7 research outputs found
Modulation of plasma membrane Ca2-ATPase by neutral Phospholipids: effect of the micelle-vesicle transition and the bilayer thickness
Background: Membrane proteins require phospholipids to be biologically active. Results: An increase of phosphatidylcholine/detergent molar ratio leads to a biphasic behavior of the PMCA Ca2-ATPase activity, whose maximum depends on phosphatidylcholine characteristics. Conclusion: The optimum hydrophobic thickness for PMCA structure and Ca2-ATPase activity is about 24 Ă
. Significance: Differential modulation by neutral phospholipids could be a general mechanism for regulating membrane protein function.Fil: Pignataro, MarĂa Florencia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas; ArgentinaFil: Dodes Traian, MartĂn Miguel. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas; ArgentinaFil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas; ArgentinaFil: Sica, Mauricio Pablo. Comision Nacional de Energia Atomica. Gerencia del ĂĄrea de Seguridad Nuclear y Ambiente. Instituto de EnergĂa y Desarrollo Sustentable. Instituto de EnergĂa y Desarrollo Sustentable - Sede Bariloche; ArgentinaFil: Mangialavori, Irene Cecilia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas; ArgentinaFil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica. Instituto de QuĂmica y FĂsico-QuĂmica BiolĂłgicas; Argentin
Contamination alters the physicochemical and textural characteristics of clays in the sediments of the peri urban reconquista river, affecting the associated indigenous microorganisms
The physicochemical and textural characteristics of river sediments and, essentially, their clays, are at the center of a network of biological and geochemical factors that are mutually modifying. Therefore, the contamination, the characteristics of the clays, and the associated microorganisms strongly influence each other. In this work, sediments from two sites of the urban Reconquista River, near Buenos Aires City, Argentina, exposed to different environmental contexts were characterized. The huge differences in the organic matter content in the vertical profile between both sediments strongly evidenced the polluted status of San Francisco (SF) site as opposed to the Dique Roggero (DR) site. Thorough physicochemical and textural characterization of the sediments and their clay fraction performed by pH, Oxidation-reduction potential (ORP), spectrophotometry, XRD, laser diffraction, N2 adsorptionâdesorption isotherms, EDS, and SEM measurements revealed that organic matter (DR: 41 ± 5 g kgâ1; SF: 150 ± 30 g kgâ1 ) intervened in the retention of heavy metals (DR: 5.6 mg kgâ1 Zn, 7 mg kgâ1 Cu, 3.1 kgâ1 Cr; SF: 240 mg kgâ1 Zn, 60 mg kgâ1 Cu, 270 mg kgâ1 Cr) and affected the level of association and the formation of mineralâorganic aggregates (DR: 15 ± 3 ”m; SF: 23 ± 4 ”m). This can be decisive in the surface interaction required for the establishment of bacterial assemblages, which determine the biogeochemical processes occurring in sediments and have a key role in the fate of contaminants in situ and in the remediation processes that need to be applied to restore the anoxic contaminated sediments.Fil: Tufo, Ana Elisabeth. Universidad Nacional de San MartĂn. Instituto de InvestigaciĂłn e IngenierĂa Ambiental. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de InvestigaciĂłn e IngenierĂa Ambiental; ArgentinaFil: VĂĄzquez, Susana Claudia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de NanobiotecnologĂa. Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica. Instituto de NanobiotecnologĂa; ArgentinaFil: Porzionato, Natalia Florencia. Universidad Nacional de San MartĂn. Instituto de InvestigaciĂłn e IngenierĂa Ambiental. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de InvestigaciĂłn e IngenierĂa Ambiental; ArgentinaFil: Grimolizzi, MarĂa Celeste. Universidad Nacional de San MartĂn. Instituto de InvestigaciĂłn e IngenierĂa Ambiental. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de InvestigaciĂłn e IngenierĂa Ambiental; ArgentinaFil: Prados, Maria Belen. ComisiĂłn Nacional de EnergĂa AtĂłmica. Gerencia del Area de Seguridad Nuclear y Ambiente. Instituto de EnergĂa y Desarrollo Sustentable; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Sica, Mauricio Pablo. ComisiĂłn Nacional de EnergĂa AtĂłmica. Gerencia del Area de Seguridad Nuclear y Ambiente. Instituto de EnergĂa y Desarrollo Sustentable; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Curutchet, Gustavo Andres. Universidad Nacional de San MartĂn. Instituto de InvestigaciĂłn e IngenierĂa Ambiental. - Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. Instituto de InvestigaciĂłn e IngenierĂa Ambiental; Argentin
Registry of neurological manifestations due to coronavirus-19 (COVID-19)
La enfermedad por COVID-19 se ha extendido por el mundo desde diciembre de 2019. Los sĂntomas neurolĂłgicos forman parte de su espectro clĂnico. Objetivo: Conocer las manifestaciones neurolĂłgicas en pacientes infectados por COVID-19 en Argentina. MĂ©todos: Estudio multicĂ©ntrico realizado en adultos, desde mayo de 2020 a enero de 2021, con COVID-19 confirmado y sĂntomas neurolĂłgicos. Se consignaron variables demogrĂĄficas, existencia de comorbilidades sistĂ©micas o neurolĂłgicas, la forma de comienzo de la infecciĂłn, alteraciĂłn en estudios complementarios y el grado de severidad de los sĂntomas neurolĂłgicos. Resultados: Se incluyeron 817 pacientes de todo el paĂs, 52% varones, edad promedio 38 anos. Ë La mayorĂa sin comorbilidades ni patologĂa neurolĂłgica previa. El primer sĂntoma de la infecciĂłn fue neurolĂłgico en 56,2% de los casos, predominando la cefalea (69%), luego anosmia/ageusia (66%). TambiĂ©n se reportaron mialgias (52%), alodinia/hiperalgesia (18%), astenia (6%). Un 3,2% mostrĂł compromiso difuso del SNC como encefalopatĂa o convulsiones. Un 1,7% tuvo complicaciones cerebrovasculares. Los trastornos del suenoË se observaron en 3,2%. Se reportaron seis pacientes con sĂndrome de Guillain-BarrĂ© (GBS), neuropatĂa perifĂ©rica (3,4%), parestesias en lengua (0,6%), hipoacusia (0,4%), plexopatĂa (0,3%). La severidad de sĂntomas neurolĂłgicos se correlacionĂł con la edad y la existencia de comorbilidades. Conclusiones: Nuestros resultados, similares a los de otros paĂses, muestran dos tipos de sĂntomas neurolĂłgicos asociados a COVID-19: algunos potencialmente incapacitantes o mortales como el GBS o la encefalitis, y otros menos devastadores, pero mĂĄs frecuentes, como cefalea o anosmia que demandan en forma creciente atenciĂłn a largo plazo.COVID-19 disease has spread around the world since December 2019. Neurological symptoms are part of its clinical spectrum. Objective: To know the neurological manifestations in patients infected by COVID-19 in Argentina. Methods: Multicenter study conducted in adults, from May 2020 to January 2021, with confirmed COVID-19 and neurological symptoms. Demographic variables, existence of systemic or neurological comorbidities, the form of onset of the infection, alteration in complementary studies and the degree of severity of neurological symptoms were recorded. Results: 817 patients from all over the country were included, 52% male, mean age 38 years, most of them without comorbidities or previous neurological pathology. The first symptom of the infection was neurological in 56.2% of the cases, predominantly headache (69%), then anosmia/ageusia (66%). Myalgias (52%), allodynia/hyperalgesia (18%), and asthenia (6%) were also reported. 3.2% showed diffuse CNS involvement such as encephalopathy or seizures. 1.7% had cerebrovascular complications. Sleep disorders were observed in 3.2%. 6 patients were reported with Guillain BarrĂ© (GBS), peripheral neuropathy (3.4%), tongue paresthesia (0.6%), hearing loss (0.4%), plexopathy (0.3%). The severity of neurological symptoms was correlated with age and the existence of comorbidities. Conclusions: Our results, similar to those of other countries, show two types of neurological symptoms associated with COVID-19: some potentially disabling or fatal such as GBS or encephalitis, and others less devastating, but more frequent such as headache or anosmia that demand increasingly long-term care.Fil: Alessandro, Lucas. FundaciĂłn para la Lucha contra las Enfermedades NeurolĂłgicas de la Infancia; ArgentinaFil: Appiani, Franco. FundaciĂłn Favaloro; ArgentinaFil: Bendersky, Mariana. Universidad de Buenos Aires; ArgentinaFil: Borrego Guerrero, Brenda. Sanatorio Tandil; ArgentinaFil: Bruera, Guadalupe. Hospital Privado de Rosario; ArgentinaFil: Cairola, Patricia. Gobierno de la Ciudad AutĂłnoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Calandri, Ismael. FundaciĂłn para la Lucha contra las Enfermedades NeurolĂłgicas de la Infancia; ArgentinaFil: Cardozo Oliver, Juan MartĂn. Sanatorio Finochietto; ArgentinaFil: ClĂ©ment, MarĂa Emilia. Hospital Privado de la Comunidad; ArgentinaFil: Di Egidio, Marianna. Tornu Hospital; ArgentinaFil: Di Pace, JosĂ© Luis. Gobierno de la Ciudad AutĂłnoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Diaconchuk, Melina Alejandra. Hospital San Luis; ArgentinaFil: Esliman, Celeste. FundaciĂłn para la Lucha contra las Enfermedades NeurolĂłgicas de la Infancia; ArgentinaFil: Esnaola y Rojas, MarĂa Martha. Universidad de Buenos Aires; ArgentinaFil: FernĂĄndez Boccazzi, JuliĂĄn. Sanatorio de la Trinidad; ArgentinaFil: Franco, Andrea Fabiana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos MejĂa"; ArgentinaFil: Gargiulo Monachelli, Gisella Mariana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Parque Centenario. CEMIC-CONICET. Centro de Educaciones MĂ©dicas e Investigaciones ClĂnicas "Norberto Quirno". CEMIC-CONICET; ArgentinaFil: Giardino, Daniela Laura. Centro de EducaciĂłn Medica E Invest.clinicas; ArgentinaFil: GĂłmez, CĂ©sar. No especifĂca;Fil: Guevara, Ana Karina. No especifĂca;Fil: GutiĂ©rrez, Natalia. Sanatorio Julio MĂ©ndez; ArgentinaFil: Hryb, Javier. Gobierno de la Ciudad AutĂłnoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Ibarra, Viviana. Sanatorio Julio MĂ©ndez; ArgentinaFil: Janota, Franco. Universidad de Buenos Aires; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Larcher, Luis Alfredo. Sanatorio del Norte; ArgentinaFil: Leone, Fernando. Centro MĂ©dico Roca; ArgentinaFil: Luetic, Geraldine. No especifĂca;Fil: Medina, Claudia Andrea. Sanatorio Las Lomas; ArgentinaFil: Menichini, MarĂa Laura. No especifĂca;Fil: Nieto, Gonzalo. Hospital General de Agudos Bernardino Rivadavia ; Gobierno de la Ciudad Autonoma de Buenos Aires;Fil: PĂĄez, MarĂa Fernanda. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. FernĂĄndez"; ArgentinaFil: Peñalver, Francisco. No especifĂca;Fil: Perassolo, MĂłnica. Universidad de Buenos Aires; Argentina. Gobierno de la Ciudad AutĂłnoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Persi, Gabriel. Universidad de Buenos Aires; ArgentinaFil: Pestchanker, Claudia. Hospital San Luis; ArgentinaFil: Porta, Oscar. Gobierno de la Ciudad AutĂłnoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Rey, Roberto Daniel. Universidad de Buenos Aires; ArgentinaFil: RodrĂguez, Gabriel Eduardo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos MejĂa"; ArgentinaFil: Romano, Marina. Centro de EducaciĂłn Medica E Invest.clinicas; ArgentinaFil: Rugiero, Marcelo. Hospital Italiano; ArgentinaFil: SaidĂłn, Patricia. Universidad de Buenos Aires; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos MejĂa"; ArgentinaFil: Sica, MarĂa Florencia. Hospital Privado de la Comunidad; ArgentinaFil: Stankievich, Erica. No especifĂca;Fil: Tarulla, Adriana. No especifĂca;Fil: Zalazar, Guillermo. Hospital San Luis; Argentin
Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries
Background
Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks.
Methods
The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned.
Results
A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31).
Conclusion
Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)