FastqCleaner: an interactive Bioconductor application for quality-control, filtering and trimming of FASTQ files

Background Exploration and processing of FASTQ files are the first steps in state-of-the-art data analysis workflows of Next Generation Sequencing (NGS) platforms. The large amount of data generated by these technologies has put a challenge in terms of rapid analysis and visualization of sequencing information. Recent integration of the R data analysis platform with web visual frameworks has stimulated the development of user-friendly, powerful, and dynamic NGS data analysis applications.Results This paper presents FastqCleaner, a Bioconductor visual application for both quality-control (QC) and pre-processing of FASTQ files. The interface shows diagnostic information for the input and output data and allows to select a series of filtering and trimming operations in an interactive framework. FastqCleaner combines the technology of Bioconductor for NGS data analysis with the data visualization advantages of a web environment.Conclusions FastqCleaner is an user-friendly, offline-capable tool that enables access to advanced Bioconductor infrastructure. The novel concept of a Bioconductor interactive application that can be used without the need for programming skills, makes FastqCleaner a valuable resource for NGS data analysis.Fil: Roser, Leandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Agüero, Fernán Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Sanchez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentin

Low-Cost Piezoelectric Sensors for Time Domain Load Monitoring of Metallic Structures During Operational and Maintenance Processes

The versatility of piezoelectric sensors in measurement techniques and their performance in applications has given rise to an increased interest in their use for structural and manufacturing component monitoring. They enable wireless and sensor network solutions to be developed in order to directly integrate the sensors into machines, fixtures and tools. Piezoelectric sensors increasingly compete with strain-gauges due to their wide operational temperature range, load and strain sensing accuracy, low power consumption and low cost. This research sets out the use of piezoelectric sensors for real-time monitoring of mechanical strength in metallic structures in the ongoing operational control of machinery components. The behaviour of aluminium and steel structures under flexural strength was studied using piezoelectric sensors. Variations in structural behaviour and geometry were measured, and the load and μstrains during operational conditions were quantified in the time domain at a specific frequency. The lead zirconium titanate (PZT) sensors were able to distinguish between material types and thicknesses. Moreover, this work covers frequency selection and optimisation from 20 Hz to 300 kHz. Significant differences in terms of optimal operating frequencies and sensitivity were found in both structures. The influence of the PZT voltage applied was assessed to reduce power consumption without signal loss, and calibration to μstrains and loads was performed.This research was funded by Basque Government, grant number KK-2019/00051-SMARTRESNAK and by the European Commission, grant number 869884- RECLAIM

Depletion of the SR-related protein TbRRM1 leads to cell cycle arrest and apoptosis-like death in Trypanosoma brucei

Arginine-Serine (RS) domain-containing proteins are RNA binding proteins with multiple functions in RNA metabolism. In mammalian cells this group of proteins is also implicated in regulation and coordination of cell cycle and apoptosis. In trypanosomes, an early branching group within the eukaryotic lineage, this group of proteins is represented by 3 members, two of them are SR proteins and have been recently shown to be involved in rRNA processing as well as in pre-mRNA splicing and stability. Here we report our findings on the 3rd member, the SR-related protein TbRRM1. In the present study, we showed that TbRRM1 ablation by RNA-interference in T. brucei procyclic cells leads to cell-cycle block, abnormal cell elongation compatible with the nozzle phenotype and cell death by an apoptosis-like mechanism. Our results expand the role of the trypanosomal RS-domain containing proteins in key cellular processes such as cell cycle and apoptosis-like death, roles also carried out by the mammalian SR proteins, and thus suggesting a conserved function in this phylogenetically conserved protein family.Fil: Levy, Gabriela Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Bañuelos, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Níttolo, Analía Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Mendiondo, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Moretti, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Tekiel, Valeria Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Sanchez, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentin

Ancient Landscapes of Uruguay

In this chapter, based on the available geological information, a model for the genesis and evolution of the Uruguayan landscape is proposed. A structural framework of the landscape evolution is provided and the record of such evolution in the most representative geological units is considered. A brief summary of the Uruguayan geology and its location in the regional context is performed, from Precambrian to Cenozoic times.From the analysis of the geological record, it may be observed that the climate was very arid during part of the Jurassic and the Early Cretaceous. Together with the lava flows of the Arapey Formation, the climate became less arid as the Gondwana continents were becoming apart from each other. However, the geological record suggests that semiarid climates were still prevailing. In the Middle Cretaceous, semiarid and wetter climates progressively alternated, until the Early Tertiary, when very wet and warm conditions were established, in coincidence with the ?Paleocene Eocene Thermal Maximum (PETM)?, followed by semiarid climates in the Oligocene, wetter conditions in the Miocene and semiarid again in the Pliocene, with alternating semiarid and humid conditions during the entire Quaternary.On the basis of the paleoclimatic evolution, the development of relief is discussed, considering as bases for the analysis the different morphostructural units in which the country is divided. Due to their size, shape and location (passive margin) of Uruguay, climate uniformity is assumed for each period throughout the entire territory. It is also assumed that the surfaces around elevations of 500 meters correspond to relicts of probably pre-Cretaceous etchplains, strongly denudated, which are observed only in the surroundings of Aiguá.The landforms situated below the oldest surfaces, for instance those below 320 m a.s.l. in the Easthern Hills Regions (Sierra del Este), correspond to a new generation of geomorphological surfaces that may be considered of Cretaceous age, according to the information presently available. This surface may be correlated with the oldest surface developed on top of the lava flows of the Arapey Formation.The extremely warm and wet climate of the Eocene prepared the conditions for the planation processes that covered most of the Uruguayan territory during the Oligocene, generating pediplains which were later reworked during the Late Cenozoic, up to the Quaternary, generating a landscape of smooth hills.The morphogenetic potential of each morphostructural region determined the available energy of the resulting landscape, being this at a minimum in the Santa Lucía Basin, which continued to be under subsidence condition until the Tertiary, and almost non-existant in the Laguna Merín Basin, where subsidence remains active until the Holocene.Fil: Panario, Daniel. Universidad de la República; UruguayFil: Gutierrez, Ofelia. Universidad de la República; UruguayFil: Bettucci Sanchez, Leda. Universidad de la República; UruguayFil: Peel, Elena. Universidad de la República; UruguayFil: Oyhantcabal, Pedro. Universidad de la República; UruguayFil: Rabassa, Jorge Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentin

First data of the Colombia Lightning Mapping Array - COLMA

The first data set of VHF lightning mapping using a Lightning Mapping Array system - LMA in a tropical region is presented in this paper. Six sensors were installed at the north of Colombia near Santa Marta city. Since the installation of the LMA network in 2015, up to 7000 intra-cloud (IC) discharges from September to November 2015 have been analyzed. The data suggests that, the electrical charge distribution in tropical thunderstorms shows higher vertical development reaching higher altitudesPreprin

Changes in and the mediating role of physical activity in relation to active school transport, fitness and adiposity among Spanish youth: the UP&DOWN longitudinal study

Background Longitudinal changes in child and adolescent active school transport (AST), and the mediating role of different intensities of daily physical activity (PA) levels in relation to AST and physical fitness and adiposity indicators is unclear. This study aimed to: 1) describe longitudinal changes in AST, light PA (LPA), moderate- to vigorous-intensity PA (MVPA), physical fitness and adiposity indicators over three time-points; and 2) investigate the mediating role of LPA and MVPA levels on associations between AST and physical fitness and adiposity indicators over three time-points among children and adolescents. Methods This longitudinal study comprised 1646 Spanish children and adolescents (48.8% girls, mean age 12.5 years +/- 2.5) at baseline, recruited from schools in Cadiz and Madrid. Mode of commuting to school was self-reported at baseline (T0, 2011-12), 1-year (T1) and 2-year follow-up (T2). PA was assessed using accelerometers. Handgrip strength, standing long jump and cardiorespiratory fitness (CRF) assessed physical fitness. Height, weight, body mass index, waist circumference, and triceps and subscapular skinfold thickness were measured. Multilevel linear regression analyses assessed changes in AST, PA levels, physical fitness and adiposity indicators over three time-points (T0-T1-T2). Additionally, longitudinal path analysis (n = 453; mean age [years] 12.6 +/- 2.4) was used to test the mediating effects of LPA and MVPA levels on the association between AST and physical fitness and adiposity indicators. Results Multilevel analyses observed decreases in LPA between T0-T1 (beta = - 11.27; p < 0.001) and T0-T2 (beta = - 16.27; p < 0.001) and decreases in MVPA between T0-T2 (beta = - 4.51; p = 0.011). Moreover, changes over time showed increases in handgrip between T0-T1 (beta = 0.78; p = 0.028) and T0-T2 (beta = 0.81; p = 0.046). Path analyses showed that AST was directly positively associated with MVPA at T1 (all, beta approximate to 0.33; p < 0.001). MVPA at T1 mediated associations between AST and CRF at T2 (beta = 0.20; p = 0.040), but not the other outcomes. LPA did not mediate any associations. Conclusions Results from longitudinal path analysis suggest that participation in more AST may help attenuate declines in MVPA that typically occur with age and improve CRF. Therefore, we encourage health authorities to promote AST, as a way to increase MVPA levels and CRF among youth

The Trypanosoma brucei RNA-Binding protein TbRRM1 is involved in the transcription of a subset of RNA Pol II-Dependent genes

It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes. We showed that TbRRM1 is recruited throughout the PTU, with a higher presence on genes than intergenic regions. However, its depletion leads both to the decrease of nascent RNA and to chromatin compaction only of regions located distal to the main transcription start site. These findings suggest that TbRRM1 facilitates the RNA Pol II transcriptional elongation step by collaborating to maintain an open chromatin state in particular regions of the genome. Interestingly, the SL-RNA genes do not recruit TbRRM1 and, after TbRRM1 knockdown, nascent SL-RNAs accumulate while the chromatin state of these regions remains unchanged. Although it was previously suggested that TbRRM1 could regulate RNA Pol II-driven genes, we provide here the first experimental evidence which involves TbRRM1 to transcriptional regulation.Fil: Bañuelos, Carolina Paula. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Levy, Gabriela Vanesa. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Níttolo, Analía Gabriela. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Roser, Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentin

Transmigration of Trypanosoma cruzi trypomastigotes through 3D cultures resembling a physiological environment

To disseminate and colonise tissues in the mammalian host, Trypanosoma cruzi trypomastogotes should cross several biological barriers. How this process occurs or its impact in the outcome of the disease is largely speculative. We examined the in vitro transmigration of trypomastigotes through three-dimensional cultures (spheroids) to understand the tissular dissemination of different T. cruzi strains. Virulent strains were highly invasive: trypomastigotes deeply transmigrate up to 50 μm inside spheroids and were evenly distributed at the spheroid surface. Parasites inside spheroids were systematically observed in the space between cells suggesting a paracellular route of transmigration. On the contrary, poorly virulent strains presented a weak migratory capacity and remained in the external layers of spheroids with a patch-like distribution pattern. The invasiveness—understood as the ability to transmigrate deep into spheroids—was not a transferable feature between strains, neither by soluble or secreted factors nor by co-cultivation of trypomastigotes from invasive and non-invasive strains. Besides, we demonstrated that T. cruzi isolates from children that were born congenitally infected presented a highly migrant phenotype while an isolate from an infected mother (that never transmitted the infection to any of her children) presented significantly less migration. In brief, we demonstrated that in a 3D microenvironment each strain presents a characteristic migration pattern that can be associated to their in vivo behaviour. Altogether, data presented here repositionate spheroids as a valuable tool to study host–pathogen interactions.Fil: Rodriguez, Matias Exequiel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Rizzi, Mariana. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Caeiro, Lucas Daniel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Masip, Yamil Ezequiel. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Perrone, Alina Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Daniel Oscar. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Bua, Jacqueline Elena. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tekiel, Valeria Sonia. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentin

Análisis de residuos de disparo mediante fluorescencia de Rayos X

Varias técnicas analíticas están disponibles para que los científicos forenses realicen la reconstrucción de un hecho. Entre ellas, el análisis de la presencia de elementos típicos de residuos de disparos, como Pb, Ba y Sb, es lo más frecuente. La investigación de su presencia en el cuerpo de la víctima o de un sospechoso puede proporcionar información crucial sobre el incidente. En este trabajo proponemos el uso de la fluorescencia de rayos X para la detección de residuos de disparo en piel. El análisis se centró en la detección de los elementos antes mencionados, en las manos y la cara de un tirador. Se realizó un experimento en la sala de disparo (AIC-SR), utilizando stubs de carbon comerciales para tomar muestras de las manos y el rostro de él tirador antes y después de disparar una pistola 9 mm. Los resultados indican una clara correlación entre las muestras tomadas de ambas manos después de disparar una pistola 9 mm en relación con la presencia de Pb y Ba. El instrumento XRF demostró ser una herramienta rápida y sencilla para detectar componentes inorgánicos como lo son los residuos de disparo en las manos de un tirador.Several analytical techniques are available for forensic scientists to perform the reconstruction of a shooting incident. Among them, the analysis of gunshot residues typical elements, as Pb, Ba and Sb, are the most frequent. Investigation of its presence on the body from the victim or a suspect can provide crucial information regarding the incident. In this work we propose the use of X-ray fluorescence for detecting gunpowder residues on the skin. The analysis was focused in the relationship of these elements, mainly on the hands and face of a handgun shooter. An experiment was performed in the forensic laboratory shooting room, using commercial carbon stubs for sampling the hand and face skin of a shooter firing a 9 mm pistol. Results indicate a clear correlation between samples taken from both hands after firing a 9 mm pistol in relationship to Pb an Ba presence. The XRF instrument proved to be a quick and easy tool to detect inorganic components as a screening for GSR on hands.Fil: Saran, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Gobierno de la Provincia de La Pampa. Ministerio Público. Agencia de Investigación Científica; ArgentinaFil: Gualpa, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; ArgentinaFil: Much, Diego Gabriel. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; ArgentinaFil: Sanchez Ormachea, Jesica Pamela. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; ArgentinaFil: Wilberger, Daniel Oscar. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; Argentina. Universidad Siglo 21; ArgentinaFil: Merini, Luciano Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; Argentin

Evaluación de estado físico, antropométrico y biomarcadores en árbitros de fútbol del departamento del Quindío.(Original)

Objective: to determine the anthropometric profile, the physical condition and the behavior of biomarkers associated with physical load in ranked soccer referees from Quindío’s region. Methodology: there was participation of 14 referees (9 assistants and 5 main referees); whose body composition was estimated through the 4-component model, in addition to health indices as physical condition variables which were evaluated: flexibility, strength, speed and resistance; this last variable was measured through the intermittent level 1 YOYO test, where maximum heart rate and the recovery associated with it were measured, the subjective perceived exertion (RPE), mass and urine specific gravity (USG) to determine the state of hydration in the test. Results: Among the main results, it was found that, with respect to the health marker groups, the referees were in the ranges considered normal, in the characterization of the physical condition, no statistical significance was found between (assistant referees and main referees). Regarding the behavior of physical load markers, it was observed that the maximum heart rate was 188 and 181 bpm on average for main and assistant referees, respectively, without statistical significance. The recovery after the stimulus occurred in a decreasing way and in each of the shots showed statistical significance with respect to the previous one. The RPE was classified as a very heavy effort. Finally, regarding the state of hydration, it was shown that, although the mass, pre and post, presented a statistically significant difference, the same did not occur with respect to USG. Conclusion: although the main referees presented better physical condition results than the assistant referees, these differences were not statistically significant. Regarding physical load biomarkers, they behaved similarly in both groups.Objetivo: determinar el perfil antropométrico, de condición física y el comportamiento de biomarcadores asociados a la carga física en árbitros de fútbol escalafonados del departamento de Quindío. Metodología: participaron 14 árbitros (9 asistentes y 5 centrales); a los cuales se les estimó la composición corporal a través del modelo de 4 componentes, además índices de salud; como variables de condición física fueron evaluadas flexibilidad, fuerza, velocidad y resistencia, esta última a través del test YOYO de recuperación intermitente nivel 1, en este test de resistencia se midió la frecuencia cardiaca máxima y la recuperación asociada a esta, también se evaluó la percepción subjetiva del esfuerzo (PSE), masa y gravedad específica de la orina (GEO) para determinar el estado de hidratación en el test. Resultados:Dentro de los principales resultados se encontró que, con respecto a los marcadores de salud, los árbitros se encontraron en los rangos considerados como normales, en la caracterización de la condición física, no se encontró significancia estadística entre grupos (árbitros asistentes y árbitros centrales). En relación con el comportamiento de marcadores de carga física, se pudo apreciar que la frecuencia cardiaca máxima fue de 188 y 181 ppm en promedio para árbitros centrales y asistentes respectivamente, sin significancia estadística. La recuperación post estímulo se dio de forma decreciente y en cada una de las tomas mostró significancia estadística con respecto a la anterior. La PSE se clasificó como un esfuerzo muy pesado. Finalmente, con respecto al estado de hidratación se observó que, aunque la masa, pre y post presentó diferencia estadísticamente significativa, no ocurrió lo mismo con respecto a GEO. Conclusión:aunque los árbitros centrales presentaron mejores resultados de condición física que los árbitros asistentes, estas diferencias no fueron estadísticamente significativas. Con respecto a los biomarcadores de carga física, estos se comportaron de manera similar en ambos grupos