Comparative Biochemistry of Hemoglobins

To compare Macaca mulatta monkey hemoglobin with human hemoglobin, these two varieties of hemoglobin were examined by means of agar-gel electrophoresis, cellulose acetate membrane electrophoresis, column chromatography on CM-cellulose, and the alkali denaturation method. In addition, these hemoglobins were compared by the fingerprinting method on their tryptic digests. As the results, a clear, though slight, difference was observed between these two kinds of hemoglobin

Hypocalcemic Action of a Pancreatic Factor and its Clinical Significance on the Myasthenic Patients

As reported in the previous short communication1), the authors have been engaged in isolating a protein anabolic factor from hog pancreas for the last eighteen years. The extraction procedure of fraction B reported herein, which is more effective against 15 myasthenic patients than any other drugs, has been established for the last five years

Adsorption Chromatography of Dinitrophenylamino Acids on Silica Gel-Celite

The technique of adsorption chromatography of dinitrophenylamino acids (DNP-amino acids) has been studied in this laboratory in order to separate N-terminal amino acids from the globins of various hemoglobins. The following method which gave good results is a modified method of GREEN et al. A mixture of silica gel and celite (2 to 1) is packed into a 15-cm. column with diameter of 0.9 cm. As developers mixtures of organic solvents such as acetic acid, acetone, ligroin, formic acid, ethyl acetate or benzene are used. In the above column and with the above developers, adsorption chromatography is carried out to separate various DNP-amino acids. This seems to be an effective method for qualitative and quantitative analysis of DNA-amino acids

Comparative Biochemistry of Hemoglobins

The problem of the molecular evolution of hemoglobin has been studied by comparing agar-gel electrophoresis, ultraviolet absorption spectra and resistance to alkalis in fifteen varieties of hemoglobins ranging from human hemoglobin to fish hemoglobin. The differences observed in the hemoglobins seem to be due to differences in the structures of these hemoglobins

A Pancreatic Protein Anabolic Extract

The pancreatic extract which was isolated by our procedure was a protein. The extract exhibited some protein anabolic action, and it was effective in several patients with myasthenia gravis and aplastic anemia