ACL Risk Data Comparison And Turnout Analysis Of Female Dancers Trained In Ballet And Female Traditional Jumping Sport Athletes

INTRODUCTION: An increase in participation in females sports has created an increase of female athletes at risk for injuring their Anterior Cruciate Ligament(ACL)[12,21,29,44]. Traditional jumping sports have the highest rate of non contact ACL injuries, due to the use of movements of cutting, pivoting and landing on one foot[5,8,32,33,38]. ACL injuries can also be attributed to neuromuscular deficits such as the ‘Ligament Dominance Theory’, ‘Quadricep Dominance Theory’, ‘Trunk Dominance Theory’ and the ‘Leg Dominance Theory’[24,33]. The neuromuscular deficits are muscle strength, power or activation patterns that can cause an individual to have an increased risk of ACL injury[33]. Female traditional jumping sport athletes have been associated with being at a higher risk of ACL injury than their male counterparts due to anatomical, hormonal and neuromuscular differences[2,8,24,28,32,37,38,44,47]. However, female dancers trained with ballet have a lower risk of ACL injury than their female athlete counterparts, but also have a similar ACL injury to their male dancing counterparts [28,37,45,47]. METHODS: This study analyzed six papers that compared the lower body biomechanics of female traditional jumping sport athletes to female dancers trained in ballet. The results of the measurement of this study will be placed into a chart to compare the results of each study to each other, to confirm the results of the comparison between the two populations. The next part of this study will examine unused turnout angle data collected from a previous thesis performed by Ashley Tornio. The data was taken from 20 participants, 15 female traditional jumping sport athletes and 5 female dancers trained in ballet. The averages of these two groups will be compared using an f test to determine differences in the turnout capabilities of each group. RESULTS: The results of the data comparison found only six comparable measurements between the 6 papers. The papers were in agreement that female traditional jumping sport athlete had greater hip adduction moments and trunk forward flexion than female dancers trained in ballet. The papers were also in agreement that there was no statistically significant difference in the knee stiffness between the two populations. There was no consensus for the results of knee valgus angle, knee rotation, muscle activation or leg stiffness between the six papers. For the turnout angle f test, female traditional jumping sport athletes had an average turnout angle of 120.5 degrees and the female dancers trained in ballet had an average turnout angle of 141.2 degrees. It was found that the there was no statistically significant difference between the two populations at the 95% confidence level. However, there was a statistically significant difference between the two populations average turnout at a reduced confidence level of 80%. The DISCUSSION: The limiting number of studies which compare female traditional jumping sport athletes and female dancers trained in ballet, were unable to form consensus on the difference between the biomechanics of each group during a landing task. The turnout angle data was also limited in the number of participants and a valid conclusion was unable to be made determining the ability to use the turnout angle as an indicator for risk of ACL injury. There needs to be continued research on the comparison of the female traditional jumping sport athletes and female dancers trained in ballet to determine the biomechanical advantages female dancers have for protection of the ACL

Results of the 2007-2008 Illinois Hunter Harvest Survey

Grant/Contract No: W-112-R-17A random sample of 3,000 potential hunters was selected from among 2007 Illinois Habitat Stamp and hunting license purchasers. An 8-page questionnaire was successfully mailed to 2,906 of the 3,000 individuals. We received 1,625 returned questionnaires, 1,620 of which were usable, for a 56% response rate. Comparisons of harvest estimates from the 2006-2007 season to the 2007-2008 season suggest harvest decreased for 10 game animal categories (rabbit, quail, pheasant, dove, gray partridge, fox squirrel, gray squirrel, red fox, gray fox, and opossum), and increased for 3 categories (woodcock, raccoon, and coyote). License sales increased 0.4% from 282,000 sold for the 2006 season to 283,000 sold in the 2007 season. This represents a recovery in license sales of less than 1% since the lowest level on record (since 1938) in 2005. Low license sales may be related to increasing difficulty in finding land to hunt. Resident hunters pursuing deer (61%) and small game species (51%) reported little difficulty in gaining access to hunting lands. However, all other seasons were associated with more difficulty in accessing land. Efforts should be undertaken to ensure that hunters are aware of the locations of public hunting lands associated with these seasons. Few residents reported leasing land (4%) or hiring outfitters (1%) to access land.INHS Technical Report Prepared for Illinois Department of Natural Resources; Illinois Natural History Surve

Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe

Background DNA replication initiates on defined genome sites, termed origins. Origin usage appears to follow common rules in the eukaryotic organisms examined to date: all chromosomes are replicated from multiple origins, which display variations in firing efficiency and are selected from a larger pool of potential origins. To ask if these features of DNA replication are true of all eukaryotes, we describe genome-wide origin mapping in the parasite Leishmania. Results Origin mapping in Leishmania suggests a striking divergence in origin usage relative to characterized eukaryotes, since each chromosome appears to be replicated from a single origin. By comparing two species of Leishmania, we find evidence that such origin singularity is maintained in the face of chromosome fusion or fission events during evolution. Mapping Leishmania origins suggests that all origins fire with equal efficiency, and that the genomic sites occupied by origins differ from related non-origins sites. Finally, we provide evidence that origin location in Leishmania displays striking conservation with Trypanosoma brucei, despite the latter parasite replicating its chromosomes from multiple, variable strength origins. Conclusions The demonstration of chromosome replication for a single origin in Leishmania, a microbial eukaryote, has implications for the evolution of origin multiplicity and associated controls, and may explain the pervasive aneuploidy that characterizes Leishmania chromosome architecture

Change in hematologic indices over time in pediatric inflammatory bowel disease treated with azathioprin

Azathioprine leads to changes in mean corpuscular volume (MCV) and white blood cell (WBC) indices reflecting efficacy or toxicity. Understanding the interactions between bone marrow stem cells and azathioprine could highlight abnormal response patterns as forerunners for hematologic malignancies. This study gives a statistical description of factors influencing the relationship between MCV and WBC in children with inflammatory bowel disease treated with azathioprine. We found that leukopenia preceded macrocytosis. Macrocytosis is therefore not a good predictor of leukopenia. Further studies will be necessary to determine the subgroup of patients at increased risk of malignancies based on bone marrow response. © 2010 Soman et al., publisher and licensee Adis Data Information BV

Isolated IgG2 deficiency is an independent risk factor for exacerbations in bronchiectasis

BACKGROUND: Immunoglobulin G (IgG) subclass 2 deficiency is the most frequent IgG subclass deficiency identified in patients with bronchiectasis, but its clinical significance is not known. AIM: To analyse if bronchiectasis patients with isolated IgG2 deficiency at risk of recurrent exacerbations and/or hospitalization? Do patients with IgG2 deficiency have worse disease progression? DESIGN AND METHODS: This is a retrospective study (2015–20) exploring independent risk factors for recurrent exacerbations (3 or more per year) and/or hospitalization with bronchiectasis exacerbations using multivariable models using binary logistic regression. There was no patient with IgG deficiency, IgG 1, 3 or 4 deficiency, or IgA or IgM deficiency included. In this model, the authors included: serum IgG2 level; lung function; body mass index; MRC breathlessness scale; age; sex; number of bronchiectatic lobes; bacterial colonization; comorbidities; and the use of long-term immunosuppressant drugs or antibiotics for more than 28 days. Analysing 2-year longitudinal data, one-way ANOVA and Mann–Whitney U-test were used to compare bronchiectasis severity between patients with different IgG2 levels. RESULTS: Serum IgG2 levels (4.45 g/l) (P = 0.003, 0.013). CONCLUSION: Reduced IgG2 levels were an independent predictor for bronchiectasis exacerbations and have increased disease progression

Results from 10 Years of a CBT Pain Self-Management Outpatient Program for Complex Chronic Conditions

Background. Traditional unimodal interventions may be insufficient for treating complex pain, as they do not address cognitive and behavioural contributors to pain. Cognitive Behavioural Therapy (CBT) and physical exercise (PE) are empirically supported treatments that can reduce pain and improve quality of life. Objectives. To examine the outcomes of a pain self-management outpatient program based on CBT and PE at a rehabilitation hospital in Toronto, Ontario. Methods. The pain management group (PMG) consisted of 20 sessions over 10 weeks. The intervention consisted of four components: education, cognitive behavioural skills, exercise, and self-management strategies. Outcome measures included the sensory, affective, and intensity of pain experience, depression, anxiety, pain disability, active and passive coping style, and general health functioning. Results. From 2002 to 2011, 36 PMGs were run. In total, 311 patients entered the program and 214 completed it. Paired t-tests showed significant pre- to posttreatment improvements in all outcomes measured. Patient outcomes did not differ according to the number or type of diagnoses. Both before and after treatment, women reported more active coping than men. Discussion. The PMGs improved pain self-management for patients with complex pain. Future research should use a randomized controlled design to better understand the outcomes of PMGs

Biophysical and Structural Characterization of Novel RAS-Binding Domains (RBDs) of PI3Kα and PI3Kγ

Phosphatidylinositol-3-kinases (PI3Ks) are lipid kinases that phosphorylate phosphatidylinositol 4,5-bisphosphate to generate a key lipid second messenger, phosphatidylinositol 3,4,5-bisphosphate. PI3Kα and PI3Kγ require activation by RAS proteins to stimulate signaling pathways that control cellular growth, differentiation, motility and survival. Intriguingly, RAS binding to PI3K isoforms likely differ, as RAS mutations have been identified that discriminate between PI3Kα and PI3Kγ, consistent with low sequence homology (23%) between their RAS binding domains (RBDs). As disruption of the RAS/PI3Kα interaction reduces tumor growth in mice with RAS- and epidermal growth factor receptor driven skin and lung cancers, compounds that interfere with this key interaction may prove useful as anti-cancer agents. However, a structure of PI3Kα bound to RAS is lacking, limiting drug discovery efforts. Expression of full-length PI3K isoforms in insect cells has resulted in low yield and variable activity, limiting biophysical and structural studies of RAS/PI3K interactions. This led us to generate the first RBDs from PI3Kα and PI3Kγ that can be expressed at high yield in bacteria and bind to RAS with similar affinity to full-length PI3K. We also solved a 2.31 Å X-ray crystal structure of the PI3Kα-RBD, which aligns well to full-length PI3Kα. Structural differences between the PI3Kα and PI3Kγ RBDs are consistent with differences in thermal stability and may underly differential RAS recognition and RAS-mediated PI3K activation. These high expression, functional PI3K RBDs will aid in interrogating RAS interactions and could aid in identifying inhibitors of this key interaction

Change in hematologic indices over time in pediatric inflammatory bowel disease treated with azathioprine

Azathioprine leads to changes in mean corpuscular volume (MCV) and white blood cell (WBC) indices reflecting efficacy or toxicity. Understanding the interactions between bone marrow stem cells and azathioprine could highlight abnormal response patterns as forerunners for hematologic malig-nancies. This study gives a statistical description of factors influencing the relationship between MCV and WBC in children with inflammatory bowel disease treated with azathioprine. We found that leukopenia preceded macro¬cytosis. Macrocytosis is therefore not a good predictor of leukopenia. Further studies will be necessary to determine the subgroup of patients at increased risk of malignancies based on bone marrow response

Fate of Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes During Curing and Drying of Beef Bresaola

Manufacturing dry-cured meat products without a thermal lethality step is a growing trend for charcuterie companies in the United States. The United States Department of Agriculture Food Safety and Inspection Service requires that hazards for ready-to-eat meat products be addressed with a scientifically valid Hazard Analysis Critical Control Point system. Because little validation literature exists for these products, an experiment was designed to investigate the safety of beef bresaola. The objective of this study was to determine the reduction of Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes during curing and drying of bresaola.Prior to curing, whole beef semitendinosus muscle was inoculated with a mixed culture containing 3 strains each of E. coli O157:H7, Salmonella spp., and L. monocytogenes, allowed to air dry (30 min at 23°C), sprayed with a 2.5% Beefxide antimicrobial treatment (Birko Corp., Henderson, CO), and allowed to sit overnight in a walk-in cooler (2°C–4°C). Cure (NaNO3and NaNO2) and salt were applied to the beef surface 24 h after the antimicrobial treatment, and the beef was cured for 28 d (2°C–4°C). Following curing, a proprietary spice mixture was surface coated, and each piece was stuffed into beef casings (115–130 mm). The stuffed bresaola pieces were hung and allowed to dry for 35 d to a target water activity < 0.92 (13.63°C ± 2°C; relative humidity 68% ± 7%). Pathogen populations and water activity were analyzed on days 0, 1, and 2 and then weekly until day 65 of the study. Final reductions of 5.97, 5.98, and 5.44 log10 colony-forming units (CFU)/cm2 were achieved on day 65 for E. coli, Salmonella spp., and L. monocytogenes, respectively. During the entire curing and drying process, populations of each species never increased by more than 0.5 log10 CFU/cm2. The critical parameters used to cure and dry this product are sufficient to achieve the minimum 5 log10 CFU/cm2 reduction of each pathogen as required by the United States Department of Agriculture Food Safety and Inspection Service to validate process safety