Mrub_1873, Mrub_1872, Mrub_1871 genes are predicted orthologs of the b2285, b2284, and b2283 genes respectively, found in \u3cem\u3eEscherichia coli\u3c/em\u3e coding for NADH ubiquinone oxidoreductase complex subunits E, F, and G.

This project is part of the Meiothermus ruber genome analysis project, which uses the bioinformatics tools associated with the Guiding Education through Novel Investigation –Annotation Collaboration Toolkit (GENI-ACT) to predict gene function. We investigated the biological function of the genes Mrub_1873, Mrub_1872, and Mrub_1871.We predict that Mrub_1873 (DNA coordinates 1933743..1934309 on the reverse strand), Mrub_1872 (DNA coordinates 1932430..1933746 on the reverse strand), and Mrub_1871 (DNA coordinates 1930055..1932421 on the reverse strand) are subunits of the NADH ubiquinone oxidoreductase complex (00190). The complex catalyzes both the transfer of protons across the cytoplasmic membrane and the transfer of electrons to ubiquinone during the process of oxidative phosphorylation. The E. coli K12 MG1655 orthologs are predicted to be b2285, b2284, and b2283, which have the gene identifiers NuoE, NuoF, NuoG respectively

Strategic learning and teaching enhancement through funded teaching interventions

A case study of the Learning and Teaching Development Fund at University of Glasgow. In order to maintain the pace of learning and teaching development in line with recent changes in the student population (such as demographics, numbers, technological skill level and expectations) it is important for institutions such as Glasgow to support excellent and innovative learning and teaching at strategic levels. Strategic initiatives must be meaningful to encourage the appropriate changes in learning and teaching practices. The University of Glasgow has, since 2000-01, implemented several initiatives to specifically enhance learning and teaching. One of the longest-standing of these is the Learning and Teaching Development Fund (the LTDF). Annual bids are invited from members of staff for projects that aim to enhance learning and teaching and that align with key strategic aims

Optimization of over-summer snow storage at midlatitudes and low elevation

Climate change, including warmer winter temperatures, a shortened snowfall season, and more rain-on-snow events, threatens nordic skiing as a sport. In response, oversummer snow storage, attempted primarily using woodchips as a cover material, has been successfully employed as a climate change adaptation strategy by high-elevation and/or high-latitude ski centers in Europe and Canada. Such storage has never been attempted at a site that is both low elevation and midlatitude, and few studies have quantified storage losses repeatedly through the summer. Such data, along with tests of different cover strategies, are prerequisites to optimizing snow storage strategies. Here, we assess the rate at which the volume of two woodchip-covered snow piles (each ∼ 200 m3), emplaced during spring 2018 in Craftsbury, Vermont (45° N and 360 m a.s.l.), changed. We used these data to develop an optimized snow storage strategy. In 2019, we tested that strategy on a much larger, 9300 m3 pile. In 2018, we continually logged air-to-snow temperature gradients under different cover layers including rigid foam, open-cell foam, and woodchips both with and without an underlying insulating blanket and an overlying reflective cover. We also measured ground temperatures to a meter depth adjacent to the snow piles and used a snow tube to measure snow density. During both years, we monitored volume change over the melt season using terrestrial laser scanning every 10- 14 d from spring to fall. In 2018, snow volume loss ranged from 0.29 to 2.81 m3 d-1, with the highest rates in midsummer and lowest rates in the fall; mean rates of volumetric change were 1.24 and 1.50 m3 d-1, 0.55 % to 0.72 % of initial pile volume per day. Snow density did increase over time, but most volume loss was the result of melting. Wet woodchips underlain by an insulating blanket and covered with a reflective sheet were the most effective cover combination for minimizing melt, likely because the aluminized surface reflected incoming short-wave radiation while the wet woodchips provided significant thermal mass, allowing much of the energy absorbed during the day to be lost by long-wave emission at night. The importance of the pile surface-area-tovolume ratio is demonstrated by 4-fold lower rates of volumetric change for the 9300 m3 pile emplaced in 2019; it lost \u3c 0:16 % of its initial volume per day between April and October, retaining ∼ 60 % of the initial snow volume over summer. Together, these data demonstrate the feasibility of oversummer snow storage at midlatitudes and low elevations and suggest efficient cover strategies

Contrasting roles for DNA methyltransferases and histone deacetylases in single-item and associative recognition memory

Recognition memory enables us to judge whether we have encountered a stimulus before and to recall associated information, including where the stimulus was encountered. The perirhinal cortex (PRh) is required for judgment of stimulus familiarity, while hippocampus (HPC) and medial prefrontal cortex (mPFC) are additionally involved when spatial information associated with a stimulus needs to be remembered. While gene expression is known to be essential for the consolidation of long-term recognition memory, the underlying regulatory mechanisms are not fully understood. Here we investigated the roles of two epigenetic mechanisms, DNA methylation and histone deacetylation, in recognition memory. Infusion of DNA methyltransferase inhibitors into PRh impaired performance in novel object recognition and object-in-place tasks while infusions into HPC or mPFC impaired object-in-place performance only. In contrast, inhibition of histone deacetylases in PRh, but not mPFC, enhanced recognition memory. These results support the emerging role of epigenetic processes in learning and memory

Early childhood lung function is a stronger predictor of adolescent lung function in cystic fibrosis than early Pseudomonas aeruginosa infection

Pseudomonas aeruginosa has been suggested as a major determinant of poor pulmonary outcomes in cystic fibrosis (CF), although other factors play a role. Our objective was to investigate the association of early childhood Pseudomonas infection on differences in lung function in adolescence with CF

Preeclamptic placentae release factors that damage neurons: implications for foetal programming of disease

Prenatal development is a critical period for programming of neurological disease. Preeclampsia, a pregnancy complication involving oxidative stress in the placenta, has been associated with long-term health implications for the child, including an increased risk of developing schizophrenia and autism spectrum disorders in later life. To investigate if molecules released by the placenta may be important mediators in foetal programming of the brain, we analysed if placental tissue delivered from patients with preeclampsia secreted molecules that could affect cortical cells in culture. Application of culture medium conditioned by preeclamptic placentae to mixed cortical cultures caused changes in neurons and astrocytes that were related to key changes observed in brains of patients with schizophrenia and autism, including effects on dendrite lengths, astrocyte number as well as on levels of glutamate and γ-aminobutyric acid receptors. Treatment of the placental explants with an antioxidant prevented neuronal abnormalities. Furthermore, we identified that bidirectional communication between neurons and astrocytes, potentially via glutamate, is required to produce the effects of preeclamptic placenta medium on cortical cells. Analysis of possible signalling molecules in the placenta-conditioned medium showed that the secretion profile of extracellular microRNAs, small post-transcriptional regulators, was altered in preeclampsia and partially rescued by antioxidant treatment of the placental explants. Predicted targets of these differentially abundant microRNAs were linked to neurodevelopment and the placenta. The present study provides further evidence that the diseased placenta may release factors that damage cortical cells and suggests the possibility of targeted antioxidant treatment of the placenta to prevent neurodevelopmental disorders

Bait flavor preference and immunogenicity of ONRAB baits in domestic dogs on the Navajo Nation, Arizona

Rabies is responsible for an estimated 59,000 human deaths worldwide, and domestic dogs are the primary reservoir and vector of the disease. Among some nations, widespread vaccination has led to elimination of rabies in domestic dogs, yet dogs are still susceptible to rabies infection from interactions with wildlife reservoirs. On Tribal lands in the United States, less than 20% of domestic dogs are vaccinated for rabies, and parenteral vaccination is often unfeasible. Oral rabies vaccination may provide a solution, but a suitable bait flavor and vaccine must be identified. We evaluated 5 bait flavors (bacon, cheese, egg, fish, and sweet) in pairwise flavor-preference trials using placebo Ultralite baits in 26 domestic dogs on the Navajo Nation, Arizona. Each bait flavor was offered a total of 104 times. In all paired comparisons, bacon was more frequently preferred to the alternative. The sweet flavor (the flavor used operationally for oral rabies vaccine (ORV) distribution in Canada) was least preferred. Forty domestic dogs were offered baits containing ONRAB ORV: 14 received the sweet-flavored bait packet and 26 received bacon-flavored baits. Serum was collected from dogs before vaccination and at day 14 and 30 or 37 days after vaccination. Thirty-seven dogs consumed the baits, 2 baits (both sweet flavored) were chewed and spit out, and 1 (sweet flavored) was swallowed without apparent chewing (gulped). Eight dogs had preexisting rabies virus neutralizing antibody (RVNA) titers and 13 naïve dogs failed to seroconvert during the study period. Overall, 27 dogs (67.5%) showed increased RVNA titers after vaccination, including 1 dog who chewed and spit out the bait and all dogs with positive baseline RVNA titers. Geometric mean titers for all dogs that seroconverted during the study period peaked at day 14 (1.2 IU/ mL; n = 24) and decreased slightly by the final sampling day (0.8 IU/mL; n = 27).We conclude that bacon flavor may be a suitable bait flavor for ORV distribution in loosely kept or free-roaming domestic dogs. Seroconversion among dogs who ingested ONRAB-filled baits was variable. Why 13 dogs who consumed ORV baits failed to seroconvert remains unknown. Additional research to improve seroconversion rates in domestic dogs after vaccination with ONRAB is recommended

Bait flavor preference and immunogenicity of ONRAB baits in domestic dogs on the Navajo Nation, Arizona

Rabies is responsible for an estimated 59,000 human deaths worldwide, and domestic dogs are the primary reservoir and vector of the disease. Among some nations, widespread vaccination has led to elimination of rabies in domestic dogs, yet dogs are still susceptible to rabies infection from interactions with wildlife reservoirs. On Tribal lands in the United States, less than 20% of domestic dogs are vaccinated for rabies, and parenteral vaccination is often unfeasible. Oral rabies vaccination may provide a solution, but a suitable bait flavor and vaccine must be identified. We evaluated 5 bait flavors (bacon, cheese, egg, fish, and sweet) in pairwise flavor-preference trials using placebo Ultralite baits in 26 domestic dogs on the Navajo Nation, Arizona. Each bait flavor was offered a total of 104 times. In all paired comparisons, bacon was more frequently preferred to the alternative. The sweet flavor (the flavor used operationally for oral rabies vaccine (ORV) distribution in Canada) was least preferred. Forty domestic dogs were offered baits containing ONRAB ORV: 14 received the sweet-flavored bait packet and 26 received bacon-flavored baits. Serum was collected from dogs before vaccination and at day 14 and 30 or 37 days after vaccination. Thirty-seven dogs consumed the baits, 2 baits (both sweet flavored) were chewed and spit out, and 1 (sweet flavored) was swallowed without apparent chewing (gulped). Eight dogs had preexisting rabies virus neutralizing antibody (RVNA) titers and 13 naïve dogs failed to seroconvert during the study period. Overall, 27 dogs (67.5%) showed increased RVNA titers after vaccination, including 1 dog who chewed and spit out the bait and all dogs with positive baseline RVNA titers. Geometric mean titers for all dogs that seroconverted during the study period peaked at day 14 (1.2 IU/ mL; n = 24) and decreased slightly by the final sampling day (0.8 IU/mL; n = 27).We conclude that bacon flavor may be a suitable bait flavor for ORV distribution in loosely kept or free-roaming domestic dogs. Seroconversion among dogs who ingested ONRAB-filled baits was variable. Why 13 dogs who consumed ORV baits failed to seroconvert remains unknown. Additional research to improve seroconversion rates in domestic dogs after vaccination with ONRAB is recommended

Changes in Energy Expenditure, Dietary Intake, and Energy Availability Across an Entire Collegiate Women’s Basketball Season: Erratum

Erratu

Evolution of Network Enumeration Strategies in Emulated Computer Networks

Successful attacks on computer networks today do not often owe their victory to directly overcoming strong security measures set up by the defender. Rather, most attacks succeed because the number of possible vulnerabilities are too large for humans to fully protect without making a mistake. Regardless of the security elsewhere, a skilled attacker can exploit a single vulnerability in a defensive system and negate the benefits of those security measures. This paper presents an evolutionary framework for evolving attacker agents in a real, emulated network environment using genetic programming, as a foundation for coevolutionary systems which can automatically discover and mitigate network security flaws. We examine network enumeration, an initial network reconnaissance step, through our framework and present results demonstrating its success, indicating a broader applicability to further cyber-security tasks