Bimolecular porous supramolecular networks deposited from solution on layered materials: graphite, boron nitride and molybdenum disulphide

A two-dimensional porous network formed from perylene tetracarboxylic diimide (PTCDI) and melamine may be deposited from solution on the surfaces of highly oriented pyrolytic graphite (HOPG), hexagonal boron nitride (hBN) and molybdenum disulphide (MoS2). Images acquired using high resolution atomic force microscopy (AFM) operating under ambient conditions have revealed that the network forms extended orderedmonolayers (41 lm2) on HOPG and hBN whereas on MoS2 much smaller islands are observed

Single Molecule Biophysics Contributed Talks MEASUREMENTS OF BIOMOLECULAR INTERACTIONS IN PHARMACEUTICAL & IMMUNODIAGNOSTIC SYSTEMS

Recent advances in the immunodiagnostics and pharmaceutical industries have demanded an improved understanding of the molecular interactions that underpin their technologies. For example, the design of more efficacious therapeutic agents requires a detailed knowledge of the proposed target biomolecule or biomolecular interaction. Techniques with the ability to probe single biomolecular interactions, such as the atomic force microscope (AFM), provide a new approach for the direct investigation of such processes at a fundamental level. In previous studies, we have employed AFM force measurements to record interaction forces between the biological molecules employed in a commercial immunoassay system 1−3. More recently, we have employed a similar approach to investigate the influence of the number of epitopes (per protein antigen) in AFM studies of antigen−antibody interactions 4. In addition, interaction forces have been recorded between an anti−human chorionic gonadotrophin antibody (anti−hCG) and a secondary anti− antihCG antibody, and the effect of molecular orientation on the binding force investigated. Such studies require the covalent attachment of biomolecules to the AFM and substrate surface. To this end we have immobilized proteins using either amino−silane chemistry 1 or via a poly (ethyleneglycol) (PEG) polymer tether

Green Chemistry Approach to Surface Decoration: Trimesic Acid Self-Assembly on HOPG

We have investigated trimesic acid (1,3,5-benzenetricarboxylic acid, TMA) adsorption on highly oriented pyrolytic graphite (HOPG) surface from aqueous medium at room temperature. Both atomic force microscopy and scanning tunneling microscopy were utilized to follow the adsorption dynamics and molecular arrangements. We have proposed an optimized green chemistry approach for fabricating trimesic acid monolayer structures on HOPG. A chicken-wire arrangement for adsorbed molecules with an average pore size of 11 ± 1 Å was established and was observed using both scanning techniques. This structure was found to be stable in the ambient for at least two days

In situ surface plasmon resonance analysis of dextran monolayer degradation by dextranase

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