Mechanobiology of Antimicrobial Resistant Escherichia coli and Listeria innocua

A majority of antibiotic-resistant bacterial infections in the United States are associated with biofilms. Nanoscale biophysical measures are increasingly revealing that adhesive and viscoelastic properties of bacteria play essential roles across multiple stages of biofilm development. Atomic Force Microscopy (AFM) applied to strains with variation in antimicrobial resistance enables new opportunities for investigating the function of adhesive forces (stickiness) in biofilm formation. AFM force spectroscopy analysis of a field strain of Listeria innocua and the strain Escherichia coli K-12 MG1655 revealed differing adhesive forces between antimicrobial resistant and nonresistant strains. Significant increases in stickiness were found at the nanonewton level for strains of Listeria innocua and Escherichia coli in association with benzalkonium chloride and silver nanoparticle resistance respectively. This advancement in the usage of AFM provides for a fast and reliable avenue for analyzing antimicrobial resistant cells and the molecular dynamics of biofilm formation as a protective mechanism

Studying Group Decision Making in Affinity Diagramming

Affinity diagramming is a commonly used contextual design practice for which many tools have been developed. However, experts and novices alike eschew tool use, instead using traditional paper and whiteboard methods. This paper presents observations of traditional affinity diagramming sessions, focusing on three areas of consideration—shared awareness, cognitive offloading, and understanding, organizing and searching—that are important for collaborative tools. Specific design requirements for each of these three areas are described

USMC VERTICAL TAKEOFF AND LANDING AIRCRAFT: HUMAN–MACHINE TEAMING FOR CONTROLLING UNMANNED AERIAL SYSTEMS

The United States Marine Corps (USMC) is investing in aviation technologies through its Vertical Takeoff and Landing (VTOL) aircraft program that will enhance mission superiority and warfare dominance against both conventional and asymmetric threats. One of the USMC program initiatives is to launch unmanned aerial systems (UAS) from future human-piloted VTOL aircraft for collaborative hybrid (manned and unmanned) missions. This hybrid VTOL-UAS capability will support USMC intelligence, surveillance, and reconnaissance (ISR), electronic warfare (EW), communications relay, and kinetic strike air to ground missions. This capstone project studied the complex human-machine interactions involved in the future hybrid VTOL-UAS capability through model-based systems engineering analysis, coactive design interdependence analysis, and modeling and simulation experimentation. The capstone focused on a strike coordination and reconnaissance (SCAR) mission involving a manned VTOL platform, a VTOL-launched UAS, and a ground control station (GCS). The project produced system requirements, a system architecture, a conceptual design, and insights into the human-machine teaming aspects of this future VTOL capability.Major, United States ArmyMajor, United States ArmyMajor, United States ArmyMajor, United States ArmyMajor, United States ArmyApproved for public release. Distribution is unlimited

Model-based reasoning: using visual tools to reveal student learning

Luckie D, Harrison SH, Ebert-May D. Model-based reasoning: using visual tools to reveal student learning

Dip coating process: Silicon sheet growth development for the large-area silicon sheet task of the low-cost silicon solar array project

The technical and economic feasibility of producing solar cell quality sheet silicon by dip-coating one surface of carbonized ceramic substrates with a thin layer of large grain polycrystalline silicon was investigated. The dip-coating methods studied were directed toward a minimum cost process with the ultimate objective of producing solar cells with a conversion efficiency of 10% or greater. The technique shows excellent promise for low cost, labor-saving, scale-up potentialities and would provide an end product of sheet silicon with a rigid and strong supportive backing. An experimental dip-coating facility was designed and constructed, several substrates were successfully dip-coated with areas as large as 25 sq cm and thicknesses of 12 micron to 250 micron. There appears to be no serious limitation on the area of a substrate that could be coated. Of the various substrate materials dip-coated, mullite appears to best satisfy the requirement of the program. An inexpensive process was developed for producing mullite in the desired geometry

A Candidate Dual Active Galactic Nucleus At Z=1.175

The X-ray source CXOXBJ142607.6+353351 (CXOJ1426+35), which was identified in a 172 ks Chandra image in the Bootes field, shows double-peaked rest-frame optical/UV emission lines, separated by 0.''69 (5.5 kpc) in the spatial dimension and by 690 km s(-1) in the velocity dimension. The high excitation lines and emission line ratios indicate both systems are ionized by an active galactic nucleus (AGN) continuum, and the double-peaked profile resembles that of candidate dual AGNs. At a redshift of z = 1.175, this source is the highest redshift candidate dual AGN yet identified. However, many sources have similar emission line profiles for which other interpretations are favored. We have analyzed the substantial archival data available in this field as well as acquired near-infrared (NIR) adaptive optics (AO) imaging and NIR slit spectroscopy. The X-ray spectrum is hard, implying a column density of several 10(23) cm(-2). Though heavily obscured, the source is also one of the brightest in the field, with an absorption-corrected 2-10 keV luminosity of similar to 10(45) erg s(-1). Outflows driven by an accretion disk may produce the double-peaked lines if the central engine accretes near the Eddington limit. However, we may be seeing the narrow line regions of two AGNs following a galactic merger. While the AO image reveals only a single source, a second AGN would easily be obscured by the significant extinction inferred from the X-ray data. Understanding the physical processes producing the complex emission line profiles seen in CXOJ1426+35 and related sources is important for interpreting the growing population of dual AGN candidates.National Science Foundation AST-0708490Strategic University Research Partnership ProgramNational Aeronautics and Space AdministrationW. M. Keck FoundationSmithsonian Astrophysical Observatory SV4-74018, A31Astronom