Damped kink motions in a system of two solar coronal tubes with elliptic cross-sections

This study is motivated by observations of coordinated transverse displacements in neighboring solar active region loops, addressing specifically how the behavior of kink motions in straight two-tube equilibria is impacted by tube interactions and tube cross-sectional shapes.We work with linear, ideal, pressureless magnetohydrodynamics. Axially standing kink motions are examined as an initial value problem for transversely structured equilibria involving two identical, field-aligned, density-enhanced tubes with elliptic cross-sections (elliptic tubes). Continuously nonuniform layers are implemented around both tube boundaries. We numerically follow the system response to external velocity drivers, largely focusing on the quasi-mode stage of internal flows to derive the pertinent periods and damping times. The periods and damping times we derive for two-circular-tube setups justify available modal results found with the T-matrix approach. Regardless of cross-sectional shapes, our nonuniform layers feature the development of small-scale shears and energy accumulation around Alf\'ven resonances, indicative of resonant absorption and phase-mixing. As with two-circular-tube systems, our configurational symmetries make it still possible to classify lower-order kink motions by the polarization and symmetric properties of the internal flows; hence such mode labels as $S_x$ and $A_x$. However, the periods and damping times for two-elliptic-tube setups further depend on cross-sectional aspect ratios, with $A_x$ motions occasionally damped less rapidly than $S_x$ motions. We find uncertainties up to $\sim 20\%$ ($\sim 50\%$) for the axial Alfven time (the inhomogeneity lengthscale) if the periods (damping times) computed for two-elliptic-tube setups are seismologically inverted with canonical theories for isolated circular tubes.Comment: Accepted for publication in A&

A novel intelligent adaptive control of laser-based ground thermal test

AbstractLaser heating technology is a type of potential and attractive space heat flux simulation technology, which is characterized by high heating rate, controlled spatial intensity distribution and rapid response. However, the controlled plant is nonlinear, time-varying and uncertainty when implementing the laser-based heat flux simulation. In this paper, a novel intelligent adaptive controller based on proportion–integration–differentiation (PID) type fuzzy logic is proposed to improve the performance of laser-based ground thermal test. The temperature range of thermal cycles is more than 200K in many instances. In order to improve the adaptability of controller, output scaling factors are real time adjusted while the thermal test is underway. The initial values of scaling factors are optimized using a stochastic hybrid particle swarm optimization (H-PSO) algorithm. A validating system has been established in the laboratory. The performance of the proposed controller is evaluated through extensive experiments under different operating conditions (reference and load disturbance). The results show that the proposed adaptive controller performs remarkably better compared to the conventional PID (PID) controller and the conventional PID type fuzzy (F-PID) controller considering performance indicators of overshoot, settling time and steady state error for laser-based ground thermal test. It is a reliable tool for effective temperature control of laser-based ground thermal test

Architectural principles for Hfq/Crc-mediated regulation of gene expression.

In diverse bacterial species, the global regulator Hfq contributes to post-transcriptional networks that control expression of numerous genes. Hfq of the opportunistic pathogen Pseudomonas aeruginosa inhibits translation of target transcripts by forming a regulatory complex with the catabolite repression protein Crc. This repressive complex acts as part of an intricate mechanism of preferred nutrient utilisation. We describe high-resolution cryo-EM structures of the assembly of Hfq and Crc bound to the translation initiation site of a target mRNA. The core of the assembly is formed through interactions of two cognate RNAs, two Hfq hexamers and a Crc pair. Additional Crc protomers are recruited to the core to generate higher-order assemblies with demonstrated regulatory activity in vivo. This study reveals how Hfq cooperates with a partner protein to regulate translation, and provides a structural basis for an RNA code that guides global regulators to interact cooperatively and regulate different RNA targets.BFL, XYP and TD are supported by the Welcome Trust (200873/Z/16/Z). TD is also supported by an AstraZeneca Studentship. UB and ES are supported by the Austrian Science Fund (FWF) (www.fwf.ac.at/en) [P28711-B22]

Establishment of an efficient plant regeneration culture protocol and achievement of successful genetic transformation in Jatropha curcas L.

An efficient and reproducible protocol is described for shoot-bud regeneration and Agrobacterium tumefaciens-mediated genetic transformation of J. curcas. Treating the explants with high concentrations (5–120 mg/L) of TDZ for short durations (5–80 min) before inoculation culture increased significantly the regeneration frequency and improved the quality of the regenerated buds. The highest shoot-buds induction rate (87.35%) was achieved when petiole explants were treated with 20 mg/L TDZ solution for 20 min and inoculated on hormone-free MS medium for 30 days. Regenerated shoots of 0.5 cm or a little longer were isolated and grafted to seedling stocks of the same species, and then the grafted plantlets were planted on half-strength MS medium containing 0.1 mg/L IBA and 2 mg/L sodium nitroprusside (SNP). This grafting strategy was found to be very effective, to obtain that healthy grafted plantlets ready for acclimatization within 20 days. By the above mentioned protocol and with general Agrobacterium – mediated genetic transformation methods only 65 days were needed to obtain intact transgenic plants

High-resolution structure of the presynaptic RAD51 filament on single-stranded DNA by electron cryo-microscopy

Homologous DNA recombination (HR) by the RAD51 recombinase enables error-free DNA break repair. To execute HR, RAD51 first forms a presynaptic filament on single-stranded (ss) DNA, which catalyses pairing with homologous double-stranded (ds) DNA. Here, we report a structure for the presynaptic human RAD51 filament at 3.5-5.0Å resolution using electron cryo-microscopy. RAD51 encases ssDNA in a helical filament of 103Å pitch, comprising 6.4 protomers per turn, with a rise of 16.1Å and a twist of 56.2°. Inter-protomer distance correlates with rotation of an α-helical region in the core catalytic domain that is juxtaposed to ssDNA, suggesting how the RAD51-DNA interaction modulates protomer spacing and filament pitch. We map Fanconi anaemia-like disease-associated RAD51 mutations, clarifying potential phenotypes. We predict binding sites on the presynaptic filament for two modules present in each BRC repeat of the BRCA2 tumour suppressor, a critical HR mediator. Structural modelling suggests that changes in filament pitch mask or expose one binding site with filament-inhibitory potential, rationalizing the paradoxical ability of the BRC repeats to either stabilize or inhibit filament formation at different steps during HR. Collectively, our findings provide fresh insight into the structural mechanism of HR and its dysregulation in human diseaseThis is the final version of the article. It first appeared from Oxford University Press via http://dx.doi.org/10.1093/nar/gkw78

Neuroprotective Effect of Xueshuantong for Injection (Lyophilized) in Transient and Permanent Rat Cerebral Ischemia Model

Xueshuantong for Injection (Lyophilized) (XST), a Chinese Materia Medica standardized product extracted from Panax notoginseng (Burk.), is used extensively for the treatment of cerebrovascular diseases such as acutely cerebral infarction clinically in China. In the present study, we evaluated the acute and extended protective effects of XST in different rat cerebral ischemic model and explored its effect on peroxiredoxin (Prx) 6-toll-like receptor (TLR) 4 signaling pathway. We found that XST treatment for 3 days could significantly inhibit transient middle cerebral artery occlusion (MCAO) induced infarct volume and swelling percent and regulate the mRNA expression of interleukin-1β (IL-1β), IL-17, IL-23p19, tumor necrosis factor-α (TNFα), and inducible nitric oxide synthase (iNOS) in brain. Further study demonstrated that treatment with XST suppressed the protein expression of peroxiredoxin (Prx) 6-toll-like receptor (TLR) 4 and phosphorylation level of p38 and upregulated the phosphorylation level of STAT3. In permanent MCAO rats, XST could reduce the infarct volume and swelling percent. Moreover, our results revealed that XST treatment could increase the rats’ weight and improve a batch of functional outcomes. In conclusion, the present data suggested that XST could protect against ischemia injury in transient and permanent MCAO rats, which might be related to Prx6-TLR4 pathway

Investigation of thermal dynamics for different leading configuration in hybrid laser-MIG welding

For hybrid laser-MIG welding of aluminum alloy, the weld bead quality is decided by the thermal dynamics in the molten pool. In order to analyze the influence of leading configuration on thermal behavior and mass transfer, a three-dimensional numerical model is established. In the model, a combined model is utilized to simulate the multiple reflections and Fresnel absorption of laser beam. The laser-arc interaction is considered and the plasma is shrinked for arc-leading configuration. Surface tension, electromagnetic force, buoyancy are considered to investigate the fluid flow pattern, and droplet impact and arc pressure are taken into account to track the free surface. For different configurations, the temperature and velocity field, together with concentration distribution of magnesium and zinc are compared and analyzed. The results show that transport phenomena of the molten pool is strongly affected by the configuration. For arc-leading (AL) configuration, it is easier to get a larger penetration. Meanwhile, the concentration distribution of magnesium and zinc is more homogeneous for laser-leading (LL) configuration. Furthermore, the developed model can provide theoretical basis for processing experiments