine the biologically active compounds. Gas chromatography coupled to flame ionization and mass spectrometry and liquid chromatography coupled to photodiode array and atmospheric pressure chemical ionization/electrospray ionization mass spectrometry highlighted the presence of 15 volatiles, 19 phenolics, and 24 natural pigments in Scabiosa atropurpurea L. stem samples; among them, the most abundant were 1,8-cineole, chlorogenic acid, cynaroside, and lutein. Bioactivity was assessed by a set of in vitro tests checking for antioxidant, antibacterial, antifungal, and allelopathic (against Brassica oleracea L. and Lens culinaris Medik) effects. Scabiosa atropurpurea L. stem extracts presented a considerable antioxidant, antibacterial, and allelopathic potential, with less antifungal effectiveness. These results indicate that the volatile fractions and extracts from S. atropurpurea L. stem could be considered as a good source of bioactive agents, with possible applications in food-related, agriculture, and pharmaceutical fields. Genetic investigations showed 97% of similarity with Scabiosa tschiliensis, also called Japanese Scabiosa

Large-scale participation in policy design: citizen proposals for rural development in Tunisia

International audienceMore and more literature and practice recommend involving the public at the early stages of the policy cycle, i.e. issue identification, definition of the policy objectives and policy design. Policy design involves, among others, identifying solutions, ideas or alternatives which may address the policy objectives. Three main arguments are often put forward to advocate for the involvement of stakeholders, or the public, in policy design: a "user-centered " argument (i.e. for the policy to better meet people's priorities), an innovation argument (i.e. to conceive new solutions) and a collective argument (i.e. to identify collective actions and better tackle environmental problems). However, in both research and practice these arguments have been challenged. Research has insufficiently generated evidence of the influence of large-scale participation in policy design on resulting proposed actions. The objective of this paper is to analyze whether a large-scale participatory process leads to action proposals that fit people's priorities and that are innovative and collective. It draws from a land management and rural development policy design experiment conducted in six vulnerable areas of Tunisia. 4,300 direct participants were involved and 11,583 action proposals were collected. Our results highlight the influence of the local circumstances on innovation and the interest towards collective actions. Our results also show that whether policy design is made individually or in group influences the outcomes. The results also suggest that appropriate facilitation can help fostering more collective and innovative actions. We conclude the paper by opening up the idea of hybridizing policy design methods with methods from political and agricultural sciences in order to better understand the drivers and rationalities behind participants' action proposals