830 research outputs found
Land contributions to natural CO2 variability on time scales of centuries
The present paper addresses the origin of natural variability arising internally from the climate system of the global carbon cycle at centennial time scales. The investigation is based on the Max Planck Institute for Meteorology, Coupled Model Intercomparison Project Phase 5 (MPI-MCMIP5) preindustrial control simulations with the MPI Earth System Model in low resolution (MPI-ESM-LR) supplemented by additional simulations conducted for further analysis. The simulations show a distinct low-frequency component in the global terrestrial carbon content that induces atmospheric CO2 variations on centennial time scales of up to 3 ppm. The main drivers for these variations are low-frequency fluctuations in net primary production (NPP) of the land biosphere. The signal arises from small regions scattered across the whole globe with a pronounced source in North America. The main reason for the global NPP fluctuations is found in climatic changes leading to long-term variations in leaf area index, which largely determines the strength of photosynthetic carbon assimilation. The underlying climatic changes encompass several spatial diverse climatic alterations. For the particular case of North America, the carbon storage changes are (besides NPP) also dependent on soil respiration. This second mechanism is strongly connected to low-frequency variations in incoming shortwave radiation at the surface. Š2013. American Geophysical Union. All Rights Reserved
Understanding the âBerg limitâ: the 65° contact angle as the universal adhesion threshold of biomatter
Surface phenomena in aqueous environments such as long-range hydrophobic attraction, macromolecular adhesion, and even biofouling are predominantly influenced by a fundamental parameterâthe water contact angle. The minimal contact angle required for these and related phenomena to occur has been repeatedly reported to be around 65° and is commonly referred to as the âBerg limit.â However, the universality of this specific threshold across diverse contexts has remained puzzling. In this perspective article, we aim to rationalize the reoccurrence of this enigmatic contact angle. We show that the relevant scenarios can be effectively conceptualized as three-phase problems involving the surface of interest, water, and a generic oil-like material that is representative of the nonpolar constituents within interacting entities. Our analysis reveals that attraction and adhesion emerge when substrates display an underwater oleophilic character, corresponding to a âhydrophobicity under oilâ, which occurs for contact angles above approximately 65°. This streamlined view provides valuable insights into macromolecular interactions and holds implications for technological applications
Influence of polar co-solutes and salt on the hydration of lipid membranes
The influence of the co-solutes TMAO, urea, and NaCl on the hydration repulsion between lipid membranes is investigated in a combined experimental/simulation approach. Pressureâhydration curves obtained via sorption experiments reveal that the repulsion significantly increases when the membranes are loaded with co-solutes, most strongly for TMAO. As a result, the co-solutes retain additional water molecules and therefore provide membranes with a fluid and more physiological environment. The experimental data are quantitatively reproduced in complementary solvent-explicit atomistic molecular dynamics simulations, which yield the chemical potential of water. Simulation analysis reveals that the additional repulsion arises from the osmotic pressure generated by the co-solutes, an effect which is maximal for TMAO, due to its unfavorable interactions with the lipid headgroup layer and its extraordinarily high osmotic coefficient
Anti-CD20 therapy depletes activated myelin-specific CD8+ T cells in multiple sclerosis.
CD8+ T cells are believed to play an important role in multiple sclerosis (MS), yet their role in MS pathogenesis remains poorly defined. Although myelin proteins are considered potential autoantigenic targets, prior studies of myelin-reactive CD8+ T cells in MS have relied on in vitro stimulation, thereby limiting accurate measurement of their ex vivo precursor frequencies and phenotypes. Peptide:MHC I tetramers were used to identify and validate 5 myelin CD8+ T cell epitopes, including 2 newly described determinants in humans. The validated tetramers were used to measure the ex vivo precursor frequencies and phenotypes of myelin-specific CD8+ T cells in the peripheral blood of untreated MS patients and HLA allele-matched healthy controls. In parallel, CD8+ T cell responses against immunodominant influenza epitopes were also measured. There were no differences in ex vivo frequencies of tetramer-positive myelin-specific CD8+ T cells between MS patients and control subjects. An increased proportion of myelin-specific CD8+ T cells in MS patients exhibited a memory phenotype and expressed CD20 compared to control subjects, while there were no phenotypic differences observed among influenza-specific CD8+ T cells. Longitudinal assessments were also measured in a subset of MS patients subsequently treated with anti-CD20 monoclonal antibody therapy. The proportion of memory and CD20+ CD8+ T cells specific for certain myelin but not influenza epitopes was significantly reduced following anti-CD20 treatment. This study, representing a characterization of unmanipulated myelin-reactive CD8+ T cells in MS, indicates these cells may be attractive targets in MS therapy
Weak carbohydrate-carbohydrate interactions in membrane adhesion are fuzzy and generic
Carbohydrates such as the trisaccharide motif LeX are key constituents of
cell surfaces. Despite intense research, the interactions between carbohydrates
of apposing cells or membranes are not well understood. In this article, we
investigate carbohydrate-carbohydrate interactions in membrane adhesion as well
as in solution with extensive atomistic molecular dynamics simulations that
exceed the simulation times of previous studies by orders of magnitude. For
LeX, we obtain association constants of soluble carbohydrates, adhesion
energies of lipid-anchored carbohydrates, and maximally sustained forces of
carbohydrate complexes in membrane adhesion that are in good agreement with
experimental results in the literature. Our simulations thus appear to provide
a realistic, detailed picture of LeX-LeX interactions in solution and during
membrane adhesion. In this picture, the LeX-LeX interactions are fuzzy, i.e.
LeX pairs interact in a large variety of short-lived, bound conformations. For
the synthetic tetrasaccharide Lac 2, which is composed of two lactose units, we
observe similarly fuzzy interactions and obtain association constants of both
soluble and lipid-anchored variants that are comparable to the corresponding
association constants of LeX. The fuzzy, weak carbohydrate-carbohydrate
interactions quantified in our simulations thus appear to be a generic feature
of small, neutral carbohydrates such as LeX and Lac 2.Comment: 12 pages, 9 figure
Assessment of JSBACHv4.30 as a land component of ICON-ESM-V1 in comparison to its predecessor JSBACHv3.2 of MPI-ESM1.2
We assess the land surface model JSBACHv4 (Jena Scheme for Biosphere Atmosphere Coupling in Hamburg version 4), which was recently developed at the Max Planck Institute for Meteorology as part of the effort to build the new Icosahedral Nonhydrostatic (ICON) Earth system model (ESM), ICON-ESM. We assess JSBACHv4 in simulations coupled with ICON-A, the atmosphere model of ICON-ESM, hosting JSBACHv4 as land component to provide the surface boundary conditions. The assessment is based on a comparison of simulated albedo, land surface temperature (LST), leaf area index (LAI), terrestrial water storage (TWS), fraction of absorbed photosynthetic active radiation (FAPAR), net primary production (NPP), and water use efficiency (WUE) with corresponding observational data. JSBACHv4 is the successor of JSBACHv3; therefore, another purpose of this study is to document how this step in model development has changed model biases. This is achieved by also assessing, in parallel, the results of coupled land-atmosphere simulations with the preceding model ECHAM6 hosting JSBACHv3. Large albedo biases appear in both models over ice sheets and in central Asia. The temperate to boreal warm bias observed in simulations with JSBACHv3 largely remained in JSBACHv4, despite the very good agreement with observed LST in the global mean. For the assessment of changes in land water storage, a novel procedure is suggested to compare the gravitational data from the Gravity Recovery And Climate Experiment (GRACE) satellites to simulated TWS. It turns out that the agreement of the changes in the seasonal cycle of TWS is sensitive to the representation of precipitation in the atmosphere model. The LAI is generally too high, which is partly caused by too high soil moisture and also by the parameterization of the phenology itself. The pattern of WUE is, for both models, largely as observed. In India, WUE is too high, probably because JSBACH does not incorporate irrigation in our simulations. WUE differences between the two models can be traced back to differences in precipitation patterns in the two coupled land-atmosphere simulations. For both models, most NPP biases can be associated with biases in water stress, LAI, and FAPAR. In particular, the NPP bias of the Eurasian steppes has switched from positive in JSBACHv3 to negative in JSBACHv4. This difference is mainly caused by weaker precipitation and lower FAPAR of ICON-A-JSBACHv4 in July, which is most probably caused by a feedback loop between too little soil moisture, evaporation, and clouds. While the size and patterns of biases in albedo and LST are largely similar between the two model versions, they are less well correlated for precipitation- and vegetation-related variables like FAPAR. Overall, the biases found in the different assessment variables are either already known from the previous implementation in the Max Planck Institute Earth System Model (MPI-ESM) or have changed because of the coupling with the new atmospheric component ICON-A. Accordingly, this study demonstrates the technically successful completion of the re-implementation of JSBACH into ICON-ESM-V1. As discussed, there is a good perspective on mitigating the biases by an improved representation of the processes
Conditions for the stable adsorption of lipid monolayers to solid surfaces
Lipid monolayers are ubiquitous in biological systems and have multiple roles in biotechnological applications, such as lipid coatings that enhance colloidal stability or prevent surface fouling. Despite the great technological importance of surface-adsorbed lipid monolayers, the connection between their formation and the chemical characteristics of the underlying surfaces has remained poorly understood. Here, we elucidate the conditions required for stable lipid monolayers nonspecifically adsorbed on solid surfaces in aqueous solutions and water/alcohol mixtures. We use a framework that combines the general thermodynamic principles of monolayer adsorption with fully atomistic molecular dynamics simulations. We find that, very universally, the chief descriptor of adsorption free energy is the wetting contact angle of the solvent on the surface. It turns out that monolayers can form and remain thermodynamically stable only on substrates with contact angles above the adsorption contact angle, θadsâ . Our analysis establishes that θads falls into a narrow range of around 60ââ70â in aqueous media and is only weakly dependent on the surface chemistry. Moreover, to a good approximation, θads is roughly determined by the ratio between the surface tensions of hydrocarbons and the solvent. Adding small amounts of alcohol to the aqueous medium lowers θads and thereby facilitates monolayer formation on hydrophilic solid surfaces. At the same time, alcohol addition weakens the adsorption strength on hydrophobic surfaces and results in a slowdown of the adsorption kinetics, which can be useful for the preparation of defect-free monolayers
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