29 research outputs found
Resonance fluorescence from a telecom-wavelength quantum dot
© 2016 Author(s).We report on resonance fluorescence from a single quantum dot emitting at telecom wavelengths. We perform high-resolution spectroscopy and observe the Mollow triplet in the Rabi regime - a hallmark of resonance fluorescence. The measured resonance-fluorescence spectra allow us to rule out pure dephasing as a significant decoherence mechanism in these quantum dots. Combined with numerical simulations, the experimental results provide robust characterisation of charge noise in the environment of the quantum dot. Resonant control of the quantum dot opens up new possibilities for the on-demand generation of indistinguishable single photons at telecom wavelengths as well as quantum optics experiments and direct manipulation of solid-state qubits in telecom-wavelength quantum dots
Níveis de Proteína Bruta da Ração para Marrãs em Gestação Levels of Crude Protein of the Ration for Gilts in Gestation
Foram utilizadas 50 marrãs mestiças (Landrace, Large White e Pietrain) com média de peso de 136,34 ±16,05 kg e idade média de 220 dias, respectivamente, para avaliar diferentes níveis de proteína bruta da ração desses animais. Foi empregado o delineamento de blocos ao acaso, com cinco tratamento, 10 repetições e um animal por unidade experimental. Os tratamentos corresponderam a rações com 10,0; 11,5; 13,0; 14,5; e 16,0% de proteína bruta. O ganho de peso das marrãs até os 90 dias de gestação e o peso dos leitões e da leitegada se elevaram de forma linear com o aumento do nível de proteína da ração. Houve efeito quadrático dos níveis de proteína sobre o consumo da porca na lactação, que aumentou até o nível de 12,96% de PB. Não se verificou efeito dos tratamentos sobre o número de leitões nascidos, o número de dias desmame-cio e sobre o ganho de peso da porca durante todo o ciclo reprodutivo. Entretanto, houve efeito quadrático sobre o número de leitões desmamados, que aumentou até o nível de 13,33% de PB. Os níveis de proteína bruta que propiciaram menor taxa de mortalidade dos leitões na fase de lactação situaram-se entre 11,5 e 16% de PB. O melhor nível de proteína bruta nas dietas no período de gestação para marrãs pesando 136,34 ±16,05 kg e idade média de 220 dias foi de 13,31%, correspondendo a um consumo estimado de 240 g de proteína/dia ou 0,67% de lisina (12,06 g/dia), respectivamente.<br>Fifty crossbreed gilts (Landrace, Large White and Pietrain) with 136.34±16.05 and 220 days of average weight and age, respectively, were used to evaluate diets with different crude protein levels, at the gestation period. A complete randomized blocks design, with five treatments, ten replicates and one animal per experimental unit, was used. The treatments corresponded to the diets with 10.0, 11.5, 13.0, 14.5 and 16.0% of crude protein. The weight gains of the gilts at the 90 days of the gestation period and the individual and total weight of the pigs and litter linearly increased in function of the crescent crude protein level in the diets. There was quadratic effect of the crude protein levels in the diets on the gilts dry matter consumption in the subsequent lactation period. This may be a consequence response of the crude protein level in the diets fed to the gilts at the gestation period. The highest calculated dry matter consumption was at 12.96% of crude protein. No effect of the treatments on the number of weaned pigs was observed, but it increased up to the level of 13.33% of crude protein. A linear decreasing effect was observed on the relative weight of the gilts at the weaning time of the pigs also in function of the crescent crude protein levels in the diets at the gestation period. The best crude protein level in the diets at the gestation period for gilts weighting 136,34±16.05 and with 220 days of age was 13.31% crude protein, corresponding to a dry matter consumption of 240 g of protein/day or 0.67% of lysine (12.06 g/day)
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A peptidomimetic modulator of the CaV2.2 N-type calcium channel for chronic pain
Transmembrane Cav2.2 (N-type) voltage-gated calcium channels are genetically and pharmacologically validated, clinically relevant pain targets. Clinical block of Cav2.2 (e.g., with Prialt/Ziconotide) or indirect modulation [e.g., with gabapentinoids such as Gabapentin (GBP)] mitigates chronic pain but is encumbered by side effects and abuse liability. The cytosolic auxiliary subunit collapsin response mediator protein 2 (CRMP2) targets Cav2.2 to the sensory neuron membrane and regulates their function via an intrinsically disordered motif. A CRMP2-derived peptide (CBD3) uncouples the Cav2.2-CRMP2 interaction to inhibit calcium influx, transmitter release, and pain. We developed and applied a molecular dynamics approach to identify the A1R2 dipeptide in CBD3 as the anchoring Cav2.2 motif and designed pharmacophore models to screen 27 million compounds on the open-access server ZincPharmer. Of 200 curated hits, 77 compounds were assessed using depolarization-evoked calcium influx in rat dorsal root ganglion neurons. Nine small molecules were tested electrophysiologically, while one (CBD3063) was also evaluated biochemically and behaviorally. CBD3063 uncoupled Cav2.2 from CRMP2, reduced membrane Cav2.2 expression and Ca2+ currents, decreased neurotransmission, reduced fiber photometry-based calcium responses in response to mechanical stimulation, and reversed neuropathic and inflammatory pain across sexes in two different species without changes in sensory, sedative, depressive, and cognitive behaviors. CBD3063 is a selective, first-in-class, CRMP2-based peptidomimetic small molecule, which allosterically regulates Cav2.2 to achieve analgesia and pain relief without negative side effect profiles. In summary, CBD3063 could potentially be a more effective alternative to GBP for pain relief.Open access articleThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]