15 research outputs found
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Inhibition of adenosine 3′,5′-monophosphate accumulation in anterior pituitary gland in vitro by growth hormone-release inhibiting hormone
1 × 10
−7M growth hormone-release inhibiting hormone (GH-RIH or somatostatin) leads to a 50% inhibition of adenosine 3′,5′-monophosphate (cyclic AMP) accumulation in anterior pituitary tissue during the first 2 min of incubation. This lowering of cyclic AMP levels is accompanied by inhibition of the release of immunoreactive growth hormone and thyrotropin. GH-RIH has also a marked inhibitory effect on the theophyline- and prostaglandin E
2-induced accumulation of adenohypophyseal cyclic AMP
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Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone
Addition of increasing doses of synthetic growth hormone-release inhibiting hormone (GH-RIH) leads to a progressive decrease of the basal and N
6monobutyryl cyclic AMP-,theophylline- and prostaglandin E
2-induced release of immunoreactive growth hormone (GH) and thyrotropin (TSH) release from rat anterior pituitary cells in monolayer culture. A halfmaximal effect is measured at 3 × 10
−9 M GH-RIH while a maximal inhibition to 10–20% of the control level is found at 1 × 10
−7 M. Using rat hemipituitaries and measurement of GH release by both polyacrylamide gel electrophoresis and radioimmunoassay, a maximal effect of GH-RIH was found in the first 5 min of incubation. The inhibitory effect of GH-RIH on GH release remained constant for at least 3 h. GH-RIH does not affect the basal or induced release of prolactin and luteinizing hormone nor the high K
+-induced release of GH and TSH