1,772 research outputs found
Calibration Tests of a German Log Rodmeter
A German log rodmeter of the pitot static type was calibrated in Langley tank no. 1 at speeds up to 34 knots and angles of yaw from 0 deg to plus or minus 10 3/4 degrees. The dynamic head approximated the theoretical head at 0 degrees yaw but decreased as the yaw was increased. The static head was negative and in general became more negative with increasing speed and yaw. Cavitation occurred at speeds above 31 knots at 0 deg yaw and 21 knots at 10 3/4 deg yaw
The origin recognition complex in silencing, cell cycle progression, and DNA replication
This report describes the isolation of ORC5, the gene encoding the fifth largest subunit of the origin recognition complex, and the properties of mutants with a defective allele of ORC5. The orc5-1 mutation caused temperature-sensitive growth and, at the restrictive temperature, caused cell cycle arrest. At the permissive temperature, the orc5-1 mutation caused an elevated plasmid loss rate that could be suppressed by additional tandem origins of DNA replication. The sequence of ORC5 revealed a potential ATP binding site, making Orc5p a candidate for a subunit that mediates the ATP-dependent binding of ORC to origins. Genetic interactions among orc2-1 and orc5-1 and other cell cycle genes provided further evidence for a role for the origin recognition complex (ORC) in DNA replication. The silencing defect caused by orc5-1 strengthened previous connections between ORC and silencing, and combined with the phenotypes caused by orc2 mutations, suggested that the complex itself functions in both processes
Excess noise in GaAs and AlGaAs avalanche photodiodes with GaSb absorption regions—composite structures grown using interfacial misfit arrays
Interfacial misfit arrays were embedded within two avalanche photodiode (APD) structures. This allowed GaSb absorption layers to be combined with wide-bandgap multiplication regions, consisting of GaAs and Al0.8Ga0.2As, respectively. The GaAs APD represents the simplest case. The Al0.8Ga0.2As APD shows reduced dark currents of 5.07 μAcm−2 at 90% of the breakdown voltage, and values for effective below 0.2. Random-path-length modeled excess noise is compared with experimental data, for both samples. The designs could be developed further, allowing operation to be extended to longer wavelengths, using other established absorber materials which are lattice matched to GaSb
Plasticity of Foot Muscle and Cardiac Thermal Limits in the Limpet \u3cem\u3eLottia limatula\u3c/em\u3e from Locations with Differing Temperatures
Species distributions are shifting in response to increased habitat temperatures as a result of ongoing climate change. Understanding variation in physiological plasticity among species and populations is important for predicting these distribution shifts. Interspecific variation in intertidal ectotherms’ short-term thermal plasticity has been well established. However, intraspecific variation among populations from differing thermal habitats remains a question pertinent to understanding the effects of climate change on species’ ranges. In this study, we explored upper thermal tolerance limits and plasticity of those limits using a foot muscle metric and 2 cardiac metrics (Arrhenius breakpoint temperature, ABT, and flatline temperature, FLT) in adult file limpets Lottia limatula. Limpets were collected from thermally different coastal and inland-estuarine habitats and held for 2 wk at 13, 17 or 21°C prior to thermal performance assays. Compared to limpets from the warm estuary site, limpets from the cold outer coast site had similar foot muscle critical thermal maxima (CTmax; 35.2 vs. 35.6°C) but lower cardiac thermal tolerances (ABT: 30.5 vs. 35.1°C). Limpets from the cold coast site had higher acclimation responses in foot muscle CTmax (0.22°C per 1°C rise in acclimation) than those of the warm estuary site (0.07°C per 1°C rise in acclimation), but lower acclimation responses in cardiac thermal tolerance (ABT: -0.85°C per 1°C rise in acclimation) than those of the estuary site (ABT: 0.10°C per 1°C rise in acclimation). Since outer coast populations had lower cardiac plasticity and higher mortalities in the warm acclimation, we predict L. limatula from colder habitats will be more susceptible to rising temperatures. Our findings illustrate the importance of population-specific variation in short-term thermal plasticity when considering the effects of climate change on ectotherms
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Kleptoparasitic melees--modelling food stealing featuring contests with multiple individuals
Kleptoparasitism is the stealing of food by one animal from another. This has been modelled in various ways before, but all previous models have only allowed contests between two individuals. We investigate a model of kleptoparasitism where individuals are allowed to fight in groups of more than two, as often occurs in real populations. We find the equilibrium distribution of the population amongst various behavioural states, conditional upon the strategies played and environmental parameters, and then find evolutionarily stable challenging strategies. We find that there is always at least one ESS, but sometimes there are two or more, and discuss the circumstances when particular ESSs occur, and when there are likely to be multiple ESSs
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PP2ARts1 is a master regulator of pathways that control cell size
Cell size checkpoints ensure that passage through G1 and mitosis occurs only when sufficient growth has occurred. The mechanisms by which these checkpoints work are largely unknown. PP2A associated with the Rts1 regulatory subunit (PP2ARts1) is required for cell size control in budding yeast, but the relevant targets are unknown. In this paper, we used quantitative proteome-wide mass spectrometry to identify proteins controlled by PP2ARts1. This revealed that PP2ARts1 controls the two key checkpoint pathways thought to regulate the cell cycle in response to cell growth. To investigate the role of PP2ARts1 in these pathways, we focused on the Ace2 transcription factor, which is thought to delay cell cycle entry by repressing transcription of the G1 cyclin CLN3. Diverse experiments suggest that PP2ARts1 promotes cell cycle entry by inhibiting the repressor functions of Ace2. We hypothesize that control of Ace2 by PP2ARts1 plays a role in mechanisms that link G1 cyclin accumulation to cell growth
An epidemiologic study of early biologic effects of benzene in Chinese workers.
Benzene is a recognized hematotoxin and leukemogen, but its mechanisms of action in humans are still uncertain. To provide insight into these processes, we carried out a cross-sectional study of 44 healthy workers currently exposed to benzene (median 8-hr time-weighted average; 31 ppm), and unexposed controls in Shanghai, China. Here we provide an overview of the study results on peripheral blood cells levels and somatic cell mutation frequency measured by the glycophorin A (GPA) gene loss assay and report on peripheral cytokine levels. All peripheral blood cells levels (i.e., total white blood cells, absolute lymphocyte count, platelets, red blood cells, and hemoglobin) were decreased among exposed workers compared to controls, with the exception of the red blood cell mean corpuscular volume, which was higher among exposed subjects. In contrast, peripheral cytokine levels (interleukin-3, interleukin-6, erythropoietin, granulocyte colony-stimulating factor, tissue necrosis factor-alpha) in a subset of the most highly exposed workers (n = 11) were similar to values in controls (n = 11), suggesting that benzene does not affect these growth factor levels in peripheral blood. The GPA assay measures stem cell or precursor erythroid cell mutations expressed in peripheral red blood cells of MN heterozygous subjects, identifying NN variants, which result from loss of the GPA M allele and duplication of the N allele, and N phi variants, which arise from gene inactivation. The NN (but not N phi) GPA variant cell frequency was elevated in the exposed workers compared with controls (mean +/- SD, 13.9 +/- 8.4 mutants per million cells versus 7.4 +/- 5.2 per million cells, (respectively; p = 0.0002), suggesting that benzene produces gene-duplicating but not gene-inactivating mutations at the GPA locus in bone marrow cells of exposed humans. These findings, combined with ongoing analyses of benzene macromolecular adducts and chromosomal aberrations, will provide an opportunity to comprehensively evaluate a wide range of early biologic effects associated with benzene exposure in humans
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