Efecto de extractos crudos y aceites esenciales de plantas aromáticas contra Sclerotinia sclerotiorum en campos de papa

This study was carried out to determine inhibitory effect of crude extracts and essential oils (OE) of aromatic plants against Sclerotinia sclerotiorum in potato fields in Bahar and Lalehjin, Iran during cropping seasons of 2015 and 2016. The results showed that crude extracts of cinnamon and ginger at concentrations 5 g/l as well as essential oils of coriander, lavender, thyme, savory and eucalyptus at concentration 50 µl/ml had potential to significantly reduce incidence of potato white mold. This is the first study about inhibitory effect of crude extracts and essential oils of aromatic plants against potato white mold under field conditionEste estudio se realizó para determinar el efecto inhibitorio de los extractos crudos y aceites esenciales de plantas aromáticas (AE) contra Sclerotinia sclerotiorum en los campos de papa en Bahar y Lalehjin, Irán, durante las temporadas de cultivo de 2015 y 2016. Los resultados mostraron que los extractos crudos de canela y jengibre en concentraciones 5 g/l, así como los aceites esenciales de cilantro, tomillo, lavanda, ajedrea y eucalipto a una concentración de 50 μl/ml tenían el potencial de reducir significativamente la incidencia de moho blanco de papa. Este es el primer estudio sobre el efecto inhibitorio de los extractos crudos y aceites esenciales de plantas aromáticas contra el moho blanco de la papa en condiciones de campo

Forecasting of potato white mold by assessment of ascospores in Iran fields

Potato white mold caused by Sclerotinia sclerotiorum is an important plant disease occurring in many potato-producing areas throughout the world. In this study, a specific diagnostic method was used to detect and quantify S. sclerotiorum ascospores, and its forecasting ability was assessed in potato fields during flowering periods of 2011 to 2014 in Bahar County, Hamedan Province. Using GenEMBL database, a primer pair, HZSCREV and HZSCFOR, was designed and optimized for the pathogen. After testing the sensitivity of primers, DNA was extracted from samples of outdoor Burkard traps from potato fields. A linear association was observed between pathogen DNA and the number of ascospores using the quantitative PCR (qPCR) technique in the presence of SYBR dye. The qPCR could successfully detect DNA amounts representing two S. sclerotiorum ascospores and was not sensitive to a variety of tested fungi such as Botrytis cinerea, Alternaria brassicae, Fusarium solani. In contrast to the amount of rainfall, a direct relationship was found between ascospore numbers and the incidence of potato white mold from 2011 to 2014