Undetectable levels of ctDNA following initial treatment are associated with improved survival.

<p>Kaplan–Meier analysis of progression-free (left panel) and overall survival (right panel) between individuals with undetectable (ctDNA = 0; blue lines) and detectable ctDNA (≥ 1; red lines). Significant differences in progression-free survival (p = 0.001) and overall survival (p = 0.0194) between undetectable and detectable groups.</p

Overview of patient enrollment, clinical follow-up and personalized ctDNA analysis.

<p>Overview of patient enrollment, clinical follow-up and personalized ctDNA analysis.</p

Baseline characteristics and pre- and post-hysteroscopy diagnoses of the patient cohort.

<p>Baseline characteristics and pre- and post-hysteroscopy diagnoses of the patient cohort.</p

Summary of mutation allele fractions across patient samples by each mutated gene.

<p>Correlation between the total mutations/gene showed <i>R</i>² = 0.92 (Pearson correlation coefficient) when compared between cell pellet DNA and cfDNA.</p

Cancer driver mutations identified in uterine lavage samples and their comparison to TCGA statistics.

<p>Cancer driver mutations identified in uterine lavage samples and their comparison to TCGA statistics.</p

Flowchart depicting the numbers of patients in each step of the study: enrollment, sample collection, ultra-deep sequencing of samples, and molecular and histopathological classification.

<p>Flowchart depicting the numbers of patients in each step of the study: enrollment, sample collection, ultra-deep sequencing of samples, and molecular and histopathological classification.</p

Clinicopathologic correlates of the seven cancer cases diagnosed by histopathology within the patient cohort.

<p>Clinicopathologic correlates of the seven cancer cases diagnosed by histopathology within the patient cohort.</p

Mutation distributions detected by the 12-gene panel among the study cohort.

<p>Patients are represented along the <i>x</i>-axis first by their histopathologic diagnosis (represented in top bar) then by total mutation number. Mutation types were color-coded hierarchically, displaying the most consequential mutation type (driver, potential driver, passenger) detected at each patient/gene intersection, as some genes carried multiple mutations. NGS-defined mutations validated by dPCR or Sanger sequencing are represented by a black dot. Note: a number of genes had multiple mutations validated.</p

Overview of the study pipeline beginning with collection of uterine lavage fluid at the initiation of hysteroscopy.

<p>Figure designed by Jill Gregory.</p