Frequent TMPRSS2-ERG rearrangement in prostatic small cell carcinoma detected by fluorescence in situ hybridization: the superiority of fluorescence in situ hybridization over ERG immunohistochemistry.

Small cell carcinoma of the prostate is both morphologically and immunohistochemically similar to small cell carcinoma of other organs such as the urinary bladder or lung. TMPRSS2-ERG gene fusion appears to be a highly specific alteration in prostatic carcinoma that is frequently shared by small cell carcinoma. In adenocarcinoma, immunohistochemistry for the ERG protein product has been reported to correlate well with the presence of the gene fusion, although in prostatic small cell carcinoma, this relationship is not completely understood. We evaluated 54 cases of small cell carcinoma of the prostate and compared TMPRSS2-ERG gene fusion status by fluorescence in situ hybridization (FISH) to immunohistochemical staining with antibody to ERG. Of 54 cases of prostatic small cell carcinoma, 26 (48%) were positive for TMPRSS2-ERG gene fusion by FISH and 12 (22%) showed overexpression of ERG protein by immunohistochemistry. Of the 26 cases positive by FISH, 11 were also positive for ERG protein by immunohistochemistry. One tumor was positive by immunohistochemistry but negative by FISH. Urinary bladder small cell carcinoma (n = 25) showed negative results by both methods; however, 2 of 14 small cell carcinomas of other organs (lung, head, and neck) showed positive immunohistochemistry but negative FISH. Positive staining for ERG by immunohistochemistry is present in a subset of prostatic small cell carcinomas and correlates with the presence of TMPRSS2-ERG gene fusion. Therefore, it may be useful in confirming prostatic origin when molecular testing is not accessible. However, sensitivity and specificity of ERG immunohistochemistry in small cell carcinoma are decreased compared to FISH

Efeitos da monensina no desempenho de bezerras leiteiras em crescimento Effects of monensin on growing dairy heifers performance

Foi estudado o efeito da suplementação de monensina sobre o desempenho de 32 novilhas Holandesas em crescimento, com peso médio de 84 kg, durante quatro meses . Os animais suplementados com monensina apresentaram maior peso e perímetro torácico aos 90 e 120 dias de experimentação, maior ganho de peso, mostrando um valor de 26,56% maior do que os animais controle. Não foi observada diferença no comprimento corporal e glicose sangüínea, enquanto a altura na cernelha apresentou tendência a valores mais altos nos animais suplementados. Concluiu-se que a suplementação de monensina resultou em maior desenvolvimento dos animais, podendo proporcionar precocidade reprodutiva e produtiva de novilhas leiteiras de reposição.<br>The performance of 32 growing Holstein heifers, with average weight of 84 kg and supplemented with monensin, during four months, was studied. The animals supplemented with monensin presented higher weight and hearth girth at 90 and 120 days of experiment, higher weight gain, with value of 26.56% superior than the control animals. No difference was observed for the corporal length, plasma glucose and the height values. The animals fed monensin presented higher height values. The animals supplemented with monensin showed better development. The use of monensin provided reproductive and productive precocity for the replacement dairy heifers