Hydrology, Water Chemistry and Ecological Relations in the Raised Mound of Cowles Bog

The Cowles Bog National Natural Landmark and the wetlands between the dunes near the south shore of Lake Michigan, in Indiana, contain plant species that are typical of circum-neutral fens. The distribution of eight, rather sharply delineated, vegetation types correlates most strongly with water level variations resulting from the presence of a 4.1-ha convex peat mound. A network of shallow ground-water wells installed in the wetland has identified an upwelling of water under artesian pressure at sites underlying the mound. The well-buffered water, containing high concentrations of inorganic solutes, is derived from an aquifer that is recharged on an upland moraine and is confined beneath a clay till sheet. A breach in this clay layer beneath the mound allows water to flow upward and radially outward as the hydraulic head is dissipated in the overlying marl and peat. The marl and organic lake sediments in the wetland were formed during the Nipissing level of ancestral Lake Michigan (4000-6000 years ago) when the wetland basin was probably a small bay of the lake. The peat mound developed when the lake level fell from the Algoma through to modern times. This increased the difference in hydraulic head and increased spring flows, which in turn induced peat formation

Interactions Between Ground Water and Wetlands, Southern Shore of Lake Michigan, USA

Wetlands between, and within, dune-beach complexes along the south shore of Lake Michigan are strongly affected by ground water. The hydrogeology of the glacial drift aquifer system in a 26 km2 area was investigated to determine the effects of ground water on the hydrology and hydrochemistry of Cowles Bog and its adjacent wetlands. The investigation showed that ground water from intermediate- and regional-scale flow systems discharges to Cowles Bog from confined aquifers that underlie the wetland. These flow systems are recharged in moraines south of the dune- beach complexes. Water from the confined aquifers discharges into the surficial aquifer mainly by upward leakage through a buried till sheet that serves as the confining layer. However, the till sheet is breached below a raised peat mound in Cowles Bog, allowing direct upward discharge from the confined aquifer into the surficial sand, marl, and peat. The shallow ground and wetland water in the area influenced by this leakage is a calcium magnesium bicarbonate type, with low tritium concentrations consistent with mixing of older ground water and more recent precipitation. Ground water and wetland water from surrounding areas are less mineralized and have higher tritium concentrations characteristic of precipitation in the late 1970s. The results of this study suggest that wetlands in complex hydrogeologic settings may be influenced by multiple ground-water flow systems that are affected by geomorphic features, stratigraphic discontinuities, and changes in sediment types. Discharge and recharge zones may both occur in the same wetland. Multidisciplinary studies incorporating hydrological, hydrochemical, geophysical, and sedimentological data are necessary to identify such complexities in wetland hydrology

Altered Immune Responses in Rhesus Macaques Co-Infected with SIV and Plasmodium cynomolgi: An Animal Model for Coincident AIDS and Relapsing Malaria

BACKGROUND:Dual epidemics of the malaria parasite Plasmodium and HIV-1 in sub-Saharan Africa and Asia present a significant risk for co-infection in these overlapping endemic regions. Recent studies of HIV/Plasmodium falciparum co-infection have reported significant interactions of these pathogens, including more rapid CD4+ T cell loss, increased viral load, increased immunosuppression, and increased episodes of clinical malaria. Here, we describe a novel rhesus macaque model for co-infection that supports and expands upon findings in human co-infection studies and can be used to identify interactions between these two pathogens. METHODOLOGY/PRINCIPAL FINDINGS:Five rhesus macaques were infected with P. cynomolgi and, following three parasite relapses, with SIV. Compared to macaques infected with SIV alone, co-infected animals had, as a group, decreased survival time and more rapid declines in markers for SIV progression, including peripheral CD4+ T cells and CD4+/CD8+ T cell ratios. The naïve CD4+ T cell pool of the co-infected animals was depleted more rapidly than animals infected with SIV alone. The co-infected animals also failed to generate proliferative responses to parasitemia by CD4+ and CD8+ T cells as well as B cells while also having a less robust anti-parasite and altered anti-SIV antibody response. CONCLUSIONS/SIGNIFICANCE:These data suggest that infection with both SIV and Plasmodium enhances SIV-induced disease progression and impairs the anti-Plasmodium immune response. These data support findings in HIV/Plasmodium co-infection studies. This animal model can be used to further define impacts of lentivirus and Plasmodium co-infection and guide public health and therapeutic interventions

Gene duplication and fragmentation in the zebra finch major histocompatibility complex

BACKGROUND: Due to its high polymorphism and importance for disease resistance, the major histocompatibility complex (MHC) has been an important focus of many vertebrate genome projects. Avian MHC organization is of particular interest because the chicken Gallus gallus, the avian species with the best characterized MHC, possesses a highly streamlined minimal essential MHC, which is linked to resistance against specific pathogens. It remains unclear the extent to which this organization describes the situation in other birds and whether it represents a derived or ancestral condition. The sequencing of the zebra finch Taeniopygia guttata genome, in combination with targeted bacterial artificial chromosome (BAC) sequencing, has allowed us to characterize an MHC from a highly divergent and diverse avian lineage, the passerines. RESULTS: The zebra finch MHC exhibits a complex structure and history involving gene duplication and fragmentation. The zebra finch MHC includes multiple Class I and Class II genes, some of which appear to be pseudogenes, and spans a much more extensive genomic region than the chicken MHC, as evidenced by the presence of MHC genes on each of seven BACs spanning 739 kb. Cytogenetic (FISH) evidence and the genome assembly itself place core MHC genes on as many as four chromosomes with TAP and Class I genes mapping to different chromosomes. MHC Class II regions are further characterized by high endogenous retroviral content. Lastly, we find strong evidence of selection acting on sites within passerine MHC Class I and Class II genes. CONCLUSION: The zebra finch MHC differs markedly from that of the chicken, the only other bird species with a complete genome sequence. The apparent lack of synteny between TAP and the expressed MHC Class I locus is in fact reminiscent of a pattern seen in some mammalian lineages and may represent convergent evolution. Our analyses of the zebra finch MHC suggest a complex history involving chromosomal fission, gene duplication and translocation in the history of the MHC in birds, and highlight striking differences in MHC structure and organization among avian lineages

Long-Term Programming of Antigen-Specific Immunity from Gene Expression Signatures in the PBMC of Rhesus Macaques Immunized with an SIV DNA Vaccine

While HIV-1-specific cellular immunity is thought to be critical for the suppression of viral replication, the correlates of protection have not yet been determined. Rhesus macaques (RM) are an important animal model for the study and development of vaccines against HIV/AIDS. Our laboratory has helped to develop and study DNA-based vaccines in which recent technological advances, including genetic optimization and in vivo electroporation (EP), have helped to dramatically boost their immunogenicity. In this study, RMs were immunized with a DNA vaccine including individual plasmids encoding SIV gag, env, and pol alone, or in combination with a molecular adjuvant, plasmid DNA expressing the chemokine ligand 5 (RANTES), followed by EP. Along with standard immunological assays, flow-based activation analysis without ex vivo restimulation and high-throughput gene expression analysis was performed. Strong cellular immunity was induced by vaccination which was supported by all assays including PBMC microarray analysis that identified the up-regulation of 563 gene sequences including those involved in interferon signaling. Furthermore, 699 gene sequences were differentially regulated in these groups at peak viremia following SIVmac251 challenge. We observed that the RANTES-adjuvanted animals were significantly better at suppressing viral replication during chronic infection and exhibited a distinct pattern of gene expression which included immune cell-trafficking and cell cycle genes. Furthermore, a greater percentage of vaccine-induced central memory CD8+ T-cells capable of an activated phenotype were detected in these animals as measured by activation analysis. Thus, co-immunization with the RANTES molecular adjuvant followed by EP led to the generation of cellular immunity that was transcriptionally distinct and had a greater protective efficacy than its DNA alone counterpart. Furthermore, activation analysis and high-throughput gene expression data may provide better insight into mechanisms of viral control than may be observed using standard immunological assays

The western painted turtle genome, a model for the evolution of extreme physiological adaptations in a slowly evolving lineage

Background: We describe the genome of the western painted turtle, Chrysemys picta bellii, one of the most widespread, abundant, and well-studied turtles. We place the genome into a comparative evolutionary context, and focus on genomic features associated with tooth loss, immune function, longevity, sex differentiation and determination, and the species' physiological capacities to withstand extreme anoxia and tissue freezing.Results: Our phylogenetic analyses confirm that turtles are the sister group to living archosaurs, and demonstrate an extraordinarily slow rate of sequence evolution in the painted turtle. The ability of the painted turtle to withstand complete anoxia and partial freezing appears to be associated with common vertebrate gene networks, and we identify candidate genes for future functional analyses. Tooth loss shares a common pattern of pseudogenization and degradation of tooth-specific genes with birds, although the rate of accumulation of mutations is much slower in the painted turtle. Genes associated with sex differentiation generally reflect phylogeny rather than convergence in sex determination functionality. Among gene families that demonstrate exceptional expansions or show signatures of strong natural selection, immune function and musculoskeletal patterning genes are consistently over-represented.Conclusions: Our comparative genomic analyses indicate that common vertebrate regulatory networks, some of which have analogs in human diseases, are often involved in the western painted turtle's extraordinary physiological capacities. As these regulatory pathways are analyzed at the functional level, the painted turtle may offer important insights into the management of a number of human health disorders

Mineralogy, petrology, and geochemistry of a fassaite-bearing tactite near Helena, Montana

Master of ScienceGeologyUniversity of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/113923/1/39015003271718.pd

Shallow ground-water flow, water levels, and quality of water, 1980-84, Cowles Unit, Indiana Dunes National Lakeshore /

"January 1986."Bibliography: p. 25.Mode of access: Internet

Saline water at the base of the glacial-outwash aquifer near Vincennes, Knox County, Indiana /

"August 1980."Bibliography: p. 53-54.Mode of access: Internet