Analysis of Physical Activity and Sedentary Behavior of Chinese Workers in Different Occupations.

departmental bulletin pape

A pH Monitoring Algorithm for Orifice Plate Culture Medium

Recently, there has been renewed interest in cell therapy, which plays a key role in the clinical research of genetic diseases, advanced blood disease, and other diseases. It shows considerable clinical application value and is known as “the new pillar of future medicine”. Automatic cell culture and operation technology is the key to ensuring scale, standardization, and stability between batches of therapeutic cells. The pH of the cell culture medium is vital for cell growth. Most cells are suitable for growth at pH 7.2~7.4. A pH of cell culture medium lower than 6.8 or higher than 7.6 is harmful to cells, and cells will degenerate or even die. At present, the monitoring method of cell culture medium pH of automatic cell culture equipment is mainly a visual observation method, which can not accurately or quickly reflect changes in the cell culture medium. To address the issue of monitoring of cell culture fluid pH for automated cell culture equipment and the inability to employ invasive sensors to measure pH during well plate culture, a pH monitoring method for orifice plate culture medium algorithm based on HSV (hue, saturation, value) model is proposed by studying the changes of cell culture medium in the process of cell culture. The research presented here reveals the laws of cell culture fluid pH change and its color moment, and the intelligent monitoring of cell culture fluid pH was successfully achieved. The problem of non-destructive monitoring of the pH of cell culture fluids in well plates is also addressed

Glioblastoma stem cell-derived exosomal miR-374b-3p promotes tumor angiogenesis and progression through inducing M2 macrophages polarization

Summary: Glioblastoma stem cells (GSCs) reside in hypoxic periarteriolar niches of glioblastoma micro-environment, however, the crosstalk of GSCs with macrophages on regulating tumor angiogenesis and progression are not fully elucidated. GSCs-derived exosomes (GSCs-exos) are essential mediators during tumor immune-microenvironment remodeling initiated by GSCs, resulting in M2 polarization of tumor-associated macrophages (TAMs) as we reported previously. Our data disclosed aberrant upregulation of miR-374b-3p in both clinical glioblastoma specimens and human cell lines of GSCs. MiR-374b-3p level was high in GSCs-exos and can be internalized by macrophages. Mechanistically, GSCs exosomal miR-374b-3p induced M2 polarization of macrophages by downregulating phosphatase and tensin expression, thereby promoting migration and tube formation of vascular endothelial cells after coculture with M2 macrophages. Cumulatively, these data indicated that GSCs exosomal miR-374b-3p can enhance tumor angiogenesis by inducing M2 polarization of macrophages, as well as promote malignant progression of glioblastoma. Targeting exosomal miR-374b-3p may serve as a potential target against glioblastoma