Isolation and characterization of novel phage displayed scFv antibody for human tumor necrosis factor alpha and molecular docking analysis of their interactions

Introduction: Tumor necrosis factor alpha (TNF-α) expression amplifies to excess amounts in several disorders such as rheumatoid arthritis and psoriasis. Although, Anti-TNF biologics have revolutionized the treatment of these autoimmune diseases, formation of anti-drug antibodies (ADA) has dramatically affected their use. The next generation antibodies (e.g. Fab, scFv) have not only reduced resulted immunogenicity, but also proved several benefits including better tumor penetration and more rapid blood clearance.This study highlights the use of phage display for identification of human single chain fragment antibody against disulfide-bonded TNF-α using phage display technology. Methods and Results: Using affinity selection procedures in this study, a scFv antibody clone was isolated from naïve Tomlinson I phage display library that specifically recognizes and binds to TNF-α. The TNF-α recombinant protein was expressed in genetically engineered Escherichia coli SHuffle® T7 Express, for the first time, which is able to express disulfide-bonded recombinant proteins into their correctly folded states. Conclusions: ELISA-based affinity characterization results indicated that the isolated novel 29.2 kDa scFv binds TNF-α with suitable affinity. In silico homology modeling study using ‘ModWeb’ as well as molecular docking study using Hex program confirmed the scFv and TNF-α interactions with a scFv-TNF- α binding energy of around -593 kj/mol which is well in agreement with our ELSIA results. The cloned scFv antibody may potentially be useful for research and therapeutic applications in the future

Participatory Planning: A Strategy for Organizing and Reinventing Urban Worn

The phenomenon of unstable major cities  especially in developing countries, is common in urban species. Although the effects of these settlements dating back to their cell, they are generally a reflection of the shortcomings and failures such as the lack of municipal services and capital, uneven and unhelpful networks and pathways, and their environmental problems . Until now, Governmental and private organizations in different countries have tried to excel and rebuild old and worn parts of a city. But, researches show that the most effective action on excelling and rebuilding worn parts is cooperation and participation of citizens. They can create some organizations and NGOs.  This study shows that citizen participation in development and rebuilding worn parts is in four axels including culture of people, the availability level of Collaborative Management requirements, and the rules and laws governing people's lives. So, in this article, it is tried to make some managing patterns and human participation methods and finding merits and demerits of methods. These actions can be done by studies in libraries and finding some methods is suggested based on true and scientific management. Results indicated that the program of excelling and rebuilding of urban worn parts are one of expanding cities politics. There are lots of capacities on building houses and increasing urban facilities access. But the realization of this program due to its heavy investment requirement, and according to the widely -worn parts can be done only by public participation and public secto

Participatory Planning: A Strategy for Organizing and Reinventing Urban Worn

The phenomenon of unstable major cities  especially in developing countries, is common in urban species. Although the effects of these settlements dating back to their cell, they are generally a reflection of the shortcomings and failures such as the lack of municipal services and capital, uneven and unhelpful networks and pathways, and their environmental problems . Until now, Governmental and private organizations in different countries have tried to excel and rebuild old and worn parts of a city. But, researches show that the most effective action on excelling and rebuilding worn parts is cooperation and participation of citizens. They can create some organizations and NGOs.  This study shows that citizen participation in development and rebuilding worn parts is in four axels including culture of people, the availability level of Collaborative Management requirements, and the rules and laws governing people's lives. So, in this article, it is tried to make some managing patterns and human participation methods and finding merits and demerits of methods. These actions can be done by studies in libraries and finding some methods is suggested based on true and scientific management. Results indicated that the program of excelling and rebuilding of urban worn parts are one of expanding cities politics. There are lots of capacities on building houses and increasing urban facilities access. But the realization of this program due to its heavy investment requirement, and according to the widely -worn parts can be done only by public participation and public secto

Participatory Planning: A Strategy for Organizing and Reinventing Urban Worn

The phenomenon of unstable major cities  especially in developing countries, is common in urban species. Although the effects of these settlements dating back to their cell, they are generally a reflection of the shortcomings and failures such as the lack of municipal services and capital, uneven and unhelpful networks and pathways, and their environmental problems . Until now, Governmental and private organizations in different countries have tried to excel and rebuild old and worn parts of a city. But, researches show that the most effective action on excelling and rebuilding worn parts is cooperation and participation of citizens. They can create some organizations and NGOs.  This study shows that citizen participation in development and rebuilding worn parts is in four axels including culture of people, the availability level of Collaborative Management requirements, and the rules and laws governing people's lives. So, in this article, it is tried to make some managing patterns and human participation methods and finding merits and demerits of methods. These actions can be done by studies in libraries and finding some methods is suggested based on true and scientific management. Results indicated that the program of excelling and rebuilding of urban worn parts are one of expanding cities politics. There are lots of capacities on building houses and increasing urban facilities access. But the realization of this program due to its heavy investment requirement, and according to the widely -worn parts can be done only by public participation and public secto

Quantitative mapping of surface elastic moduli in silica-reinforced rubbers and rubber blends across the length scales by AFM

The surface elastic moduli of silica-reinforced rubbers and rubber blends were investigated by atomic force microscopy (AFM)-based HarmoniX material mapping. Styrene–butadiene rubbers (SBR) and ethylene–propylene–diene rubbers (EPDM) and SBR/EPDM rubber blends with varying concentrations of silica nanoparticles (0, 5, 10, 20, 50 parts per hundred rubber, phr) were prepared to investigate the effect of different composition on the resulting morphology, filler distribution and elastic moduli of a specific rubber or rubber blend sample. For SBR, the elastic modulus values varied from 0.5 MPa for unfilled SBR to 5 MPa for 50 phr reinforced SBR with the increase in the concentration of filler. For EPDM, the corresponding values increased from 1.4 MPa for unfilled EPDM to 4.5 MPa for 50 phr reinforced EPDM. Local stiff and soft domains in silica-reinforced SBR and EPDM rubbers and rubber blends were identified by HarmoniX AFM imaging. While the stiff silica particles show modulus values as high as 2 GPa, the rubber matrix reveals modulus values in the range of ca. 30 MPa for the rubber blends to ca. 300 MPa for the unfilled rubbers. The lower value of elastic modulus of the EPDM phase in the blend, compared to the blank EPDM compound can be attributed to the presence of Sunpar oil in the compound which has a very good affinity with EPDM and decreases the rubber modulus. The elastic moduli maps revealed an increase of the areal fraction of silica particles showing an intrinsic surface modulus value with rising silica content in the compound preparation mixture. HarmoniX AFM measurements revealed the formation of larger silica aggregates in EPDM in contrast to SBR where isolated silica particles were observed. For silica-reinforced rubber blends a phase separation into a soft (ca. 40 MPa) and a significantly harder phase could be observed (ca. 500 MPa–1.5 GPa) indicating the incorporation of silica particles in the SBR phase. Using HarmoniX AFM imaging significantly higher surface elastic moduli were observed compared to those obtained by bulk tensile testing. Possible reasons for the observed differences between bulk modulus values and those measured by AFM are discussed in detail, including the aspect of different averaging procedures like inherent to surface probing by AFM versus bulk tensile testing, different filler distributions in SBR and EPDM and the AFM modulus calibratio

The Effect of Different Mixing Methods on the Properties of Calcium-enriched Mixture Cement: A Systematic Review of in Vitro Studies

Introduction: It has been shown that the mechanical and physical properties of Calcium Enriched Mixture (CEM) cement are influenced by the mixing methods. Despite several studies conducted on different mixing methods of CEM cement, there is no systematic review to summarize the results. This systematic review was conducted to investigate the effect of different mixing techniques on mechanical and physical characteristics of CEM cement. Methods and Materials: A professional librarian with skills in informatics conducted a systematic search by searching electronic databases PubMed/MEDLINE and Scopus and Ovid for English language peer-reviewed articles published between 1992 and April 2019. Results: Initial searches from all sources identified 1175 references. Two of the authors examined the titles, abstracts of these articles and the full reports of 20 studies were obtained, and data extraction was performed. Seven studies satisfied the eligibility criteria for the review. The effect of different mixing methods was investigated on bacterial microleakage, push-out bond strength, flow rate, compressive strength, solubility, pH, film thickness, dimensional changes, working time, setting time and quality of the apical plug. Conclusion: Based on the results of this systematic review, some of the important properties of CEM cement were affected by different mixing methods. Although none of these mixing methods could improve all the properties, mechanical and manual methods were more effective compared to ultrasonic method.  Keywords: Calcium-enriched Mixture Cement; Systematic Review; Ultrasoni

The diagnostic value of macrophage migration inhibitory factor, carcinoembryonic antigen, and carbohydrate antigen 19-9 in gastric cancer

Introduction: Gastric cancer is a rather common malignancy worldwide and a major healthcare system issue. Lately, the importance of biomarkers such as macrophage migration inhibitory factor (MIF) has been demonstrated in the diagnosis of various gastrointestinal (GI) malignancies. The present study aimed to evaluate the diagnostic value of MIF, carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA 19-9) in gastric cancer. Methods: In this descriptive-analytical study, 84 patients with gastric cancer referred to the gastroenterology clinic of Tabriz University of Medical Sciences, Tabriz, Iran, for diagnostic and therapeutic procedures, and 80 healthy individuals were enrolled. Serum levels of MIF, CEA, and CA 19-9 were measured in both groups. Further, the grade and stage of the cancer were determined in in the patient group. Results: Serum levels of all three MIF, CEA, and CA 19-9 biomarkers in patients with gastric cancer were significantly higher than those of the control group (P = 0.001). However, no statistically significant correlations were found between the studied biomarkers with the tumor grade and stage. The MIF cut-off point for the diagnosis of gastric cancer was found to be 7.05 pg/ml and its sensitivity and specificity were 85.7% and 73.8%, respectively. Conclusion: MIF biomarker may involve in the pathogenesis and development of gastric cancer and it is a potential diagnostic and therapeutic marker in this malignancy

Presenting a New Method for Link Prediction in Social Networks

Today, online social networks are very popular due to the possibility of creating relationships between people all over the world. These social networks with possibilities such as friend recommendation generally use local features derived from social graph structure. For friend recommendation, there are different algorithms with local and global approaches. In this paper, we proposed an algorithm with local approach that has a suitable performance compared to other algorithms. In addition, it has an acceptable speed, because of its being local. This new feature was examined on two large social networks: Epinions and Facebook. The research showed that this algorithm can present good predictions and acceptable recommendations