Temperature Sensor Denoising Algorithm Based on Curve Fitting and Compound Kalman Filtering

One of the most important ocean water parameters in world ocean observations is temperature. In the application of high-precision ocean sensors, there are often various interferences and random noises. These noises will cause the linearity of the sensor to change, and it is difficult to estimate the statistical characteristics, and the results will deviate from the real temperature. Aiming at the problems in the application, this paper proposes a compound Kalman smoothing filter (CKSF) algorithm based on least square curve fitting. This algorithm first analyzes the system model of the sensor, uses the least square method to fit the theoretical data and eliminate the non-linear factors caused by system itself, then estimates the statistical characteristics of the noise required by modeling, using the wavelet transform method to track the change of noise in real time and to accurately estimate the noise variance. Finally, a compound filtering method including wavelet transform and Kalman smoothing filtering is used as the main denoising algorithm, which is more accurate than a single Kalman filtering result. The algorithm is applied to the temperature measurement process of the ocean temperature sensor. The results show that the accuracy and stability of the sensor are improved

Antiinfective therapy with a small molecule inhibitor of Staphylococcus aureus sortase

International audienceMethicillin-resistant Staphylococcus aureus (MRSA) is the most frequent cause of hospital-acquired infection, which manifests as surgical site infections, bacteremia, and sepsis. Due to drug-resistance, prophylaxis of MRSA infection with antibiotics frequently fails or incites nosocomial diseases such as Clostridium difficile infection. Sortase A is a transpeptidase that anchors surface proteins in the envelope of S. aureus, and sortase mutants are unable to cause bacteremia or sepsis in mice. Here we used virtual screening and optimization of inhibitor structure to identify 3-(4-pyridinyl)-6-(2-sodiumsulfonatephenyl)[1,2,4]triazolo[3,4-b][1,3,4]thiadiazole and related compounds, which block sortase activity in vitro and in vivo. Sortase inhibitors do not affect in vitro staphylococcal growth yet protect mice against lethal S. aureus bacteremia. Thus, sortase inhibitors may be useful as antiinfective therapy to prevent hospital-acquired S. aureus infection in high-risk patients without the side effects of antibiotics

Daily Therapy with a Slow-releasing H2S Donor GYY4137 Enables Early Functional Recovery and Ameliorates Renal Injury Associated with Urinary Obstruction

Objectives: To assess the effects of slow-releasing H2S donor GYY4137 on post-obstructive renal function and injury following unilateral ureteral obstruction (UUO) by using the UUO and reimplantation (UUO-R) model in rats and to elucidate potential mechanisms by using an in vitro model of epithelial-mesenchymal transition (EMT). Methods: Male Lewis rats underwent UUO at the left ureterovesical junction. From post-operative day (POD) 1-13, rats received daily intraperitoneal (IP) injection of phosphate buffered saline (PBS, 1 mL) or GYY4137 (200 mu mol/kg/day in 1 mL PBS, IP). On POD 14, the ureter was reimplanted back into the bladder, followed by a right nephrectomy. Urine and serum samples were collected to monitor renal function. On POD 30, the left kidney was removed and tissue sections were stained with H&E, TUNEL, CD68, CD206, myeloperoxidase, and Masson\u27s trichrome to determine cortical thickness, apoptosis, inflammation, and fibrosis. In our in vitro model of EMT, NRK52E cells were treated with 10 ng/mL TGF-beta 1, 10 mu M GYY4137 and/or 50 mu M GYY4137. Western blot analysis was performed to determine the expression of E-cadherin, vimentin, Smad7 and TGF-beta 1 receptor II (T beta RII). Results: GYY4137 led to a moderate decrease in post-obstructive serum creatinine, cystatin C and FENa. We also observed a trend towards a decrease in post-obstructive proteinuria following GYY4137 treatment. Histologically, we observed a significant decrease in apoptosis, inflammation, and fibrosis. Furthermore, our in vitro studies demonstrate that in the presence of TGF-beta 1, GYY4137 significantly decreases vimentin and T beta RII and significantly increases E-cadherin and Smad7. Conclusions: H2S may help to accelerate the recovery of renal function post-obstruction and attenuates renal injury associated with UUO. It is possible that H2S mitigates fibrosis by regulating the TGF-beta 1-mediated EMT pathway. Taken together, our data suggest that H2S may be a potential novel therapy for improving renal function and limiting renal injury associated with obstructive uropathy