Structure of d(TGCGCA)(2) and a comparison with other DNA Hexamers

The X-ray crystal structure of d(TGCGCA)(2) has been determined at 120 K to a resolution of 1.3 Angstrom. Hexamer duplexes, in the Z-DNA conformation, pack in an arrangement similar to the 'pure spermine form' [Egli et al. (1991). Biochemistry, 30, 11388-11402] but with significantly different cell dimensions. The phosphate backbone exists in two equally populated discrete conformations at one nucleotide step, around phosphate 11. The structure contains two ordered cobalt hexammine molecules which have roles in stabilization of both the Z-DNA conformation of the duplex and in crystal packing. A comparison of d(TGCGCA)(2) with other Z-DNA hexamer structures available in the Nucleic Acid Database illustrates the elusive nature of crystal packing. A review of the interactions with the metal cations Na+, Mg2+ and Co3+ reveals a relatively small proportion of phosphate binding and that close contacts between metal ions are common. A prediction of the water structure is compared with the observed pattern in the reported structure

Control of Structure in Conventional Friction Stir Welds through a Kinematic Theory of Metal Flow

In friction stir welding (FSW), a rotating pin is translated along a weld seam so as to stir the sides of the seam together. Metal is prevented from flowing up the pin, which would result in plowing/cutting instead of welding, by a shoulder on the pin. In conventional FSW, the weld metal rests on an "anvil", which supports the heavy "plunge" load on the tool. In this study, both embedded tungsten wires along and copper plating on the faying surfaces were used to trace the flow of AA2219 weld metal around the C-FSW tool. The effect of tool rotational speed, travel speed, plunge load, and pin thread pitch on the resulting weld metal flow was evaluated. Plan, longitudinal, and transverse section x-ray radiographs were examined to trace the metal flow paths. The results are interpreted in terms of a kinematic theory of metal flow in FSW

Cosmology with Weak Lensing Surveys

Weak gravitational lensing surveys measure the distortion of the image of distant sources due to the deflections of light rays by the fluctuations of the gravitational potential along the line of sight. Since they probe the non-linear matter power spectrum itself at medium redshift such surveys are complimentary to both galaxy surveys (which follow stellar light) and cosmic microwave background observations (which probe the linear regime at high redshift). Ongoing CMB experiments such as WMAP and the future Planck satellite mission will measure the standard cosmological parameters with unprecedented accuracy. The focus of attention will then shift to understanding the nature of dark matter and vacuum energy: several recent studies suggest that lensing is the best method for constraining the dark energy equation of state. During the next 5 year period ongoing and future weak lensing surveys such as the Joint Dark Energy Mission (JDEM, e.g. SNAP) or the Large-aperture Synoptic Survey Telescope (LSST) will play a major role in advancing our understanding of the universe in this direction. In this review article we describe various aspects of weak lensing surveys and how they can help us in understanding our universe.Comment: 15 pages, review article to appear in 2005 Triennial Issue of Phil. Trans.

Economics of dialysis dependence following renal replacement therapy for critically ill acute kidney injury patients.

BACKGROUND: The obective of this study was to perform a cost-effectiveness analysis comparing intermittent with continuous renal replacement therapy (IRRT versus CRRT) as initial therapy for acute kidney injury (AKI) in the intensive care unit (ICU). METHODS: Assuming some patients would potentially be eligible for either modality, we modeled life year gained, the quality-adjusted life years (QALYs) and healthcare costs for a cohort of 1000 IRRT patients and a cohort of 1000 CRRT patients. We used a 1-year, 5-year and a lifetime horizon. A Markov model with two health states for AKI survivors was designed: dialysis dependence and dialysis independence. We applied Weibull regression from published estimates to fit survival curves for CRRT and IRRT patients and to fit the proportion of dialysis dependence among CRRT and IRRT survivors. We then applied a risk ratio reported in a large retrospective cohort study to the fitted CRRT estimates in order to determine the proportion of dialysis dependence for IRRT survivors. We conducted sensitivity analyses based on a range of differences for daily implementation cost between CRRT and IRRT (base case: CRRT day $632 more expensive than IRRT day; range from$200 to $1000) and a range of risk ratios for dialysis dependence for CRRT as compared with IRRT (from 0.65 to 0.95; base case: 0.80). RESULTS: Continuous renal replacement therapy was associated with a marginally greater gain in QALY as compared with IRRT (1.093 versus 1.078). Despite higher upfront costs for CRRT in the ICU ($4046 for CRRT versus $1423 for IRRT in average), the 5-year total cost including the cost of dialysis dependence was lower for CRRT ($37 780 for CRRT versus $39 448 for IRRT on average). The base case incremental cost-effectiveness analysis showed that CRRT dominated IRRT. This dominance was confirmed by extensive sensitivity analysis. CONCLUSIONS: Initial CRRT is cost-effective compared with initial IRRT by reducing the rate of long-term dialysis dependence among critically ill AKI survivors

Positive pion absorption on 3He using modern trinucleon wave functions

We study pion absorption on 3He employing trinucleon wave functions calculated from modern realistic NN interactions (Paris, CD Bonn). Even though the use of the new wave functions leads to a significant improvement over older calculations with regard to both cross section and polarization data, there are hints that polarization data with quasifree kinematics cannot be described by just two-nucleon absorption mechanisms.Comment: 14 pages, 6 figure

The Importance of Time Congruity in the Organisation.

In 1991 Kaufman, Lane, and Lindquist proposed that time congruity in terms of an individual's time preferences and the time use methods of an organisation would lead to satisfactory performance and enhancement of quality of work and general life. The research reported here presents a study which uses commensurate person and job measures of time personality in an organisational setting to assess the effects of time congruity on one aspect of work life, job-related affective well-being. Results show that time personality and time congruity were found to have direct effects on well-being and the influence of time congruity was found to be mediated through time personality, thus contributing to the person–job (P–J) fit literature which suggests that direct effects are often more important than indirect effects. The study also provides some practical examples of ways to address some of the previously cited methodological issues in P–J fit research

Practical limitations to indentation testing of thin films

A method that is becoming increasingly common for measuring the mechanical behavior of thin films is low-load indentation testing. However, there can be complications in interpreting the results as many factors can affect hardness and moduli measurements such as surface roughness and determination of the indentation contact area. To further the understanding, the mechanical properties of thin (50 nm) films of AlN on sapphire substrates were evaluated using a scanning force microscopy (SFM) based pico-indentation device to allow imaging of the surface and indentations. The primary emphasis was the types of problems or limitations involved in testing very thin, as deposited films in which properties are desired over indentation depths less than 50 nm

Critical-point scaling function for the specific heat of a Ginzburg-Landau superconductor

If the zero-field transition in high temperature superconductors such as YBa_2Cu_3O_7-\delta is a critical point in the universality class of the 3-dimensional XY model, then the general theory of critical phenomena predicts the existence of a critical region in which thermodynamic functions have a characteristic scaling form. We report the first attempt to calculate the universal scaling function associated with the specific heat, for which experimental data have become available in recent years. Scaling behaviour is extracted from a renormalization-group analysis, and the 1/N expansion is adopted as a means of approximation. The estimated scaling function is qualitatively similar to that observed experimentally, and also to the lowest-Landau-level scaling function used by some authors to provide an alternative interpretation of the same data. Unfortunately, the 1/N expansion is not sufficiently reliable at small values of N for a quantitative fit to be feasible.Comment: 20 pages; 4 figure