Anomalous x-ray scattering from terbium-labeled parvalbumin in solution

We have used anomalous small-angle x-ray scattering as a structural probe for solutions of rabbit parvalbumin labeled with terbium. This technique makes use of the large changes in the terbium scattering factor that occur when the x-ray energy is tuned around an L3 absorption edge of this heavy-atom label. These changes in scattering result in changes in the small-angle scattering curve of the labeled protein as a whole, which can then be analyzed to derive structural information concerning the distribution of labels in the protein. Based on a Gaussian model for the protein electron density, the mean distance from the terbiums to the protein center of mass is determined to be 13.2 A and is consistent with crystallographic results. Our results demonstrate the usefulness of terbium as an anomalous scattering label and provide criteria to help establish anomalous scattering as a reliable structural technique for proteins in solution

Repetitive Segmental Structure of the Transducin β Subunit: Homology with the CDC4 Gene and Identification of Related mRNAs

Retinal transducin, a guanine nucleotide regulatory protein (referred to as a G protein) that activates a cGMP phosphodiesterase in photoreceptor cells, is comprised of three subunits. We have identified and analyzed cDNA clones of the bovine transducin β subunit that may be highly conserved or identical to that in other G proteins. From the cDNA nucleotide sequence of the entire coding region, the primary structure of a 340-amino acid protein was deduced. The encoded β subunit has a Mr of 37,375 and is comprised of repetitive homologous segments arranged in tandem. Furthermore, significant homology in primary structure and segmental sequence exists between the β subunit and the yeast CDC4 gene product. The Mr 37,375 β subunit polypeptide is encoded by a 2.9-kilobase (kb) mRNA. However, there exists in retina other β-related mRNAs that are divergent from the 2.9-kb mRNA on the basis of oligonucleotide and primer-extended probe hybridizations. All mammalian tissues and clonal cell lines that have been examined contain at least two β-related mRNAs, usually 1.8 and 2.9 kb in length. These results suggest that the mRNAs are the processed products of a small number of closely related genes or of a single highly complex β gene

Bacillus-Shaped Deposits Composed of Hexahedrally Based Crystals in Human Dental Calculus

In human supra- and subgingival calculus, bacillus-shaped deposits showing various rocky-pile forms composed of hexahedrally based crystals were observed by scanning electron microscopy. The crystal size measured approximately 0.1 - 1.5 μm. The electron probe microanalysis always detected calcium, phosphorous, and magnesium. Their molar ratios resembled those of magnesium-containing whitlockite and moreover the crystals also gave the electron diffraction pattern of whitlockite. The bacillus-shaped deposits happened to coexist with the intracellular calcifying microorganisms, furthermore, oral microorganisms partially replaced by the hexahedrally based crystals were found. The crystal deposits were never seen in the surface layers of calculus exposed to the oral cavity, but occurred in the innermost layers and intra-spaces of supragingival and ledge-type subgingival calculus and in the outer layers of deep subgingival calculus

Λ c + production in pp collisions at√ s=7 TeV and in p-Pb collisions at√ sNN=5.02 TeV

The pT-differential production cross section of prompt Λ c + charmed baryons was measured with the ALICE detector at the Large Hadron Collider (LHC) in pp collisions at s√=7 TeV and in p-Pb collisions at sNN−−−√=5.02 TeV at midrapidity. The Λ c + and Λ¯¯¯¯c¯¯¯ were reconstructed in the hadronic decay modes Λ c +  → pK−π+, Λ c +  → pK S 0 and in the semileptonic channel Λ c +  → e+νeΛ (and charge conjugates). The measured values of the Λ c + /D0 ratio, which is sensitive to the c-quark hadronisation mechanism, and in particular to the production of baryons, are presented and are larger than those measured previously in different colliding systems, centre-of-mass energies, rapidity and pT intervals, where the Λ c + production process may differ. The results are compared with the expectations obtained from perturbative Quantum Chromodynamics calculations and Monte Carlo event generators. Neither perturbative QCD calculations nor Monte Carlo models reproduce the data, indicating that the fragmentation of heavy-flavour baryons is not well understood. The first measurement at the LHC of the Λ c + nuclear modification factor, RpPb, is also presented. The RpPb is found to be consistent with unity and with that of D mesons within the uncertainties, and consistent with a theoretical calculation that includes cold nuclear matter effects and a calculation that includes charm quark interactions with a deconfined medium

Stratospheric aircraft exhaust plume and wake chemistry studies

This report documents progress to date in an ongoing study to analyze and model emissions leaving a proposed High Speed Civil Transport (HSCT) from when the exhaust gases leave the engine until they are deposited at atmospheric scales in the stratosphere. Estimates are given for the emissions, summarizing relevant earlier work (CIAP) and reviewing current propulsion research efforts. The chemical evolution and the mixing and vortical motion of the exhaust are analyzed to track the exhaust and its speciation as the emissions are mixed to atmospheric scales. The species tracked include those that could be heterogeneously reactive on the surfaces of the condensed solid water (ice) particles and on exhaust soot particle surfaces. Dispersion and reaction of chemical constituents in the far wake are studied with a Lagrangian air parcel model, in conjunction with a radiation code to calculate the net heating/cooling. Laboratory measurements of heterogeneous chemistry of aqueous sulfuric acid and nitric acid hydrates are also described. Results include the solubility of HCl in sulfuric acid which is a key parameter for modeling stratospheric processing. We also report initial results for condensation of nitric acid trihydrate from gas phase H2O and HNO3

Measurements of e(+)e(-) pairs from open heavy flavor in p plus p and d plus A collisions at root S-NN=200 GeV

We report a measurement of e+e− pairs from semileptonic heavy-flavor decays in p+p collisions at √sNN=200 GeV. The e+e− pair yield from b¯b and c¯c is separated by exploiting a double differential fit done simultaneously in dielectron invariant mass and pT. We used three different event generators, pythia, mc@nlo, and powheg, to simulate the e+e− spectra from c¯c and b¯b production. The data can be well described by all three generators within the detector acceptance. However, when using the generators to extrapolate to 4π, significant differences are observed for the total cross section. These difference are less pronounced for b¯b than for c¯c. The same model dependence was observed in already published d+A data. The p+p data are also directly compared with d+A data in mass and pT, and within the statistical accuracy no nuclear modification is seen

Lévy-stable two-pion Bose-Einstein correlations in √sNN=200 GeV Au+Au collisions

We present a detailed measurement of charged two-pion correlation functions in 0–30% centrality √sNN=200 GeV Au+Au collisions by the PHENIX experiment at the Relativistic Heavy Ion Collider. The data are well described by Bose-Einstein correlation functions stemming from Lévy-stable source distributions. Using a fine transverse momentum binning, we extract the correlation strength parameter λ, the Lévy index of stability α, and the Lévy length scale parameter R as a function of average transverse mass of the pair mT. We find that the positively and the negatively charged pion pairs yield consistent results, and their correlation functions are represented, within uncertainties, by the same Lévy-stable source functions. The λ(mT) measurements indicate a decrease of the strength of the correlations at low mT. The Lévy length scale parameter R(mT) decreases with increasing mT, following a hydrodynamically predicted type of scaling behavior. The values of the Lévy index of stability α are found to be significantly lower than the Gaussian case of α=2, but also significantly larger than the conjectured value that may characterize the critical point of a second-order quark-hadron phase transition

Measurements of double-helicity asymmetries in inclusive J／ψ production in longitudinally polarized p+p collisions at √s=510 GeV

We report the double-helicity asymmetry, AJ/ψLL, in inclusive J/ψ production at forward rapidity as a function of transverse momentum pT and rapidity |y|. The data analyzed were taken during √s=510  GeV longitudinally polarized p+p collisions at the Relativistic Heavy Ion Collider in the 2013 run using the PHENIX detector. At this collision energy, J/ψ particles are predominantly produced through gluon-gluon scatterings, thus AJ/ψLL is sensitive to the gluon polarization inside the proton. We measured AJ/ψLL by detecting the decay daughter muon pairs μ+μ− within the PHENIX muon spectrometers in the rapidity range 1.2<|y|<2.2. In this kinematic range, we measured the AJ/ψLL to be 0.012±0.010 (stat) ±0.003 (syst). The AJ/ψLL can be expressed to be proportional to the product of the gluon polarization distributions at two distinct ranges of Bjorken x: one at moderate range x≈5×10−2 where recent data of jet and π0 double helicity spin asymmetries have shown evidence for significant gluon polarization, and the other one covering the poorly known small-x region x≈2×10−3. Thus our new results could be used to further constrain the gluon polarization for x<5×10−2

J/ψ Elliptic Flow in Pb-Pb Collisions at √sNN=5.02 TeV

We report a precise measurement of the J/ψ elliptic flow in Pb-Pb collisions at √sNN=5.02  TeV with the ALICE detector at the LHC. The J/ψ mesons are reconstructed at midrapidity (|y|<0.9) in the dielectron decay channel and at forward rapidity (2.5<y<4.0) in the dimuon channel, both down to zero transverse momentum. At forward rapidity, the elliptic flow v2 of the J/ψ is studied as a function of the transverse momentum and centrality. A positive v2 is observed in the transverse momentum range 2<pT<8  GeV/c in the three centrality classes studied and confirms with higher statistics our earlier results at √sNN=2.76  TeV in semicentral collisions. At midrapidity, the J/ψ  v2 is investigated as a function of the transverse momentum in semicentral collisions and found to be in agreement with the measurements at forward rapidity. These results are compared to transport model calculations. The comparison supports the idea that at low pT the elliptic flow of the J/ψ originates from the thermalization of charm quarks in the deconfined medium but suggests that additional mechanisms might be missing in the models

J/ψ production as a function of charged-particle pseudorapidity density in p–Pb collisions at √sNN=5.02 TeV

We report measurements of the inclusive J/ψ yield and average transverse momentum as a function of charged-particle pseudorapidity density dNch/dη in p–Pb collisions at √sNN=5.02 TeV with ALICE at the LHC. The observables are normalised to their corresponding averages in non-single diffractive events. An increase of the normalised J/ψ yield with normalised dNch/dη, measured at mid-rapidity, is observed at mid-rapidity and backward rapidity. At forward rapidity, a saturation of the relative yield is observed for high charged-particle multiplicities. The normalised average transverse momentum at forward and backward rapidities increases with multiplicity at low multiplicities and saturates beyond moderate multiplicities. In addition, the forward-to-backward nuclear modification factor ratio is also reported, showing an increasing suppression of J/ψ production at forward rapidity with respect to backward rapidity for increasing charged-particle multiplicity