3D Culturing of Stem Cells: An Emerging Technique for Advancing Fundamental Research in Regenerative Medicine

Regenerative medicine has been coming into spotlight ever since the realisation that conventional treatments are not enough, and the need for specific therapies has emerged. This, however, has paved way for cell-free therapy using extracellular vesicles. A two-dimensional (2D) cell culture model is widely recognised as the “gold standard” for researching cellular communications ex vivo. Although the 2D culture technique is straightforward and easy to use, it cannot replicate the in vivo ECM interactions & microenvironment. On the contrary, 3D culture culturing technology has emerged which include structures such as spheroids and organoids. Organoids are small replicas of in vivo tissues and organs, which faithfully recreate their structures and functions. These could be used as models to derive stem cells based EVs for manufacturing purposes. The linkages between infection and cancer growth, as well as mutation and carcinogenesis, may be modelled using this bioengineered platform. All in all, 3D culturing derived EVs serves as a novel platform for diagnostics, drug discovery & delivery, and therapy

GROWTH on S190814bv: Deep Synoptic Limits on the Optical/Near-Infrared Counterpart to a Neutron Star-Black Hole Merger

On 2019 August 14, the Advanced LIGO and Virgo interferometers detected the high-significance gravitational wave (GW) signal S190814bv. The GW data indicated that the event resulted from a neutron star–black hole (NSBH) merger, or potentially a low-mass binary BH merger. Due to the low false-alarm rate and the precise localization (23 deg2 at 90%), S190814bv presented the community with the best opportunity yet to directly observe an optical/near-infrared counterpart to an NSBH merger. To search for potential counterparts, the GROWTH Collaboration performed real-time image subtraction on six nights of public Dark Energy Camera images acquired in the 3 weeks following the merger, covering >98% of the localization probability. Using a worldwide network of follow-up facilities, we systematically undertook spectroscopy and imaging of optical counterpart candidates. Combining these data with a photometric redshift catalog, we ruled out each candidate as the counterpart to S190814bv and placed deep, uniform limits on the optical emission associated with S190814bv. For the nearest consistent GW distance, radiative transfer simulations of NSBH mergers constrain the ejecta mass of S190814bv to be M_(ej) < 0.04 M⊙ at polar viewing angles, or M_(ej) < 0.03 M⊙ if the opacity is κ < 2 cm²g⁻¹. Assuming a tidal deformability for the NS at the high end of the range compatible with GW170817 results, our limits would constrain the BH spin component aligned with the orbital momentum to be χ < 0.7 for mass ratios Q < 6, with weaker constraints for more compact NSs

The first systematically identified repeating partial tidal disruption event

Tidal disruption events (TDEs) occur when a star enters the tidal radius of a supermassive black hole (SMBH). If the star only grazes the tidal radius, a fraction of the stellar mass will be accreted in a partial TDE (pTDE). The remainder can continue orbiting and may re-disrupted at pericenter, causing a repeating pTDE. pTDEs may be as or more common than full TDEs (fTDEs), yet few are known. In this work, we present the discovery of the first repeating pTDE from a systematically-selected sample, AT\,2020vdq. AT\,2020vdq was originally identified as an optically- and radio-flaring TDE. Around $3$ years after its discovery, it rebrightened dramatically and rapidly in the optical. The optical flare was remarkably fast and luminous compared to previous TDEs. It was accompanied by extremely broad (${\sim}0.1c$) optical/UV spectral features and faint X-ray emission ($L_X \sim 3\times10^{41}$\,erg\,s$^{-1}$), but no new radio-emitting component. Based on the transient optical/UV spectral features and the broadband light curve, we show that AT\,2020vdq is a repeating pTDE. We then use it to constrain TDE models; in particular, we favor a star originally in a very tight binary system that is tidally broken apart by the Hills mechanism. We also constrain the repeating pTDE rate to be $10^{-6}$ to $10^{-5}$ yr$^{-1}$ galaxy$^{-1}$, with uncertainties dominated by the unknown distribution of pTDE repeat timescales. In the Hills framework, this means the binary fraction in the galactic nucleus is of the order few percent.Comment: 24 pages, 13 figures, submitted to Ap

SN 2020jgb: A Peculiar Type Ia Supernova Triggered by a Massive Helium-Shell Detonation in a Star-Forming Galaxy

The detonation of a thin ($\lesssim$$0.03\,\mathrm{M_\odot}$) helium shell (He-shell) atop a $\sim$$1\,\mathrm{M_\odot}$ white dwarf (WD) is a promising mechanism to explain normal Type Ia supernovae (SNe Ia), while thicker He-shells and less massive WDs may explain some recently observed peculiar SNe Ia. We present observations of SN 2020jgb, a peculiar SN Ia discovered by the Zwicky Transient Facility (ZTF). Near maximum light, SN 2020jgb is slightly subluminous (ZTF $g$-band absolute magnitude $M_g$ between $-18.2$ and $-18.7$ mag depending on the amount of host galaxy extinction) and shows an unusually red color ($g_\mathrm{ZTF}-r_\mathrm{ZTF}$ between 0.4 and 0.2 mag) due to strong line-blanketing blueward of $\sim$5000 $\r{A}$. These properties resemble those of SN 2018byg, a peculiar SN Ia consistent with a thick He-shell double detonation (DDet) SN. Using detailed radiative transfer models, we show that the optical spectroscopic and photometric evolution of SN 2020jgb are broadly consistent with a $\sim$$0.95\,\mathrm{M_\odot}$ (C/O core + He-shell; up to $\sim$$1.00\,\mathrm{M_\odot}$ depending on the total host extinction) progenitor ignited by a thick ($\sim$$0.13\,\mathrm{M_\odot}$) He-shell. We detect a prominent absorption feature at $\sim$1 $\mu\mathrm{m}$ in the near-infrared (NIR) spectrum of SN 2020jgb, which could originate from unburnt helium in the outermost ejecta. While the sample size is limited, similar 1 $\mu\mathrm{m}$ features have been detected in all the thick He-shell DDet candidates with NIR spectra obtained to date. SN 2020jgb is also the first subluminous, thick He-shell DDet SN discovered in a star-forming galaxy, indisputably showing that He-shell DDet objects occur in both star-forming and passive galaxies, consistent with the normal SN Ia population.Comment: 23 pages, 10 figures. Updated to accepted version (ApJ

SN 2020udy: a SN Iax with strict limits on interaction consistent with a helium-star companion

Early observations of transient explosions can provide vital clues to their progenitor origins. In this paper we present the nearby Type Iax (02cx-like) supernova (SN), SN 2020udy that was discovered within hours ($\sim$7 hr) of estimated first light. An extensive dataset of ultra-violet, optical, and near-infrared observations was obtained, covering out to $\sim$150 d after explosion. SN 2020udy peaked at -17.86$\pm$0.43 mag in the r band and evolved similarly to other 'luminous' SNe Iax, such as SNe 2005hk and 2012Z. Its well-sampled early light curve allows strict limits on companion interaction to be placed. Main-sequence companion stars with masses of 2 and 6 M$_\odot$ are ruled out at all viewing angles, while a helium-star companion is allowed from a narrow range of angles (140-180$^\circ$ away from the companion). The spectra and light curves of SN2020udy are in good agreement with those of the 'N5def' deflagration model of a near Chandrasekhar-mass carbon-oxygen white dwarf. However, as has been seen in previous studies of similar luminosity events, SN 2020udy evolves slower than the model. Broad-band linear polarisation measurements taken at and after peak are consistent with no polarisation, in agreement with the predictions of the companion-star configuration from the early light curve measurements. The host galaxy environment is low metallicity and is consistent with a young stellar population. Overall, we find the most plausible explosion scenario to be the incomplete disruption of a CO white dwarf near the Chandrasekhar-mass limit, with a helium-star companion.Comment: 18 pages, 14 figures, submitted to MNRA

Long-rising Type II Supernovae in the Zwicky Transient Facility Census of the Local Universe

SN 1987A was an unusual hydrogen-rich core-collapse supernova originating from a blue supergiant star. Similar blue supergiant explosions remain a small family of events, and are broadly characterized by their long rises to peak. The Zwicky Transient Facility (ZTF) Census of the Local Universe (CLU) experiment aims to construct a spectroscopically complete sample of transients occurring in galaxies from the CLU galaxy catalog. We identify 13 long-rising (>40 days) Type II supernovae from the volume-limited CLU experiment during a 3.5 year period from June 2018 to December 2021, approximately doubling the previously known number of these events. We present photometric and spectroscopic data of these 13 events, finding peak r-band absolute magnitudes ranging from -15.6 to -17.5 mag and the tentative detection of Ba II lines in 9 events. Using our CLU sample of events, we derive a long-rising Type II supernova rate of $1.37^{+0.26}_{-0.30}\times10^{-6}$ Mpc$^{-3}$ yr$^{-1}$, $\approx$1.4% of the total core-collapse supernova rate. This is the first volumetric rate of these events estimated from a large, systematic, volume-limited experiment.Comment: 32 pages, 17 figures, 5 tables. Submitted to Ap

The Broad-lined Ic Supernova ZTF18aaqjovh (SN 2018bvw): An Optically-discovered Engine-driven Supernova Candidate with Luminous Radio Emission

We present ZTF18aaqjovh (SN 2018bvw), a high-velocity ("broad-lined") stripped-envelope (Type Ic) supernova (Ic-BL SN) discovered in the Zwicky Transient Facility one-day cadence survey. ZTF18aaqjovh shares a number of features in common with engine-driven explosions: the photospheric velocity and the shape of the optical light curve are very similar to those of the Type Ic-BL SN 1998bw, which was associated with a low-luminosity gamma-ray burst (LLGRB) and had relativistic ejecta. However, the radio luminosity of ZTF18aaqjovh is almost two orders of magnitude fainter than that of SN 1998bw at the same velocity phase, and the shock velocity is at most mildly relativistic (v = 0.06–0.4c). A search of high-energy catalogs reveals no compelling gamma-ray burst (GRB) counterpart to ZTF18aaqjovh, and the limit on the prompt GRB luminosity of L_(γ,iso)≈1.6×10⁴⁸ erg/s⁻¹ excludes a classical GRB but not an LLGRB. Altogether, ZTF18aaqjovh represents another transition event between engine-driven SNe associated with GRBs and "ordinary" Ic-BL SNe

Tidal Disruption Event Demographics with the Zwicky Transient Facility: Volumetric Rates, Luminosity Function, and Implications for the Local Black Hole Mass Function

We conduct a systematic tidal disruption event (TDE) demographics analysis using the largest sample of optically selected TDEs. A flux-limited, spectroscopically complete sample of 33 TDEs is constructed using the Zwicky Transient Facility over three years (from October 2018 to September 2021). We infer the black hole (BH) mass ($M_{\rm BH}$) with host galaxy scaling relations, showing that the sample $M_{\rm BH}$ ranges from $10^{5.1}\,M_\odot$ to $10^{8.2}\,M_\odot$. We developed a survey efficiency corrected maximum volume method to infer the rates. The rest-frame $g$-band luminosity function (LF) can be well described by a broken power-law of $\phi (L_g)\propto [(L_g / L_{\rm bk})^{0.3} + (L_g / L_{\rm bk})^{2.6}]^{-1}$, with $L_{\rm bk}=10^{43.1}\,{\rm erg\,s^{-1}}$. In the BH mass regime of $10^{5.3}\lesssim (M_{\rm BH}/M_\odot) \lesssim 10^{7.3}$, the TDE mass function follows $\phi(M_{\rm BH})\propto M_{\rm BH}^{-0.25}$, which favors a flat local BH mass function ($dn_{\rm BH}/d{\rm log}M_{\rm BH}\approx{\rm constant}$). We confirm the significant rate suppression at the high-mass end ($M_{\rm BH}\gtrsim 10^{7.5}\,M_\odot$), which is consistent with theoretical predictions considering direct capture of hydrogen-burning stars by the event horizon. At a host galaxy mass of $M_{\rm gal}\sim 10^{10}\,M_\odot$, the average optical TDE rate is $\approx 3.2\times 10^{-5}\,{\rm galaxy^{-1}\,yr^{-1}}$. We constrain the optical TDE rate to be [3.7, 7.4, and 1.6$]\times 10^{-5}\,{\rm galaxy^{-1}\,yr^{-1}}$ in galaxies with red, green, and blue colors.Comment: Replaced following peer-review process. 38 pages, 23 figures. Accepted for publication in ApJ