Pengaruh Penanganan Pasca Panen Terhadap Mutu Komoditas Hortikultura

Postharvest technologies are needed for horticultura commodities because its perishable characteristic. The technics are included cleaning, curing, sorting or grading, degreening, packing, and cooling. Especially in cooling application, there are two variables influenced characteristic of product i.e temperature and hummidity. Temperature of 0-7 ºC and hummidity of 90-95% are recommended to be used because very effective to decrease of water losses, material dextructive of bacterial, growth root, respiration, texture and color changing

Aging of the Nonlinear Optical Susceptibility of colloidal solutions

Using Z-scan and dynamic light scattering measurements we investigate the nonlinear optics response of a colloidal solution undergoing dynamics slowing down with age. We study the high optical nonlinearity of an organic dye (Rhodamine B) dispersed in a water-clay (Laponite) solution, at different clay concentrations (2.0 wt% - 2.6 wt%), experiencing the gelation process. We determine the clay platelets self diffusion coefficient and, by its comparison with the structural relaxation time, we conclude that the gelation process proceeds through the structuring of interconnecting clay platelets network rather than through clusters growth and aggregation.Comment: 4 figures, 4 page

Strong negative self regulation of Prokaryotic transcription factors increases the intrinsic noise of protein expression

Background Many prokaryotic transcription factors repress their own transcription. It is often asserted that such regulation enables a cell to homeostatically maintain protein abundance. We explore the role of negative self regulation of transcription in regulating the variability of protein abundance using a variety of stochastic modeling techniques. Results We undertake a novel analysis of a classic model for negative self regulation. We demonstrate that, with standard approximations, protein variance relative to its mean should be independent of repressor strength in a physiological range. Consequently, in that range, the coefficient of variation would increase with repressor strength. However, stochastic computer simulations demonstrate that there is a greater increase in noise associated with strong repressors than predicted by theory. The discrepancies between the mathematical analysis and computer simulations arise because with strong repressors the approximation that leads to Michaelis-Menten-like hyperbolic repression terms ceases to be valid. Because we observe that strong negative feedback increases variability and so is unlikely to be a mechanism for noise control, we suggest instead that negative feedback is evolutionarily favoured because it allows the cell to minimize mRNA usage. To test this, we used in silico evolution to demonstrate that while negative feedback can achieve only a modest improvement in protein noise reduction compared with the unregulated system, it can achieve good improvement in protein response times and very substantial improvement in reducing mRNA levels. Conclusions Strong negative self regulation of transcription may not always be a mechanism for homeostatic control of protein abundance, but instead might be evolutionarily favoured as a mechanism to limit the use of mRNA. The use of hyperbolic terms derived from quasi-steady-state approximation should also be avoided in the analysis of stochastic models with strong repressors

Systematic study of the pp -> pp omega reaction

A systematic study of the production of omega-mesons in proton-proton-collisions was carried out in a kinematically complete experiment at three excess energies(epsilon= 92, 128, 173MeV). Both protons were detected using the large-acceptance COSY-TOF spectrometer at an external beam line at the Cooler Synchrotron COSY at Forschungszentrum J\"ulich. The total cross section, angular distributions of both omega-mesons and protons were measured and presented in various reference frames such as the overall CMS, helicity and Jackson frame. In addition, the orientation of the omega-spin and invariant mass spectra were determined. We observe omega-production to take place dominantly in Ss and Sp final states at epsilon = 92, 128 MeV and, additionally, in Sd at epsilon= 173 MeV. No obvious indication of resonant omega-production via N^*-resonances was found, as proton angular distributions are almost isotropic and invariant mass spectra are compatible with phase space distributions. A dominant role of ^3P_1 and ^1S_0 initial partial waves for omega-production was concluded from the orientation of the decay plane of the omega-meson. Although the Jackson angle distributions in the omega-p-Jackson frame are anisotropic we argue that this is not an indication of a resonance but rather a kinematical effect reflecting the anisotropy of the omega angular distribution. The helicity angle distribution in the omega-p-helicity frame shows an anisotropy which probably reflects effects of the omega angular momenta in the final state; this observable may be, in addition to the orientation of the omega decay plane, the most sensitive one to judge the validity of theoretical descriptions of the production process.Comment: 17 pages, 16 figures, accepted for publication in EPJ

Measurement of the Eta Production in Proton Proton Collisions with the COSY Time of Flight Spectrometer

The reaction pp -> pp eta was measured at excess energies of 15 and 41 MeV at an external target of the Juelich Cooler Synchrotron COSY with the Time of Flight Spectrometer. About 25000 events were measured for the excess energy of 15 MeV and about 8000 for 41 MeV. Both protons of the process pp eta were detected with an acceptance of nearly 100% and the eta was reconstructed by the missing mass technique. For both excess energies the angular distributions are found to be nearly isotropic. In the invariant mass distributions strong deviations from the pure phase space distributions are seen.Comment: 15 pages, 14 figures, 4 table

On the Production of π+π+\pi^+\pi^+ Pairs in pp Collisions at 0.8 GeV

Data accumulated recently for the exclusive measurement of the $pp\to pp\pi^+\pi^-$ reaction at a beam energy of 0.793 GeV using the COSY-TOF spectrometer have been analyzed with respect to possible events from the $pp \to nn\pi^+\pi^+$ reaction channel. The latter is expected to be the only $\pi\pi$ production channel, which contains no major contributions from resonance excitation close to threshold and hence should be a good testing ground for chiral dynamics in the $\pi\pi$ production process. No single event has been found, which meets all conditions for being a candidate for the $pp \to nn \pi^+\pi^+$ reaction. This gives an upper limit for the cross section of 0.16 $\mu$b (90% C.L.), which is more than an order of magnitude smaller than the cross sections of the other two-pion production channels at the same incident energy

Production of Lambda and Sigma^0 hyperons in proton-proton collisions

This paper reports results on simultaneous measurements of the reaction channels pp -> pK+\Lambda and pp -> pK+\Sigma^0 at excess energies of 204, 239, and 284 MeV (\Lambda) and 127, 162, and 207 MeV (\Sigma^0). Total and differential cross sections are given for both reactions. It is concluded from the measured total cross sections that the high energy limit of the cross section ratio is almost reached at an excess energy of only about 200 MeV. From the differential distributions observed in the overall CMS as well as in the Jackson and helicity frames, a significant contribution of interfering nucleon resonances to the \Lambda production mechanism is concluded while resonant \Sigma^0-production seems to be of lesser importance and takes place only through specific partial waves of the entrance channel. The data also indicate that kaon exchange plays a minor role in the case of \Lambda- but an important role for \Sigma^0-production. Thus the peculiar energy dependence of the \Lambda-to-\Sigma^0 cross section ratio appears in a new light as its explanation requires more than mere differences between the p\Lambda and the p\Sigma^0 final state interaction. The data provide a benchmark for theoretical models already available or yet to come.Comment: 18 pages, 10 figures; accepted by The European Physical Journal A (EPJ A

Single-Pion Production in pp Collisions at 0.95 GeV/c (II)

The single-pion production reactions $pp\to d\pi^+$, $pp\to np\pi^+$ and $pp\to pp\pi^0$ were measured at a beam momentum of 0.95 GeV/c ($T_p \approx$ 400 MeV) using the short version of the COSY-TOF spectrometer. The central calorimeter provided particle identification, energy determination and neutron detection in addition to time-of-flight and angle measurements from other detector parts. Thus all pion production channels were recorded with 1-4 overconstraints. Main emphasis is put on the presentation and discussion of the $np\pi^+$ channel, since the results on the other channels have already been published previously. The total and differential cross sections obtained are compared to theoretical calculations. In contrast to the $pp\pi^0$ channel we find in the $np\pi^+$ channel a strong influence of the $\Delta$ excitation already at this energy close to threshold. In particular we find a $(3 cos^2\Theta + 1)$ dependence in the pion angular distribution, typical for a pure s-channel $\Delta$ excitation and identical to that observed in the $d\pi^+$ channel. Since the latter is understood by a s-channel resonance in the $^1D_2$ $pn$ partial wave, we discuss an analogous scenario for the $pn\pi^+$ channel

Effect of Gemcitabine based chemotherapy on the immunogenicity of pancreatic tumour cells and T-cells.

PURPOSE: Chemotherapy for advanced pancreatic cancer has limited efficacy due to the difficultly of treating established tumours and the evolution of tumour resistance. Chemotherapies for pancreatic cancer are typically studied for their cytotoxic properties rather than for their ability to increase the immunogenicity of pancreatic tumour cells. In this study Gemcitabine in combination with immune modulatory chemotherapies Oxaliplatin, zoledronic acid and pomalidomide was studied to determine how combination therapy alters the immunogenicity of pancreatic tumour cell lines and subsequent T-cell responses. METHODS: Pancreatic tumour cell lines were stimulated with the chemotherapeutic agents and markers of immune recognition were assessed. The effect of chemotherapeutic agents on DC function was measured using uptake of CFSE-stained PANC-1 cells, changes in markers of maturation and their ability to activate CD8+ T-cells. The effect of chemotherapeutic agents on T-cell priming prior to activation using anti-CD3 and anti-CD28 antibodies was determined by measuring IFN-γ expression and Annexin V staining using flow cytometry. RESULTS: These agents demonstrate both additive and inhibitory properties on a range of markers of immunogenicity. Gemcitabine was notable for its ability to induce the upregulation of human leukocyte antigen and checkpoints on pancreatic tumour cell lines whilst inhibiting T-cell activation. Pomalidomide demonstrated immune modulatory properties on dendritic cells and T-cells, even in the presence of gemcitabine. DISCUSSION: These data highlight the complex interactions of different agents in the modulation of tumour immunogenicity and immune cell activation and emphasise the complexity in rationally designing chemo immunogenic combinations for use with immunotherapy