Search CORE

65 research outputs found

Miniaturized engineered heart tissues from hiPSC-derived triple cell type co-cultures to study human cardiac function

Author: Bellin M.
Dostanic M.
Mastrangeli M.
Meer B.J. van
Mummery C.L.
Sarro P.M.
Wiendels M.
Windt L.M.
Publication venue
Publication date: 01/10/2023
Field of study

Human heart tissues grown as three-dimensional spheroids and consisting of different cardiac cell types derived from pluripotent stem cells (hiPSCs) recapitulate aspects of human physiology better than standard two- dimensional models in vitro. They typically consist of less than 5000 cells and are used to measure contraction kinetics although not contraction force. By contrast, engineered heart tissues (EHTs) formed around two flexible pillars, can measure contraction force but conventional EHTs often require between 0.5 and 2 million cells. This makes large-scale screening of many EHTs costly. Our goals here were (i) to create a physiologically relevant model that required fewer cells than standard EHTs making them less expensive, and (ii) to ensure that this miniaturized model retained correct functionality. We demonstrated that fully functional EHTs could be generated from physiologically relevant combinations of hiPSC-derived cardiomyocytes (70%), cardiac fibro-blasts (15%) and cardiac endothelial cells (15%), using as few as 1.6 ×104 cells. Our results showed that these EHTs were viable and functional up to 14 days after formation. The EHTs could be electrically paced in the frequency range between 0.6 and 3 Hz, with the optimum between 0.6 and 2 Hz. This was consistent across three downscaled EHT sizes tested. These findings suggest that miniaturized EHTs could represent a cost-effective microphysiological system for disease modelling and examining drug responses particularly in secondary screens for drug discovery. </p

Leiden University Scholary Publications

Recommended from our members

Gaia Early Data Release 3: The celestial reference frame (Gaia-CRF3)

Author: Abbas U.
Abreu Aramburu A.
Aerts C.
Aguado J.J.
Ajaj M.
Aldea-Montero F.
Altavilla G.
Altmann M.
Alves J.
Anderson R.I.
Andrae R.
Anglada Varela E.
Antoja T.
Arenou F.
Audard M.
Babusiaux C.
Bailer-Jones C.A.L.
Baines D.
Baker S.G.
Bakker J.
Balaguer-Núñez L.
Balbinot E.
Balog Z.
Barache C.
Barbato D.
Barros M.
Barstow M.A.
Bartolomé S.
Bassilana J.-L.
Bastian U.
Bauchet N.
Becciani U.
Bellas-Velidis I.
Bellazzini M.
Benson K.
Berihuete A.
Bernet M.
Berthier J.
Bertone S.
Bianchi L.
Biermann M.
Binnenfeld A.
Blanco-Cuaresma S.
Blomme R.
Boch T.
Bombrun A.
Bossini D.
Bouquillon S.
Bragaglia A.
Bramante L.
Breedt E.
Bressan A.
Brouillet N.
Brown A.G.A.
Brugaletta E.
Bucciarelli B.
Burgess P.W.
Burlacu A.
Busonero D.
Busso G.
Butkevich A.G.
Buzzi R.
Cacciari C.
Caffau E.
Cancelliere R.
Cantat-Gaudin T.
Carballo R.
Carlucci T.
Carnerero M.I.
Carrasco J.M.
Carry B.
Casamiquela L.
Castañeda J.
Castellani M.
Castro-Ginard A.
Cellino A.
Chaoul L.
Charlot P.
Cheek N.
Chemin L.
Chiaramida V.
Chiavassa A.
Chornay N.
Clementini G.
Clotet M.
Comoretto G.
Contursi G.
Cooper W.J.
Cornez T.
Cowell S.
Creevey O.L.
Crifo F.
Cropper M.
Crosta M.
Crowley C.
Cánovas H.
Dafonte C.
Damerdji Y.
Dapergolas A.
David P.
Davidson M.
De Angeli F.
De Bruijne J.H.J.
De Laverny P.
De Luise F.
De March R.
De Ridder J.
De Souza R.
De Teodoro P.
De Torres A.
Del Peloso E.F.
Del Pozo E.
Delbo M.
Delchambre L.
Delgado A.
Delisle J.-B.
Dell'Oro A.
Demouchy C.
Dharmawardena T.E.
Diakite S.
Diaz C. Rios
Diener C.
Distefano E.
Dolding C.
Drimmel R.
Ducourant C.
Enke H.
Esquej P.
Evans D.W.
Eyer L.
Fabre C.
Fabricius C.
Fabrizio M.
Faigler S.
Fedorets G.
Fernique P.
Fernández-Hernández J.
Fienga A.
Figueras F.
Fouesneau M.
Fournier Y.
Fouron C.
Fragkoudi F.
Fraile E.
Frémat Y.
Gai M.
Galluccio L.
Garabato D.
Garcia-Gutierrez A.
Garcia-Reinaldos M.
García-Lario P.
García-Torres M.
Garofalo A.
Garralda Torres N.
Gavel A.
Gavras P.
Gerlach E.
Geyer R.
Giacobbe P.
Gilmore G.
Girona S.
Giuffrida G.
Gomel R.
Gomez A.
González-Núñez J.
González-Santamaría I.
González-Vidal J.J.
Gosset E.
Gracia-Abril G.
Granvik M.
Guerra R.
Guerrier A.
Guillout P.
Guiraud J.
Gutiérrez-Sánchez R.
Guy L.P.
Haigron R.
Halbwachs J.-L.
Hambly N.C.
Harrison D.L.
Hatzidimitriou D.
Hauser M.
Haywood M.
Heiter U.
Helmer A.
Helmi A.
Hernández J.
Hestroffer D.
Hidalgo S.L.
Hilger T.
Hobbs D.
Hodgkin S.T.
Holl B.
Holland G.
Huckle H.E.
Hutton A.
Hładczuk N.
Janben K.
Jansen F.
Jardine K.
Jasniewicz G.
Jean-Antoine Piccolo A.
Jevardat De Fombelle G.
Jiménez-Arranz O.
Jordan S.
Jordi C.
Juaristi Campillo J.
Julbe F.
Karbevska L.
Katz D.
Kervella P.
Khanna S.
Klioner S.A.
Kordopatis G.
Korn A.J.
Kostrzewa-Rutkowska Z.
Krone-Martins A.
Kruszyńska K.
Kun M.
Kóspál A.
Laizeau P.
Lambert S.
Lammers U.L.
Lanza A.F.
Lanzafame A.C.
Lasne Y.
Lattanzi M.G.
Le Campion J.-F.
Lebreton Y.
Lebzelter T.
Leccia S.
Leclerc N.
Lecoeur-Taibi I.
Liao S.
Licata E.L.
Lindegren L.
Lindstrøm H.E.P.
Lister T.A.
Livanou E.
Lobel A.
Lorca A.
Loup C.
Luri X.
Löffler W.
Madrero Pardo P.
Magdaleno Romeo A.
Managau S.
Mann R.G.
Manteiga M.
Marchal O.
Marchant J.M.
Marconi M.
Marcos J.
Marinoni S.
Marocco F.
Marrese P.M.
Marshall D.J.
Marton G.
Martín-Fleitas J.M.
Mary N.
Marín Pina D.
Masana E.
Masip A.
Massari D.
Mastrobuono-Battisti A.
Mazeh T.
McMillan P.J.
Messina S.
Messineo R.
Michalik D.
Mignard F.
Millar N.R.
Mints A.
Moitinho A.
Molina D.
Molinaro R.
Molnár L.
Monari G.
Monguió M.
Montegriffo P.
Montero A.
Mor R.
Mora A.
Morbidelli R.
Morel T.
Morris D.
Mowlavi N.
Muinonen K.
Muraveva T.
Murphy C.P.
Musella I.
Nagy Z.
Nicolas C.
Nienartowicz K.
Noval L.
Nuñez Campos M.
Ocaña F.
Ogden A.
Ordenovic C.
Osborne P.
Osinde J.O.
Pagani C.
Pagano I.
Pailler F.
Palaversa L.
Palicio P.A.
Pallas-Quintela L.
Panahi A.
Pancino E.
Panem C.
Panuzzo P.
Pauwels T.
Payne-Wardenaar S.
Penttilä A.
Peñalosa Esteller X.
Pichon B.
Piersimoni A.M.
Pineau F.-X.
Plachy E.
Plum G.
Poggio E.
Polo L. Martin
Portell J.
Pourbaix D.
Prusti T.
Prša A.
Pulone L.
Racero E.
Ragaini S.
Rainer M.
Raiteri C.M.
Rambaux N.
Ramos P.
Ramos-Lerate M.
Randich S.
Re Fiorentin P.
Recio-Blanco A.
Regibo S.
Reylé C.
Richards P.J.
Riclet F.
Riello M.
Rimoldini L.
Ripepi V.
Riva A.
Rix H.-W.
Rixon G.
Robichon N.
Robin A.C.
Robin C.
Roegiers T.
Roelens M.
Rogues H.R.O.
Rohrbasser L.
Romero-Gómez M.
Roux W.
Rowell N.
Royer F.
Ruz Mieres D.
Rybicki K.A.
Rybizki J.
Sadowski G.
Sagristà Sellés A.
Sahlmann J.
Salguero E.
Samaras N.
Sanchez Gimenez V.
Sanna N.
Santos M. M. S. Marcos
Santoveña R.
Sarasso M.
Sarmiento M.H.
Sarro L.M.
Sartoretti P.
Schultheis M.
Sciacca E.
Seabroke G.M.
Segol M.
Segovia J.C.
Semeux D.
Shahaf S.
Siddiqui H.I.
Siebert A.
Siltala L.
Silvelo A.
Siopis C.
Slezak E.
Slezak I.
Smart R.L.
Smith M.
Snaith O.N.
Solano E.
Solitro F.
Sordo R.
Souami D.
Soubiran C.
Souchay J.
Sozzetti A.
Spagna A.
Spina L.
Spoto F.
Steele I.A.
Steidelmüller H.
Stephenson C.A.
Surdej J.
Szabados L.
Szegedi-Elek E.
Sáez Núñez A.
Ségransan D.
Süveges M.
Tanga P.
Taris F.
Taylor M.B.
Teixeira R.
Teyssier D.
Thévenin F.
Tolomei L.
Tonello N.
Torra F.
Torra J.
Torralba Elipe G.
Trabucchi M.
Tsounis A.T.
Turon C.
Ulla A.
Unger N.
Utrilla E.
Vaillant M.V.
Vallenari A.
Van Dillen E.
Van Leeuwen F.
Van Leeuwen M.
Van Reeven W.
Vanel O.
Vecchiato A.
Viala Y.
Vicente D.
Voutsinas S.
Walton N.A.
Weiler M.
Wevers T.
Wyrzykowski L.
Yoldas A.
Yvard P.
Zhao H.
Zorec J.
Zucker S.
Zwitter T.
Ábrahám P.
Álvarez M.A.
Publication venue: Les Ulis : EDP Sciences
Publication date: 01/01/2022
Field of study

Context. Gaia-CRF3 is the celestial reference frame for positions and proper motions in the third release of data from the Gaia mission, Gaia DR3 (and for the early third release, Gaia EDR3, which contains identical astrometric results). The reference frame is defined by the positions and proper motions at epoch 2016.0 for a specific set of extragalactic sources in the (E)DR3 catalogue. Aims. We describe the construction of Gaia-CRF3 and its properties in terms of the distributions in magnitude, colour, and astrometric quality. Methods. Compact extragalactic sources in Gaia DR3 were identified by positional cross-matching with 17 external catalogues of quasi-stellar objects (QSO) and active galactic nuclei (AGN), followed by astrometric filtering designed to remove stellar contaminants. Selecting a clean sample was favoured over including a higher number of extragalactic sources. For the final sample, the random and systematic errors in the proper motions are analysed, as well as the radio-optical offsets in position for sources in the third realisation of the International Celestial Reference Frame (ICRF3). Results. Gaia-CRF3 comprises about 1.6 million QSO-like sources, of which 1.2 million have five-parameter astrometric solutions in Gaia DR3 and 0.4 million have six-parameter solutions. The sources span the magnitude range G = 13-21 with a peak density at 20.6 mag, at which the typical positional uncertainty is about 1 mas. The proper motions show systematic errors on the level of 12 μas yr-1 on angular scales greater than 15 deg. For the 3142 optical counterparts of ICRF3 sources in the S/X frequency bands, the median offset from the radio positions is about 0.5 mas, but it exceeds 4 mas in either coordinate for 127 sources. We outline the future of Gaia-CRF in the next Gaia data releases. Appendices give further details on the external catalogues used, how to extract information about the Gaia-CRF3 sources, potential (Galactic) confusion sources, and the estimation of the spin and orientation of an astrometric solution

Repositorium für Naturwissenschaften und Technik (TIB Hannover)