QPCR (a), microarray (b) and small RNA sequencing results (c) for <i>MIR204</i> cells.

<p>(a) Average normalized relative quantity (NRQ) for miR-204-5p and miR-204-3p in MIR204WT (CC genotype, black bars, clones 1–3) and MIR204SNP cells (TT genotype, white bars, clones 1–3) normalized for transduction efficiency. (b) Average normalized expression values (NEV) for significantly differently expressed genes between MIR204WT (CC genotype, black bars, clones 4–5) and MIR204SNP (TT genotype, white bars, clones 4–6) cells. (c) Log2 fold changes of miRNAs significantly differently expressed in MIR204SNP cells (clones 4–6) compared to MIR204WT cells (clones 4–5). Error bars in panels (a-b) represent standard deviation of biological replicates, error bars in (c) represent standard error as calculated by DESeq2.</p

MFE secondary structure predictions of hsa-mir-204 and hsa-mir-618 as generated by miRVaS.

<p>(a-b) MFE structure for hsa-mir-204 with variant rs7861254, (a) wild type miRNA, (b) variant miRNA. As the variant is located 107 nt outside the hairpin, flanking regions of 150 nt were included for the predictions. Centroid and MEA predictions with this flank size also showed large structural changes (but different changes), while predictions with flanks of 200 nt resulted in minor changes far away from the hairpin. (c-d) MFE structure of hsa-mir-618 for variant rs2682818, (c) wild type miRNA, (d) variant miRNA. The variant is predicted to induce a shift of the first base of the miR-618 sequence into an internal loop. Flanks of 100 nt were used for this prediction. Predictions were also run for the hairpin with flank sizes of 50 nt, 150 nt and 200 nt and centroid and MEA structures: all predicted the same change within the hairpin. Color scheme: magenta: mature miRNA, orange: seed region, dark blue: terminal loop, cyan: hairpin. The variant is colored in red and indicated by an arrow. Structural changes induced by the variant are colored in black.</p

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

21 juin 18691869/06/21 (A4,N1159)