Fabrication and Characterization of Electrospun Drug-eluting Nanofibers from Polycaprolactone/Chitosan Blends

Electrospinning has emerged as a widely accepted technique with ability to produce nanofibers that can be employed in many biomedical applications. In particular, drug-eluting nanofibers have become very popular in controlled release of small molecule drugs. In this study, nanofibers from blends of polylactocaprone (PCL) and chitosan (CHI) were electrospun with the ability to load a model drug, acetylsalicylic acid (ASA), at 10 wt%. PCL/CHI fibers exhibited smooth surface morphology at polymer compositions ranging from 100/0 to 40/60 with or without the incorporation of ASA. Mechanical properties suggested a brittle failure mechanism for fibers loaded with drug. In vitro drug release study displayed a controlled release profile of ASA up to 48 h. Our study aims to explore the drug-polymer interactions and their effects on fiber structure, mechanical properties and drug release profile.Cockrell School of Engineerin

Design and Experimental Analysis of Ventilated Walls and "Ice House" Roofs Applications in Warm Climates

This paper contains the findings of experimental research conducted to determine the effectiveness of ventilated walls and "ice house" roof applications in hot-humid climates. Ventilated wall and "ice house" roof is the type of construction which consists of interposing an additional wall or roof skin between the standard building envelope and the exterior environment. The new skin is separated from the building envelope by an air space, which is usually vented to the ambient environment. The primary objective of such construction is to eliminate or drastically reduce the effects of solar loading on the building envelope. The information presented in this paper can enable the designer to have a better understanding of how buildings might function at various times of the day and the season. Recommendations on applications of new buildings and retrofit of existing structures are presented here as well

Identification and characterization of novel human tissue-specific RFX transcription factors

<p>Abstract</p> <p>Background</p> <p>Five regulatory factor X (RFX) transcription factors (TFs)–RFX1-5–have been previously characterized in the human genome, which have been demonstrated to be critical for development and are associated with an expanding list of serious human disease conditions including major histocompatibility (MHC) class II deficiency and ciliaophathies.</p> <p>Results</p> <p>In this study, we have identified two additional RFX genes–RFX6 and RFX7–in the current human genome sequences. Both RFX6 and RFX7 are demonstrated to be winged-helix TFs and have well conserved RFX DNA binding domains (DBDs), which are also found in winged-helix TFs RFX1-5. Phylogenetic analysis suggests that the RFX family in the human genome has undergone at least three gene duplications in evolution and the seven human RFX genes can be clearly categorized into three subgroups: (1) RFX1-3, (2) RFX4 and RFX6, and (3) RFX5 and RFX7. Our functional genomics analysis suggests that RFX6 and RFX7 have distinct expression profiles. RFX6 is expressed almost exclusively in the pancreatic islets, while RFX7 has high ubiquitous expression in nearly all tissues examined, particularly in various brain tissues.</p> <p>Conclusion</p> <p>The identification and further characterization of these two novel RFX genes hold promise for gaining critical insight into development and many disease conditions in mammals, potentially leading to identification of disease genes and biomarkers.</p

Measurement Error in Job Evaluation and the Gender Wage Gap

Job evaluation is used to establish pay for many workers in the United States and has been used to measure the extent of pay discrimination. However, job evaluations are subject to measurement error that can bias these estimates. Using computed reliability ratios to adjust for measurement error in a study of Iowa state government jobs, we find that measurement errors exaggerate the implied extent of discrimination against predominantly female jobs by 34%-44%. Measurement errors also exaggerate the number of independent job factors which affect pay. A practical procedure for making these corrections is illustrated

Isomeric triazines exhibit unique profiles of bioorthogonal reactivity.

Expanding the scope of bioorthogonal reactivity requires access to new and mutually compatible reagents. We report here that 1,2,4-triazines can be tuned to exhibit unique reaction profiles with biocompatible strained alkenes and alkynes. Computational analyses were used to identify candidate orthogonal reactions, and the predictions were experimentally verified. Notably, 5-substituted triazines, unlike their 6-substituted counterparts, undergo rapid [4 + 2] cycloadditions with a sterically encumbered strained alkyne. This unique, sterically controlled reactivity was exploited for dual bioorthogonal labeling. Mutually orthogonal triazines and cycloaddition chemistries will enable new multi-component imaging applications

Control of RelB during dendritic cell activation integrates canonical and noncanonical NF-κB pathways.

The NF-κB protein RelB controls dendritic cell (DC) maturation and may be targeted therapeutically to manipulate T cell responses in disease. Here we report that RelB promoted DC activation not as the expected RelB-p52 effector of the noncanonical NF-κB pathway, but as a RelB-p50 dimer regulated by canonical IκBs, IκBα and IκBɛ. IκB control of RelB minimized spontaneous maturation but enabled rapid pathogen-responsive maturation. Computational modeling of the NF-κB signaling module identified control points of this unexpected cell type-specific regulation. Fibroblasts that we engineered accordingly showed DC-like RelB control. Canonical pathway control of RelB regulated pathogen-responsive gene expression programs. This work illustrates the potential utility of systems analyses in guiding the development of combination therapeutics for modulating DC-dependent T cell responses

Radiation-Induced Large Vessel Cerebral Vasculopathy in Pediatric Patients with Brain Tumors Treated with Proton Radiotherapy

Purpose The purpose of this research is to evaluate the incidence, time to development, imaging patterns, risk factors, and clinical significance of large vessel cerebral vasculopathy in pediatric patients with brain tumors treated with proton radiotherapy. Materials and Methods A retrospective study was performed on 75 consecutive pediatric patients with primary brain tumors treated with proton radiotherapy. Radiation-induced large vessel cerebral vasculopathy (RLVCV) was defined as intracranial large vessel arterial stenosis or occlusion confirmed on MRA, CTA, and/or catheter angiography within an anatomic region with previous exposure to proton beam therapy and not present prior to radiotherapy. Clinical records were used to determine the incidence, timing, radiation dose to the large vessels, and clinical significance associated with the development of large vessel vasculopathy in these patients. Results RLVCV was present in 5/75 (6.7%) of patients and included tumor pathologies of craniopharyngioma (2), ATRT (1), medulloblastoma (1), and anaplastic astrocytoma (1). Median time from completion of radiotherapy to development was 1.5 years (mean 3.0 years; range 1.0-7.5 years). Neither mean age at time of radiotherapy (5.1 years) nor mean radiotherapy dose to the large vessels (54.5 Gy) were statistically significant risk factors. Four of the five patients with RLVCV presented with acute stroke, and demonstrated MRI evidence of acute infarcts in the expected vascular distributions. Angiography studies demonstrated collateral vessel formation in only two of the patients with RLVCV. No patients demonstrated acute hemorrhage or aneurysm. Two patients were treated with pial synangiomatosis surgery. Conclusion RLVCV can occur in pediatric patients with brain tumors treated with proton radiotherapy. Further studies are necessary to determine potential risk factors for large vessel vasculopathy with proton radiotherapy in comparison with conventional photon radiotherapy

Vortex Structures in YBa₂Cu₃O₇ (Invited)

Extensive small angle neutron scattering experiments have been conducted on the vortex system in YBa2Cu3O7 in a magnetic field range of 0.5 T≤H≤5 T, and with various orientations of the magnetic field with respect to the crystallographic axes. For H parallel to the c axis, the vortex lattice is oblique with two nearly equal lattice constants and an angle of 73°between primitive vectors. One principal axis of the vortex lattice coincides with the (110) direction of the crystal lattice. It is shown that this structure cannot be explained in the framework of a purely electrodynamic (London) model, and that it is intimately related to the in-plane anisotropy of the superconducting coherence length. When the field is inclined with respect to the c axis, the uniaxial anisotropy due to the layered crystal structure of YBa2Cu3O7 becomes relevant. The interplay between the square in-plane anisotropy and the uniaxial anisotropy leads to both a continous structural transition and a reorientation of the vortex lattice as a function of inclination angle. For the largest inclination angles, the vortex lattice decomposes into independent chains

Vortex Lattice Symmetry and Electronic Structure in YBa₂Cu₃O₇

We report a small angle neutron scattering study of the vortex lattice in YBa2Cu3O7 in magnetic fields of 0.5≤H≤5 T applied along and close to the c axis. Over the entire field range, the vortices form an oblique lattice with two nearly equal lattice constants and an angle of 73°between primitive vectors. Numerical calculations suggest that variations of the superconducting order parameter near the vortex core are important in stabilizing this structure. An analysis that accounts for the fourfold symmetry of the vortex core qualitatively explains both the symmetry and the orientation of the observed vortex lattice. A quantitative explanation of our data will require calculations based on a realistic gap equation